Platelet-Derived Growth Factors and Growth-Related Genes in Rat Lung. III. Immunolocalization during Fetal Development

Han, Rui; Mawdsley, C; Souza, P.; Tanswell, A. Keith; Post, Martin

doi:10.1203/00006450-199204000-00004

Cited by 60 publications

(23 citation statements)

References 7 publications

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“…PDGF-AA is required for branching, and PDGF-BB for the parallel increase in lung mass seen during the branching process (16). In other studies, we have demonstrated positive PDGF-BB immunoreactivity in the epithelial cells of the developing airways and peripheral lung at the late canalicular stage of fetal rat lung development (d 20 of 22), which then declines before birth (17). We have also documented the responsiveness of fetal and neonatal lung epithelial cells to exogenous PDGF-BB in vitro (18 -20).…”

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confidence: 88%

Changes in Expression of Platelet-Derived Growth Factor and Its Receptors in the Lungs of Newborn Rats Exposed to Air or 60% O2

et al. 2000

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confidence: 88%

Changes in Expression of Platelet-Derived Growth Factor and Its Receptors in the Lungs of Newborn Rats Exposed to Air or 60% O2

et al. 2000

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“…Although a few reports describing the immunolocalization of PDGFRa have been published (Bidwell et al 1995;Han et al 1992), none used monospecific MAbs. Table 1 compares our results with those of in situ hybridization.…”

Section: Discussionmentioning

confidence: 99%

“…Results of wholemount in situ hybridization of the murine embryo have not been presented, although several studies have used this procedure in tissue sections. No monoclonal antibodies (MAbs) against murine PDGFRs have been produced, although several polyclonal Abs have been raised (Bidwell et al 1995;Han et al 1992).…”

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confidence: 99%

PDGFRα Expression During Mouse Embryogenesis: Immunolocalization Analyzed by Whole-mount Immunohistostaining Using the Monoclonal Anti-mouse PDGFRα Antibody APA5

Takakura

Yoshida

Ogura

et al. 1997

J Histochem Cytochem.

141

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SUMMARYWe investigated the cells that express platelet-derived growth factor receptor ␣ (PDGFR ␣) during mouse embryogenesis. PDGFR ␣ expression has been identified by in situ hybridization or immunohistochemistry using polyclonal antibodies on tissue sections. Because no immunostaining study using whole-mount specimens has been published to date, we established a new monoclonal antibody (MAb), APA5, for this purpose. Our results differed in that APA5 stained only the paraxial mesoderm, whereas other investigators concluded that most if not all mesodermal cells expressed PDGFR ␣. Moreover, we did not find PDGFR ␣ expression in embryonic erythrocytes, which have been previously suggested to express PDGFR ␣. On the basis of our present results, we wish to revise the proposed PDGFR ␣ expression as follows. At the pregastrulation stage, PDGFR ␣ is expressed only in primitive endoderm, particularly that in the ectoplacental cone. On gastrulation, it is expressed at high levels in the paraxial mesoderm. This expression continues after its differentiation into the somite. Along with the differentiation and migration of the sclerotome, PDGFR ␣ ϩ cells begin to become distributed throughout the embryonal mesenchyme. During organogenesis, particularly intense staining is detected in regions of epithelial and mesenchymal interaction, such as the tooth bud and bronchi. In addition to mesodermal derivatives, the developing lens, apical ectodermal ridge, glial precursor, cardiac valves, and choroid plexus express PDGFR ␣. Our results with whole-mount immunostaining show that PDGFR ␣ is abundantly expressed and may play important roles during embryogenesis.

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“…PDGF is a powerful stimulator of fibroblast proliferation and collagen production (23). Han et al (24) reported that both PDGF and its receptors are present in the fetal rat lung. PDGF exists as a homodimer or heterodimer consisting of two polypeptide chains, denoted A and B, which can be dimerized via sulfhydryl bridges to form three bioactive isoforms (12).…”

Section: Discussionmentioning

confidence: 99%

Retinoic Acid Fails to Reverse Oligohydramnios-Induced Pulmonary Hypoplasia in Fetal Rats

et al. 2007

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All-trans retinoic acid (ATRA) stimulates plateletderived growth factor (PDGF)-A expression and enhances alveolarization in rat lungs. On d 16 of gestation, pregnant Sprague-Dawley rats were randomly assigned to either a retinoic acid group (intragastric ATRA at 10 mg/kg body weight) or a vehicle group. We punctured each amniotic sac, and fetuses in the opposite uterine horn served as controls. On d 21 of gestation, the fetuses were delivered by cesarean section. Rats subjected to oligohydramnios exhibited significantly lower lung weights and lung/body weight ratios, and ATRA had no effects on the body or lung weights of oligohydramnios-exposed rats. Lung PDGF-A and -B mRNA expression was significantly lower in oligohydramnios-exposed rats compared with control littermates of maternal vehicle-treated dams. Maternal retinoic acid treatment significantly increased PDGF-A and -B mRNA expression in control and oligohydramnios-exposed rats compared with all rats and oligohydramnios-exposed rats of maternal vehicletreated dams, respectively. Rats exposed to oligohydramnios exhibited a significantly lower generation of alveolar saccules than did control rats in the maternal retinoic acid-and vehicle-treated groups. In this model, maternal retinoic acid treatment showed no positive effects on oligohydramnios-induced pulmonary hypoplasia in the pseudoglandular stage. (Pediatr Res 62: 553-558, 2007) P ulmonary hypoplasia is common in the perinatal period and a significant cause of death in newborn infants (1,2). Oligohydramnios is one of the most commonly associated abnormalities. Oligohydramnios may retard fetal lung growth and can result in pulmonary hypoplasia in experimental animals (3-5) and in human fetuses with prolonged rupture of the membranes (6). Currently, no specific therapies are clinically available to correct the lung hypoplasia in fetuses with oligohydramnios.The mechanism that arrests lung development in the setting of oligohydramnios is not clear. Physical forces play important roles in regulating fetal lung growth and maturation (7,8). The main physical force the lung experience is stretching produced by fetal breathing movements and lung fluids in the airspaces during normal lung development (9). Mechanical stretching may increase growth factor expression through intracellular signal transduction pathways (10). Plateletderived growth factor (PDGF) is important for alveogenesis and angiogenesis of the normally developing lung (11). PDGFs are homodimers or heterodimers consisting of two distinct polypeptide chains (A and B), which can be dimerized via sulfhydryl bridges to form three bioactive isoforms (AA, BB, and AB) (12). Recently, we found pulmonary hypoplasia with reduced radial saccular counts and decreased PDGF-A and -B expression in oligohydramnios-exposed fetal rats (13). Liebeskind et al. (14) noted that all-trans retinoic acid (ATRA) might stimulate PDGF-A mRNA expression in cultured fetal and postnatal rat lung fibroblasts and in newborn rat lungs. Administration of ATRA, a metabo...

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Platelet-Derived Growth Factors and Growth-Related Genes in Rat Lung. III. Immunolocalization during Fetal Development

Cited by 60 publications

References 7 publications

Changes in Expression of Platelet-Derived Growth Factor and Its Receptors in the Lungs of Newborn Rats Exposed to Air or 60% O2

Changes in Expression of Platelet-Derived Growth Factor and Its Receptors in the Lungs of Newborn Rats Exposed to Air or 60% O2

PDGFRα Expression During Mouse Embryogenesis: Immunolocalization Analyzed by Whole-mount Immunohistostaining Using the Monoclonal Anti-mouse PDGFRα Antibody APA5

Retinoic Acid Fails to Reverse Oligohydramnios-Induced Pulmonary Hypoplasia in Fetal Rats

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