Platelet concentrate transport in pneumatic tube systems – does it work?

Lancé, Marcus D.; Marcus, Marco A. E.; Oerle, René van; Theunissen, H. Maurice S.; Henskens, Yvonne

doi:10.1111/j.1423-0410.2011.01580.x

Cited by 18 publications

(18 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon PLT activation, P-selectin, a protein in the agranule of PLTs, becomes exposed on the PLT surface [28]. In agreement with previous studies [13,15,[31][32][33], PLTs in the present study became spontaneously activated during storage as measured as the percentage of P-selectin-positive. Spontaneous activation of GPIIb/IIIa was less pronounced and did to some degree parallel the spontaneous aggregation, suggesting that the main mechanism behind unstimulated aggregation mechanism might be GBIIb/IIIa activation.…”

Section: Discussionsupporting

confidence: 92%

“…Nonetheless, the PLT aggregation capacity of WB is at least as good as it is in BCP or AP. Previous studies have shown reduced PLT aggregation capacity in BCP and AP during storage as measured by light transmission aggregometry [12][13][14][15][16][17][18] or multiple electrode aggregometry [12,13,19,20], but only few have compared PLT function in BCP or AP to that of WB [23,24]. Knowledge of PLT function in WB during storage is sparse and has shown conflicting results [21][22][23].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown that PLT function tends to decrease during storage at room temperature [12][13][14][15][16][17][18][19][20]. A few studies have examined PLT activity in WB and found contradicting results depending on the agonist used [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effects of storage on platelet function in different blood products

et al. 2018

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The effects of storage on platelet function in different blood products

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Rapid acceleration and deceleration may induce excessive vibration, denature proteins and result in haemolysis, platelet activation, and other effects [4, 35, 63]. Clinical decisions regarding platelet function and aspirin responsiveness should not be based on blood specimens transported by a PTS [65–68] (Table 3). …”

Section: Methodsmentioning

confidence: 99%

Pre-analytical issues in the haemostasis laboratory: guidance for the clinical laboratories

Magnette¹,

Chatelain²,

Châtelain³

et al. 2016

Thrombosis J

103

View full text Add to dashboard Cite

Ensuring quality has become a daily requirement in laboratories. In haemostasis, even more than in other disciplines of biology, quality is determined by a pre-analytical step that encompasses all procedures, starting with the formulation of the medical question, and includes patient preparation, sample collection, handling, transportation, processing, and storage until time of analysis. This step, based on a variety of manual activities, is the most vulnerable part of the total testing process and is a major component of the reliability and validity of results in haemostasis and constitutes the most important source of erroneous or un-interpretable results.Pre-analytical errors may occur throughout the testing process and arise from unsuitable, inappropriate or wrongly handled procedures. Problems may arise during the collection of blood specimens such as misidentification of the sample, use of inadequate devices or needles, incorrect order of draw, prolonged tourniquet placing, unsuccessful attempts to locate the vein, incorrect use of additive tubes, collection of unsuitable samples for quality or quantity, inappropriate mixing of a sample, etc. Some factors can alter the result of a sample constituent after collection during transportation, preparation and storage.Laboratory errors can often have serious adverse consequences. Lack of standardized procedures for sample collection accounts for most of the errors encountered within the total testing process. They can also have clinical consequences as well as a significant impact on patient care, especially those related to specialized tests as these are often considered as “diagnostic”. Controlling pre-analytical variables is critical since this has a direct influence on the quality of results and on their clinical reliability. The accurate standardization of the pre-analytical phase is of pivotal importance for achieving reliable results of coagulation tests and should reduce the side effects of the influence factors. This review is a summary of the most important recommendations regarding the importance of pre-analytical factors for coagulation testing and should be a tool to increase awareness about the importance of pre-analytical factors for coagulation testing.

show abstract

“…A useful method for testing PCs in a clinical setting would need to be sensitive for variation in platelet function, correlate with post-transfusion platelet function, be reproducible irrespective of operator experience, and be time and labor efficient [ 10 ]. Although we recently showed that LTA is capable of testing platelet function in PCs, it does not fit the desired characteristics of a useful test in a clinical setting [ 11 , 12 ]. Additionally, there is little data to show that LTA can predict in vivo recovery of transfused platelets [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Application of an optimized flow cytometry-based quantification of Platelet Activation (PACT): Monitoring platelet activation in platelet concentrates

et al. 2017

View full text Add to dashboard Cite

BackgroundPrevious studies have shown that flow cytometry is a reliable test to quantify platelet function in stored platelet concentrates (PC). It is thought that flow cytometry is laborious and hence expensive. We have optimized the flow cytometry-based quantification of agonist induced platelet activation (PACT) to a labor, time and more cost-efficient test. Currently the quality of PCs is only monitored by visual inspection, because available assays are unreliable or too laborious for use in a clinical transfusion laboratory. Therefore, the PACT was applied to monitor PC activation during storage.Study design and methodsThe optimized PACT was used to monitor 5 PCs during 10 days of storage. In brief, optimized PACT uses a ready-to-use reaction mix, which is stable at -20°C. When needed, a test strip is thawed and platelet activation is initiated by mixing PC with PACT. PACT was based on the following agonists: adenosine diphosphate (ADP), collagen-related peptide (CRP) and thrombin receptor-activating peptide (TRAP-6). Platelet activation was measured as P-selectin expression. Light transmission aggregometry (LTA) was performed as a reference.ResultsBoth PACT and LTA showed platelet function decline during 10-day storage after stimulation with ADP and collagen/CRP; furthermore, PACT showed decreasing TRAP-induced activation. Major differences between the two tests are that PACT is able to measure the status of platelets in the absence of agonists, and it can differentiate between the number of activated platelets and the amount of activation, whereas LTA only measures aggregation in response to an agonist. Also, PACT is more time-efficient compared to LTA and allows high-throughput analysis.ConclusionPACT is an optimized platelet function test that can be used to monitor the activation of PCs. PACT has the same accuracy as LTA with regard to monitoring PCs, but it is superior to both LTA and conventional flow cytometry based tests with regard to labor-, time- and cost efficiency.

show abstract

Platelet concentrate transport in pneumatic tube systems – does it work?

Cited by 18 publications

References 10 publications

The effects of storage on platelet function in different blood products

The effects of storage on platelet function in different blood products

Pre-analytical issues in the haemostasis laboratory: guidance for the clinical laboratories

Application of an optimized flow cytometry-based quantification of Platelet Activation (PACT): Monitoring platelet activation in platelet concentrates

Contact Info

Product

Resources

About