Platelet-rich plasma as an additional therapeutic option for infected wounds with multi-drug resistant bacteria: in vitro antibacterial activity study

Çeti̇nkaya, Rıza Aytaç; Yeni̇lmez, Ercan; Petrone, Patrizio; Yılmaz, Sercan; Bektöre, Bayhan; Şimsek, Berksan; Ati̇k, Tuğba Kula; Özyurt, Mustafa; Ünlü, Aytekin

doi:10.1007/s00068-018-0957-0

Cited by 33 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As expected, growth at RT was significantly greater than that observed in CS samples, with growth in CS samples largely static throughout. Interestingly, despite reports of antimicrobial activity associated with PLT-rich plasma, [19][20][21][22] no obvious antimicrobial activity was observed in either RT or CS samples. Because metabolic activity is reduced with refrigeration, we sought to determine if metabolic by-products may contribute to bacterial growth as well.…”

Section: Bacterial Growthmentioning

confidence: 82%

Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

et al. 2019

View full text Add to dashboard Cite

INTRODUCTION Due to high risk of septic transfusion reactions arising from bacterial contamination, US Food and Drug Administration regulations currently limit platelet storage to 5 days at room temperature (RT). However, blood culturing methods can take up to 7 days to detect bacteria, allowing transfusion of potentially contaminated units. Thus, cold storage (CS) may be a viable means of extending shelf life and improving safety. STUDY DESIGN AND METHODS Platelets and fresh plasma (FP) were collected by apheresis from healthy donors, aliquoted, and challenged with Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, or Staphylococcus epidermidis. Aliquots were then stored at either RT or CS. RESULTS Significant (p < 0.05) bacterial growth was detected at RT for most bacteria as early as Day 1 after collection, with peak growth occurring between Days 3 and 4. Growth remained static during CS. Additionally, platelets appeared to enhance bacterial replication with growth significantly lower (p < 0.05) in FP relative to RT‐stored platelets. Lactic acid promoted bacterial growth when added to FP at RT. Bacterial challenge also resulted in significantly increased platelet activation (p < 0.05) and significantly reduced platelet function (p < 0.05) in RT storage relative to uninfected controls by Day 5 after collection. Conversely, CS ablated bacteria growth, limited platelet metabolism, and preserved platelet function throughout the study. CONCLUSION These data suggest that CS presents an attractive alternative to RT to both extend storage life and reduce the risk of transfusion‐related sepsis.

show abstract

Section: Bacterial Growthmentioning

confidence: 82%

Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Essentially, upon calcium and/or thrombin addition, PRP forms a platelet-rich fibrin scaffold (PRF) that can be layered in the wound bed. PRF degradation is highly regulated by the serine protease system from plasma PAI-1, PAI-2 (plasminogen activator inhibitor type 1 and 2), TAF1 (TATA-box binding protein, associated factor), plasmin [12,13] and can be synchronized with the healing process.…”

Section: Introductionmentioning

confidence: 99%

Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care

Amo

Perez‐Valle

Pérez-Zabala

et al. 2020

IJMS

View full text Add to dashboard Cite

The use of platelet-rich fibrin (PRF) is investigated in ulcer management because it provides a healing milieu rich in growth factors and cytokines. Although crucial, the relevance of secondary dressings is under-researched and no data support the use of any particular dressing in preference to another. We assessed the properties of different dressing categories, including alginates, hydrocolloids, foams, hydrofibers, films, meshes and gauzes, in terms of affinity for PRF, releasate management (retention/extrusion) and the kinetics of cytokine release as well as the influence of each combination product, [PRF + dressing], on dermal cell behaviour, aiming to provide useful information for choosing the most adequate dressing for each particular patient. Active dressings including alginates, hydrofibers, foams and hydrocolloids blend with PRF, creating a diverse combination of products with different performances. Alginate and hydrofiber showed the highest affinity but moderate retention of releasate, without interfering with cell functions. Instead, the foam sequestered the releasate and hindered the release of growth factors, thereby compromising cell activities. Film and mesh presented very poor releasate retention and performed similarly to PRF by itself. Affinity index and releasate management explained 79% of platelet-derived growth factor (PDGF-BB) concentration variability, p < 0.001. Cell proliferation depended on the ability of the combination product to retain/release supernatant, PDGF-BB concentration and cell adhesion R 2 = 0.91, p = 0.014.

show abstract

“…In rats with spinal cord injury, PRP can also enhance the locomotor recovery and spared white matter, promote angiogenesis and neuronal regeneration . In vitro study also revealed that PRP can significantly improve infected wound healing against drug resistant bacteria . Through increase of vascular endogenous growth factor, PRP is effective in treatment of avascular necrosis of femoral head in rabbits .…”

Section: Discussionmentioning

confidence: 97%

“…Patients with a GRA ≥ 2 had a significant longer therapeutic duration compared to those with a GRA < 2 wound healing against drug resistant bacteria. 23 Through increase of vascular endogenous growth factor, PRP is effective in treatment of avascular necrosis of femoral head in rabbits. 24 Put together, activated PRP can induce a local inflammation which overrides the unsolved residual inflammatory process and promote wound healing and tissue regeneration.…”

Section: Figurementioning

confidence: 99%

Intravesical injections of platelet‐rich plasma is effective and safe in treatment of interstitial cystitis refractory to conventional treatment—A prospective clinical trial

Jhang

Lin

Kuo

2018

Neurourology and Urodynamics

View full text Add to dashboard Cite

Aims Current treatments for interstitial cystitis/bladder pain syndrome (IC/BPS) are usually unsuccessful in achieving long‐term bladder pain relief and irritable symptom improvement. This study investigated the clinical efficacy of platelet‐rich plasma (PRP) intravesical injections on IC/BPS patients refractory to conventional therapies. Methods Forty patients received four monthly intravesical injections of 10 mL PRP extracted from 50 mL of whole blood. The primary end‐point was Global Response Assessment (GRA) at 3 months after the 4th PRP injection. Secondary endpoints included changes in O'Leary‐Sant symptom score (OSS), visual analog scale (VAS) of pain, daily frequency, nocturia, functional bladder capacity (FBC), maximum flow rate, voided volume, post‐void residual volume (PVR) from baseline to 3 months after the 4th PRP injection. Results All 40 patients (37 women and 3 men, aged 55.5 ± 11.1 years) completed the four injections and follow‐up visits. GRA improved after the 1st PRP injection and the satisfaction persists till the primary end‐point. The success rate was 45%, 52%, 70%, 70%, and 67.5% after the 1st, 2nd, 3rd, 4th, and 3 months after the 4th PRP injection, respectively. OSS and VAS also significantly decreased. The PVR did not change after repeated PRP injections, FBC increased, frequency, and nocturia were decreased after PRP injections. All patients were free of urinary tract infection or difficulty urinating. Conclusion The study demonstrated that repeated intravesical injections of autologous PRP can increase bladder capacity and provide IC symptom improvement in patients with IC/BPS refractory to conventional therapy. Autologous PRP injection is safe and effective in selected patients.

show abstract

Platelet-rich plasma as an additional therapeutic option for infected wounds with multi-drug resistant bacteria: in vitro antibacterial activity study

Abstract: Emerging PRP and other platelet-derived products seem to be promising alternative tools besides antibiotic treatment, debridement, negative pressure wound therapy, hyperbaric oxygen therapy, and other treatment options for treating diabetic foot infections.

Cited by 33 publications

References 31 publications

Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

Platelet enhancement of bacterial growth during room temperature storage: mitigation through refrigeration

Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care

Intravesical injections of platelet‐rich plasma is effective and safe in treatment of interstitial cystitis refractory to conventional treatment—A prospective clinical trial

Contact Info

Product

Resources

About