Platelet-rich fibrin (PRF) in implant dentistry in combination with new bone regenerative technique in elderly patients

Cortese, Antonio; Pantaleo, Giuseppe; Borri, Antonio; Caggiano, Mario; Amato, Massimo

doi:10.1016/j.ijscr.2016.09.022

Cited by 56 publications

(40 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TGF-β in PRF stimulates progenitor cell proliferation and differentiation with osteoblastic mitogenesis. [ 19 24 25 ] Yang et al . argued that PRF significantly increased BALP expression and mRNA levels of osteogenic marker that reduces adipogenic capability.…”

Section: Discussionmentioning

confidence: 99%

“…PRF contains TGF-β1 that plays an important role in stimulating fibrosis, PDGF contributes to progenitor cell migration and survival, IGF prevents cells from premature apoptosis, and VEGF stimulates vasculogenesis and angiogenesis, while EGF is active during cell proliferation and differentiation. [ 19 25 ] PRF can increase the expression of protein kinase and osteoprotegerin in osteoblasts that can stimulate osteoblast proliferation indicating the potential to form new bone formation. PRF is also capable of inducing MSCs to differentiate into in vitro osteoblasts such as BMSCs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bone alkaline phosphatase and osteocalcin expression of rat's Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro study)

et al. 2018

View full text Add to dashboard Cite

Objective:The aim of this study was to analyze the osteogenic differentiation of rat GMSCs cultured in PRF for bone remodeling.Materials and Methods:GMSCs were isolated from the lower gingival tissue of four healthy, 250 g, 1-month old, male rats (Rattus norvegicus) cut into small fragments, cultured for 2 weeks, and subsequently passaged every 4–5 days. GMSCs isolated in passage 3 were characterized by CD34, CD45, CD44, CD73, CD90, and CD105 using fluorescein isothiocyanate immunocytochemistry (ICC) examination. GMSCs in passage 3–5 cultured in five M24 plates (N = 108; n = 6/group) for 7, 14, and 21 days with three different mediums as follows: Control (−) group: α-Modified Eagle Medium; Control (+) group: High-dose glucose Dulbecco's Modified Eagle's Medium (DMEM-HG) + osteogenic medium; and treatment group: DMEM-HG + osteogenic medium + PRF. GMSCs were osteogenic differentiation cultured in vitro in three different mediums by bone alkaline phosphatase (BALP) and osteocalcin (OSC) marker using ICC monoclonal antibody.Statistical Analysis Used:The one-way analysis of variance was performed (P < 0.05) based on Shapiro–Wilk and Levene's tests (P > 0.05).Results:GMSCs were shown to present + CD44, +CD73, +CD90, +CD105 and − CD34, − and CD45 expression as MSCs markers. The treatment group showed the highest BALP expression (16.00 ± 1.732) on day 7, while OSC expression (13.67 ± 2.309) on day 21 showed the statistically significant difference between groups (P < 0.05).Conclusion:GMSCs cultured in PRF demonstrated potential osteogenic differentiation ability capable of accelerating in vitro bone remodeling by enhancing BALP and OSC expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bone alkaline phosphatase and osteocalcin expression of rat's Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro study)

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Allografts and xenografts are osteoconductive but not osteoinductive [4,5], unlike other xenograft equine-derived biomaterials. They are used successfully in several fields of oral surgery [6][7][8][9][10][11][12][13][14] and should be preferred for being resistant to prion infections (i.e., Bovine Spongiform Encephalopathies) [15,16].…”

Section: Introductionmentioning

confidence: 99%

Comparative Investigation of Cutting Devices on Bone Blocks: An SEM Morphological Analysis

et al. 2019

View full text Add to dashboard Cite

Background: Bone regeneration is a reliable technique when the bone volume is insufficient to provide a functional and aesthetic outcome in surgery and implantoprosthesis procedures. When bone blocks are used but do not match the shape of the defect, the block must be adapted. The aim of our research was to evaluate, by Scanning Electron Microscopy (SEM) morphological observation, how different cutting devices modify the bone surface. Method: Four equine bone blocks were divided into 15 cubic shape samples with ultrasonic and sonic tips, as well as diamond, tungsten carbide, and Lindemann burs. The uncut surface of the obtained bone block was used as a control. Two observers independently analyzed the SEM observation recording, including cut precision, depth of incision, thermal damages, and presence of bone debris. For each group, sharpness, depth, carbonization, and bone debris were expressed as mean values. Results: The osteotomy performed with an ultrasonic tip shows the best results, preserving the bone morphology in both quantitative and qualitative analyses. The bone surface appeared sufficiently clean from debris and showed a reduced presence of carbonization. Conclusion: The shaping of the bone block as in vivo osteotomy respects the bone morphology and allows it to achieve the relevant biological and clinical outcome.

show abstract

“…To manage this situation, the split crest technique was proposed in the first original study by Osborn in 1985 for alveolar bone expansion and implant insertion in one stage surgery without the need for an autologous bone graft [15-16]. …”

Section: Introductionmentioning

confidence: 99%

Platelet-rich fibrin (PRF) in implants dentistry in combination with new bone regenerative flapless technique: evolution of the technique and final results

et al. 2017

Self Cite

View full text Add to dashboard Cite

AbstractMost common techniques for alveolar bone augmentation are guided bone regeneration (GBR) and autologous bone grafting. GBR studies demonstrated long-term reabsorption using heterologous bone graft. A general consensus has been achieved in implant surgery for a minimal amount of 2 mm of healthy bone around the implant.A current height loss of about 3-4 mm will result in proper deeper implant insertion when alveolar bone expansion is not planned because of the dome shape of the alveolar crest. To manage this situation a split crest technique has been proposed for alveolar bone expansion and the implants’ insertion in one stage surgery. Platelet-rich fibrin (PRF) is a healing biomaterial with a great potential for bone and soft tissue regeneration without inflammatory reactions, and may be used alone or in combination with bone grafts, promoting hemostasis, bone growth, and maturation.AimThe aim of this study was to demonstrate the clinical effectiveness of PRF combined with a new split crest flapless modified technique in 5 patients vs. 5 control patients.Materials and methodsTen patients with horizontal alveolar crests deficiency were treated in this study, divided into 2 groups: Group 1 (test) of 5 patients treated by the flapless split crest new procedure; Group 2 (control) of 5 patients treated by traditional technique with deeper insertion of smaller implants without split crest. The follow-up was performed with x-ray orthopantomography and intraoral radiographs at T0 (before surgery), T1 (operation time), T2 (3 months) and T3 (6 months) post-operation.ResultsAll cases were successful; there were no problems at surgery and post-operative times. All implants succeeded osteointegration and all patients underwent uneventful prosthetic rehabilitation. Mean height bone loss was 1 mm, measured as bone-implant most coronal contact (Δ-BIC), and occurred at immediate T2 post-operative time (3 months). No alveolar bone height loss was detected at implant insertion time, which was instead identified in the control group because of deeper implant insertion.ConclusionThis modified split crest technique combined with PRF appears to be reliable, safe, and to improve the clinical outcome of patients with horizontal alveolar crests deficiency compared to traditional implanting techniques by avoiding alveolar height-loss related to deeper insertion of smaller implants.

show abstract

Platelet-rich fibrin (PRF) in implant dentistry in combination with new bone regenerative technique in elderly patients

Cited by 56 publications

References 26 publications

Bone alkaline phosphatase and osteocalcin expression of rat's Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro study)

Bone alkaline phosphatase and osteocalcin expression of rat's Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro study)

Comparative Investigation of Cutting Devices on Bone Blocks: An SEM Morphological Analysis

Platelet-rich fibrin (PRF) in implants dentistry in combination with new bone regenerative flapless technique: evolution of the technique and final results

Contact Info

Product

Resources

About