Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients

Role of Platelet-Derived Microvesicles As Crosstalk Mediators in Atherothrombosis and Future Pharmacology Targets: A Link between Inflammation, Atherosclerosis, and Thrombosis

“…In vitro cell culture studies provide evidence that pMV promote cell proliferation and survival, migration, and tube formation in human umbilical vein EC via GPCR and kinase signaling pathways (Kim et al, 2004). Similarly, pMV augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients through a RANTES-mediated mechanism (Ohtsuka et al, 2013). pMVs induce sprouting both in vivo and in vitro (Brill et al, 2005) and influence the angiogenic activity of EPC (Prokopi et al, 2009).…”

Circulating angiogenic cell function is inhibited by cortisol in vitro and associated with psychological stress and cortisol in vivo

“…CAC migration in vitro reflects the capacity of CACs to migrate toward sites of tissue damage and promote repair via paracrine secretion of growth factors. CAC migration is decreased in patients with coronary artery disease (Vasa et al, 2001), atherosclerosis (Ohtsuka et al, 2013), diabetes (Thum et al, 2007), and older age (Chen et al, 2016). Among healthy individuals without cardiovascular disease or diabetes, reduced CAC migration prospectively predicts greater carotid artery intima-media thickness (Keymel et al, 2008) and correlates with metabolic risk factors (Aschbacher et al, 2012a) and better endothelial function (Van Craenenbroeck et al, 2010).…”

WITHDRAWN: "Exogenous carbon monoxide suppresses LPS-induced platelet SNAREs complex assembly and α-granule exocytosis via integrin αIIbβ3-mediated PKCθ/Munc18a pathway"

“…Platelet α-granule distribution was observed by a transmission electron microscope (TEM) as previous described [55][56][57]. Briefly, samples were fixed by mixing them with an equal volume of 3% glutaraldehyde in 0.1 mol phosphate buffer for 1.5 hours at room temperature.…”