Platelet-derived growth factor-BB supports the survival of cultured rat schwann cell precursors in synergy with neurotrophin-3

Lobsiger, Christian S.; Schweitzer, Beat; Taylor, Verdon; Suter, Ueli

doi:10.1002/(sici)1098-1136(200005)30:3<290::aid-glia8>3.0.co;2-6

Cited by 42 publications

(33 citation statements)

References 43 publications

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“…1 E, red diamonds and green squares, respectively), consistent with previous studies (Kuhlbrodt et al, 1998;Lobsiger et al, 2000;Paratore et al, 2001). The O4 antigen sulfatide is a differentiation marker absent from SC precursors but present in ϳ90% of early SCs isolated at E16 (Dong et al, 1999).…”

Section: Characterization Of Gfp-positive Cellssupporting

confidence: 91%

“…SC precursors die within 24 hr in culture if not provided with specific growth factors, whereas early SCs survive (Jessen et al, 1994;Dong et al, 1999;Lobsiger et al, 2000). As expected, survival capacity of sorted cells was acquired between E12 and E16, with the E14 stage being intermediate ( Fig.…”

Section: Characterization Of Gfp-positive Cellssupporting

confidence: 66%

“…Culture of NCSCs at medium density (1000 -2000/5 l) was performed as reported previously (Stemple and Anderson, 1992;Hagedorn et al, 1999). Embryonic SCs were cultured as reported previously (Jessen et al, 1994;Dong et al, 1999;Lobsiger et al, 2000) at a density of 250 -500 cells/5 l. In both cases, ϳ25% of sorted cells attached. Short-term cultures were fixed after 3-6 hr.…”

Section: Methodsmentioning

confidence: 99%

“…Short-term cultures were fixed after 3-6 hr. Survival assays were performed as described previously (Jessen et al, 1994;Dong et al, 1999;Lobsiger et al, 2000). Results are expressed as percentage of p75 ϩ cells surviving after 24 hr.…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemistry on dissociated cells was performed as described (Lobsiger et al, 2000;Paratore et al, 2001). Antibodies were against O4 (mouse monoclonal, IgM, 1:10 dilution; from M. Schwab, Brain Research Institute, Zürich, Switzerland), p75ex (rabbit anti-mouse p75 extracellular domain, 1:500; Calbiochem, Lucerne, Switzerland), and Sox10 (mouse monoclonal, 1:3; from M. Wegner, University of Erlangen, Erlangen, Germany).…”

Section: Methodsmentioning

confidence: 99%

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Efficient Isolation and Gene Expression Profiling of Small Numbers of Neural Crest Stem Cells and Developing Schwann Cells

Buchstaller

Sommer²,

Bodmer³

et al. 2004

J. Neurosci.

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Schwann cells develop from multipotent neural crest stem cells and are important for neuronal survival, maintenance of axonal integrity, and myelination. We used transgenic mice expressing green fluorescent protein in a tissue-specific manner to isolate viable, pure populations of neural crest stem cells and developing Schwann cells, which are not readily accessible by microdissection. Starting with the minute amounts of RNA obtained, a two-round amplification procedure was used to achieve reproducible DNA array hybridizations. We validated our screening procedure by comparisons with the literature and by in situ hybridization. Stage-to-stage comparisons and hierarchical clustering for neural crest and five stages of Schwann cell development suggest a wealth of candidates for genes involved in stem cell regulation and in early Schwann cell development. The combination of methods applied in this study should be generally useful for isolating and profiling other stem cell and difficult to isolate cell populations.

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Section: Characterization Of Gfp-positive Cellssupporting

confidence: 91%

Section: Characterization Of Gfp-positive Cellssupporting

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Efficient Isolation and Gene Expression Profiling of Small Numbers of Neural Crest Stem Cells and Developing Schwann Cells

Buchstaller

Sommer²,

Bodmer³

et al. 2004

J. Neurosci.

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Localization of TrkC to Schwann cells and effects of neurotrophin‐3 signaling at neuromuscular synapses

Hess

Scott

Potluri

et al. 2007

J of Comparative Neurology

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Neurotrophins and their receptors, the Trks, are differentially expressed among the cell types that make up neuromuscular and other synapses, but the function and directionality of neurotrophin signaling at synapses are poorly understood. Here we demonstrate, via immunostaining, Western blotting, and RT-PCR analyses, that TrkC, the receptor for neurotrophin-3 (NT3), is expressed by mouse perisynaptic and myelinating Schwann cells from birth through adulthood and is unaltered after denervation. Analyses of transgenic mice in which the NT3 coding sequence is replaced by lacZ showed that NT3 is expressed in motor neurons and Schwann cells during perinatal development, but not in adult mice. In muscle, NT3 is expressed by intrafusal muscle fibers within spindles, as has been previously reported. Surprisingly, NT3 is also expressed in extrafusal muscle fibers during perinatal life and in adults. Genetic approaches were used to explore the roles of NT3 and TrkC signaling at neuromuscular synapses. Overexpression of NT3 in muscle fibers during development resulted in an increased number of perisynaptic Schwann cells at neuromuscular synapses, without altering synaptic size, suggesting that muscle-derived NT3 might act as a mitogen or trophic factor for Schwann cells. Conditional deletion of NT3 from motor neurons did not alter the number of Schwann cells or other aspects of neuromuscular synaptic structure, suggesting that motor-neuron-derived NT3 is not required for normal development of perisynaptic Schwann cells or synapses. Together, these results demonstrate that NT3 expression is developmentally regulated in skeletal muscle and may modulate the number of Schwann cells at neuromuscular synapses.

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Platelet‐derived growth factor‐b expression induced after rat peripheral nerve injuries

Oya

Zhao

Takagawa

et al. 2002

Glia

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Schwann cells are crucially important for peripheral nerve regeneration. These cells synthesize several factors that are supposed to enhance axonal regeneration when injured. Platelet-derived growth factor (PDGF) B-chain and its beta-receptor are expressed in Schwann cells in both normal peripheral nerves and culture. To elucidate the role of PDGF-B in peripheral nerve regeneration, we investigated its expression in cut or crush-injured rat sciatic nerves for up to 28 days. Northern blotting identified substantial increase of PDGF B-chain transcripts in injured nerves. Immunohistochemistry demonstrated that protein products of the transcripts were augmented at the distal tip of swollen axons in proximal nerve segments and in regenerating axons. Soon after both types of injury, considerable amounts of PDGF-B accumulated in numerous Schwann cells in distal segments of both models. With restoration of the axon-Schwann cell relationship in the crush model, levels of PDGF-B tended to decrease, eventually returning to normal. In the cut model in which the relationship cannot be restored, the PDGF-B was depleted to a very low level. The spatiotemporal correlation between PDGF-B and cell proliferation was very close throughout the study. These results differed strikingly from those of our previous study of rat optic nerve transection, in which PDGF-B was expressed only in a few recruited macrophages and glial cells. Augmented PDGF-B expression after sciatic nerve injury might contribute to peripheral nerve regeneration because PDGF-B is a mitogen and survival factor for Schwann cells and because it has trophic activity on neurons.

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Platelet-derived growth factor-BB supports the survival of cultured rat schwann cell precursors in synergy with neurotrophin-3

Cited by 42 publications

References 43 publications

Efficient Isolation and Gene Expression Profiling of Small Numbers of Neural Crest Stem Cells and Developing Schwann Cells

Efficient Isolation and Gene Expression Profiling of Small Numbers of Neural Crest Stem Cells and Developing Schwann Cells

Localization of TrkC to Schwann cells and effects of neurotrophin‐3 signaling at neuromuscular synapses

Platelet‐derived growth factor‐b expression induced after rat peripheral nerve injuries

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