Platelet-Rich Concentrate Supports Human Mesenchymal Stem Cell Proliferation, Bone Morphogenetic Protein-2 Messenger RNA Expression, Alkaline Phosphatase Activity, and Bone Formation In Vitro: A Mode of Action to Enhance Bone Repair

Abstract

Taken together, the data presented here suggest that treatment of hMSC with clotted PRC, in an osteoinductive environment, enhances osteoblastic commitment and bone formation. Furthermore, these data indicate that the enhanced osteogenesis seen in the presence of PRC cannot be explained solely by enhanced cell proliferation, suggesting that PRC modulates a number of cell and molecular pathways to promote bone formation.

Role of autologous platelet-rich plasma in treatment of long-bone nonunions: a prospective study

“…Exogenous application of platelet-associated growth factors have been shown to stimulate proliferation of osteoblasts in culture [26,32] and has been demonstrated to stimulate the recruitment and proliferation of osteoblasts in defects in skulls of rabbits and to augment the healing of tibial fractures in rats in a dose-dependent manner [33,34]. More importantly, the application of autologous platelet-rich plasma used as source for growth factors has been demonstrated clinically in maxillofacial and mandibular defects as well as lumbar fusion with promising results [8,[35][36][37][38].…”

Autologous platelet rich plasma enhances satellite cells expression of MyoD and exerts angiogenic and antifibrotic effects in experimental rat model of traumatic skeletal muscle injury

“…These findings were in agreement with result of the present work. Furthermore, earlier in vitro studies confirmed that PRP stimulated the proliferation of a variety of human cell types including mesenchymal satellite cells (MSCs) derived from bone marrow [62,63] and adipose tissue [64] . [44] found better healing of muscle injuries in mice treated with autologous conditioned serum (a form of PRP) when compared to controls .There was a significant increase in centrally nucleated large myofibers indicating regeneration.…”

Interaction of Platelet-Rich Concentrate With Bone Graft Materials: An In Vitro Study

“…18 Briefly, 9 mL of priming solution (Smith & Nephew Inc) was injected into the device followed by 60 mL of anticoagulated blood and mixed. The blood plus priming solution mixture was then allowed to flow through the filter.…”

“…16 Our recent data may also shed some light on the interaction between PRC and DBM; these data demonstrated that when combined, there was altered release kinetics of growth factors from the composite clot compared with PRC alone. 18 Our central hypothesis is that PRC promotes tissue healing through the early activation of cellular responses such as angiogenesis, progenitor cell recruitment, and cell proliferation. 19,20 We propose that cell fate determination is dependent on additional factors derived either from the microenvironment or from the additional graft materials.…”