Platelet granules in vascular integrity

Meyer, Simon F. De

doi:10.1182/blood-2017-02-763540

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…Upon platelet activation, their highly organized cytoskeleton converts the disc-shaped platelets into hemisphere-shaped structures with extended filopodia [ 16 ]. Platelet activation consequently results in firm adhesion, aggregation and the formation of a hemostatic platelet plug [ 17 ]. Beside their role in hemostasis, they are also key players in inflammatory processes [ 15 ].…”

Section: Platelet-derived Factorsmentioning

confidence: 99%

Changes in Maternal Platelet Physiology during Gestation and Their Interaction with Trophoblasts

Forstner

Guettler

Gauster

2021

IJMS

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Upon activation, maternal platelets provide a source of proinflammatory mediators in the intervillous space of the placenta. Therefore, platelet-derived factors may interfere with different trophoblast subtypes of the developing human placenta and might cause altered hormone secretion and placental dysfunction later on in pregnancy. Increased platelet activation, and the subsequent occurrence of placental fibrinoid deposition, are linked to placenta pathologies such as preeclampsia. The composition and release of platelet-derived factors change over gestation and provide a potential source of predicting biomarkers for the developing fetus and the mother. This review indicates possible mechanisms of platelet-trophoblast interactions and discusses the effect of increased platelet activation on placenta development.

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Section: Platelet-derived Factorsmentioning

confidence: 99%

Changes in Maternal Platelet Physiology during Gestation and Their Interaction with Trophoblasts

Forstner

Guettler

Gauster

2021

IJMS

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“… 9 , 10 Active platelets secrete growth factors stored in their granules, which causes additional LEC and EC activation and proliferation, thus acting as a positive feedback loop (Aii). 9 , 58 Finally, platelets are heterogenous because of the mechanisms of their production: platelets are “ripped” from the megakaryocytes, and thus, platelet membrane receptor expression is not uniform. 54 Hereby, it can be expected that some of the platelets in the blood flow have higher CLEC-2 levels (green platelets on the scheme) and some have lesser CLEC-2 levels (white platelets on the scheme).…”

Section: Discussionmentioning

confidence: 99%

Platelet functional abnormalities in pediatric patients with kaposiform hemangioendothelioma/Kasabach-Merritt phenomenon

et al. 2023

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Kaposiform hemangioendothelioma (KHE) is a rare vascular tumor of infancy which is commonly associated with a life-threatening thrombocytopenic condition, Kasabach-Merritt phenomenon (KMP). Platelet CLEC-2 - tumor podoplanin interaction is considered as the key mechanism of platelet clearance in these patients. Here we aimed to assess platelet functionality in such patients. Three groups by 6-9 children were enrolled: a) with KHE/KMP without hematologic response to therapy (HR), b) with KHE/KMP with HR, and c) healthy children. Platelet functionality was assessed by continuous and endpoint flow cytometry, low-angle light scattering aggregometry (LaSca), fluorescent microscopy of blood smears and ex vivo thrombi formation. Platelet integrin activation in response to a combination of CRP (GPVI agonist) and TRAP-6 (PAR1 agonist), as well as calcium mobilization and integrin activation in response to CRP or rhodocytin (CLEC-2 agonist) alone, were significantly diminished in groups (a) and (b). At the same time platelet responses to ADP with or without TRAP-6 were unaltered. Thrombi formation from collagen in parallel plate flow chambers was also noticeably decreased in groups (a) and (b). In silico analysis of these results predicted diminished amounts of CLEC-2 on platelet surface of patients, which was further confirmed by immunofluorescence microscopy and flow cytometry. Additionally, we also noted a decrease in GPVI levels on platelets from group (a). In KHE/KMP platelet responses induced by CLEC-2 or GPVI activation are impaired due to diminished amount of receptors on platelet surface. This impairment correlates with the severity of the disease and resolves as patient recovers.

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“…There were controversial findings in this study as the RBC in the treatment groups 50mg/kg and 200mg/kg+NaCl were significantly lower than the neutral control group meanwhile no change was observed with the lone administration of the extract at 25, 100 and 200mg/kg of body weight/day. Platelets are known to prevent excessive posttraumatic blood loss at sites of vascular injury by forming a platelet plug [18]. The extract at the highest dose of 200mg/kg/day was able to prevent the drop of platelets caused by the lone administration of NaCl, this could be beneficial in the prevention of haemorrhage.…”

Section: Discussionmentioning

confidence: 99%

Kalanchoe Pinnata aqueous extract has the potential to improve haematological profile and fatigue status of mice subjected to Free Swimming Endurance Test

Bopda¹,

Maloba²,

Monju³

et al. 2021

GSC Biol. and Pharm. Sci.

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Introduction: Kalanchoe pinnata extracts have been shown to possess beneficial cardiovascular effects, analgesic and myorelaxant activities. This study aimed at evaluating the haematological and anti-fatigue effects of its aqueous extract. Materials and methods: This was an in vivo pharmacological experiment, carried out in the Laboratory of Zoology, University of Buea, from January to May 2018. A total of 48 mice were subjected to a 90mins/day-free endurance swimming test for 14 days. Two groups of mice received distilled water and 12% NaCl solution, respectively. Five groups received the plant extract (25, 50, 100 and 200 mg/kg/day) and 200mg/kg/day+NaCl. One positive control group received 5% Vitamin C (1.97mg/kg/day). All administrations were by gavage. Maximum swimming time, glycaemia, lactatemia, uremia, triglyceridemia, haematological indices, tissue glutathion peroxidase, malondialdehyde, glucose and creatine kinase-MB in the heart or gastrocnemius muscle were measured. Results: K. pinnata (50 and 100mg/kg/day) induced a non-significant increase of the swimming duration, compared to neutral control. However, the 12%NaCl group recorded a significant (P<0.05) increase. In the blood, NaCl induced a decrease of platelets that was significantly reverted by the extract in the 200mg/kg+NaCl group. The extract prevented the increase of the level of CK-MB in NaCl group and decreased the serum triglycerides, glucose, urea nitrogen and Lactate levels. In the tissues, MDA and CK-MB levels significantly increased (P<0.001) in the negative control. These increases were significantly (P<0.001) prevented by K. pinnata (200mg/kg/day). Conclusions: Significant biochemical variations support the hypothesis that K. pinnata could be used to delay exercise induced fatigue.

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Platelet granules in vascular integrity

Cited by 7 publications

References 10 publications

Changes in Maternal Platelet Physiology during Gestation and Their Interaction with Trophoblasts

Changes in Maternal Platelet Physiology during Gestation and Their Interaction with Trophoblasts

Platelet functional abnormalities in pediatric patients with kaposiform hemangioendothelioma/Kasabach-Merritt phenomenon

Kalanchoe Pinnata aqueous extract has the potential to improve haematological profile and fatigue status of mice subjected to Free Swimming Endurance Test

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