Platelet-Rich fibrin: A “wonder material” in advanced surgical dentistry

Maniyar, Nikunj; Sarode, Gargi S; Sarode, Sachin C.; Shah, Jahanvi

doi:10.4103/mjdrdypu.mjdrdypu_204_17

Cited by 14 publications

(11 citation statements)

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“…One of the latest innovations in in vivo tissue engineering applications is the use of platelet concentrates. Two types of such platelet concentrates are available, Platelet-Rich Plasma (PRP) and PRF [12]. Choukroun in the early 2000s focused at developing a second generation platelet concentrate without using anticoagulation factors [4].…”

Section: Resultsmentioning

confidence: 99%

TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

et al. 2020

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Objective: Modification of the speed and time of centrifugation based on the low-speed centrifugation concept for platelet-rich fibrin (PRF) has resulted in a new type of PRF known as advanced PRF (A-PRF). A-PRF can release several types of growth factors (GFs) that participate in the process of differentiation, such as transforming GF-β1 (TGF-β1). The aim of this study was to analyze TGF-β1 expression in various concentrations of A-PRF in the differentiation process of human dental pulp stem cells (hDPSCs).Methods: hDPSC cultures were obtained from those of previous research (ethical approval form has been attached). These hDPSCs were in the 2nd–3rd passage, and serum starvation was done by reducing fetal bovine serum (FBS) levels in the hDPSC culture media. A-PRF was obtained using 10 ml blood collected from the cubital vein, which was centrifuged at 1500 rpm for 14 min and then divided into four concentration groups. TGF-β1 expression in 1%, 5%, and 25% A-PRF as well as in 10% FBS (control) was analyzed by ELISA on day 7.Results: Although no significant differences were observed in TGF-β1 expression between 1%, 5%, and 25% A-PRF, and 10% FBS, it was observed that the higher the concentration of A-PRF, the greater the TGF-β1 expression.Conclusion: The expression of TGF-β1 was consistent with the increase in A-PRF concentration. The highest TGF-β1 expression was detected in 25% A-PRF among all concentrations in the differentiation process of hDPSCs.

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Section: Resultsmentioning

confidence: 99%

TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

et al. 2020

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“…The PRF can modulate the healing of soft and hard tissues through gradual and prolonged release of growth factors, guaranteeing the homeostasis and stimulating the angiogenesis and cellular proliferation [ 49 , 50 ]. The acceleration of the wound healing based on PRF is promoted by increasing the wound site of the growth factors, then transforming β (TGF-β), insulin-like (IGF), platelet-derived (PDGF), vascular endothelial (VEGF), fibroblastic (FGF), and epithelial (EGF) [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Autologous Matrix of Platelet-Rich Fibrin in Wound Care Settings: A Systematic Review of Randomized Clinical Trials

Carvalho

Fernandes

Souza

2020

JFB

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Platelet-rich fibrin (PRF) consists of a matrix that provides the necessary elements for wound healing, acting as a biodegradable scaffold for cell migration, proliferation, and differentiation, in addition to the delivery of growth factors and angiogenesis. This study aims to determine the effectiveness of the autologous PRF in the treatment of wounds of different etiologies. We carried out a systematic review of randomized clinical trials, guided by the recommendations of the Cochrane Collaboration using the following databases: Pubmed/MEDLINE, EMBASE, Web of Science, and CENTRAL. The search strategy resulted in the inclusion of ten studies that evaluated the use of PRF dressings for the healing of acute or chronic wounds of multiple etiologies. Among the 172 participants treated with PRF in wounds of varying etiologies and different segment times, 130 presented favorable events with the use of the intervention. Among the 10 studies included, only two of them did not demonstrate better results than the control group. The studies showed clinical heterogeneity, making it impossible to perform a meta-analysis. The findings do not provide enough evidence to support the routine use of PRF dressings as the first line of treatment for the healing of acute or chronic wounds of different etiologies. There was great variability in the application of the various protocols and the ways to prepare the PRF, resulting in clinical heterogeneity. Therefore, it makes it impossible to synthesize and to collect evidence from different types of studies in the meta-analysis, which affects the results and their proper discussion.

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“…Neoangiogenesis is promoted by human osteoblasts which is due to PRF. 7 PRF acts as a matrix to promote the healing of wound edges when used as a fibrin bandage in a membrane shape. The preparation of the chairside is quite inexpensive, easy and the process is simplified and fast.…”

Section: Discussionmentioning

confidence: 99%

“…The resultant amount is very less as it is autologous blood. 7 Advantages of PRF: PRF helps in soft tissue repair that protects the surgical site and accelerates wound healing, when bone graft is mixed to PRF it acts as a biological connector. Osteoprogenitor cells and stem cells are attracted to the centre of the graft.…”

Section: Prf -Platelet-rich Fibrinmentioning

confidence: 99%

Platelet Rich Fibrin in Socket Preservation

Monalisha Mohapatra

2020

Indian Journal of Forensic Medicine & Toxicology

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With the advancement in dentistry, there are different ways of treatment such as restoration, surgical procedures as well as aesthetic treatments socket healing after extraction comprises of hard tissue as well as soft tissue reconstruction with changes which is maximum dimensionally during the initial three months. One of the major challenges in clinical science is the development of bioactive surgical additives to improve the pace of the process of healing and inflammation control. Platelet-rich fibrin (PRF) exists in this context as a safe and adequate substitute with less risk and favourable outcomes. The methodology of use of PRF, concentration on its planning advantages and drawbacks of use in clinical applications are summarized in the following review. Platelet-rich fibrin (PRF) was developed in France first as a beneficial substitute to platelet-rich plasma to overcome its limitations.

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Platelet-Rich fibrin: A “wonder material” in advanced surgical dentistry

Cited by 14 publications

References 1 publication

TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

TRANSFORMING GROWTH FACTOR-β1 EXPRESSION IN VARIOUS CONCENTRATIONS OF ADVANCED PLATELET-RICH FIBRIN MODULATING HUMAN DENTAL PULP STEM CELL DIFFERENTIATION

Autologous Matrix of Platelet-Rich Fibrin in Wound Care Settings: A Systematic Review of Randomized Clinical Trials

Platelet Rich Fibrin in Socket Preservation

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