Platelet-rich plasma: evidence to support its use

Marx, Robert E.

doi:10.1016/j.joms.2003.12.003

Cited by 1,749 publications

(1,644 citation statements)

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“…It is known that platelets are a source of several growth factors, among which are; PDGF [1][2][3][4] , TGF-b 5,6 , VEGF 7,8 , IGF-I 4,9 and EGF 6,10 . This fact stimulated the development of a platelet concentrate with the intention of increasing the levels of local growth factors delivery, which, theoretically, if present at a damaged site, could improve the healing process.…”

Section: Introductionmentioning

confidence: 99%

“…Several authors started to use the platelet-rich plasma (PRP), mainly in association with autogenous bone graftings 4,7,9,12 , and also to improve soft tissue repair 4,10 .…”

Section: Introductionmentioning

confidence: 99%

“…In PRP experimentation there is a concern about the employed method of concentration regarding the ability to really concentrate platelets. It is also important not to damage the platelets during the process 10 . Thus, more studies in vivo and in vitro may contribute to clarify aspects of PRP use and its effectiveness.…”

Section: Introductionmentioning

confidence: 99%

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Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

et al. 2010

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PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

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Section: Introductionmentioning

confidence: 99%

“…Several authors started to use the platelet-rich plasma (PRP), mainly in association with autogenous bone graftings 4,7,9,12 , and also to improve soft tissue repair 4,10 .…”

Section: Introductionmentioning

confidence: 99%

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Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

et al. 2010

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“…Gel platelet-rich plasma (PRP) is also described as tissue glues with further advantage over fibrin glue, as it has growth factors and cytokines, providing great benefit in wound healing, such as better re-epitelization, reduction of wound exudate and necrosis (Bhanot 2002, Marx 2004, Vendramin et al 2010. Research related to improve platelet-rich plasma is very important, and numerous studies are being developed to make new discoveries about this blood by product.…”

Section: Introductionmentioning

confidence: 99%

Method to obtain platelet-rich plasma from rabbits (Oryctolagus cuniculus )

et al. 2016

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RESUMO.-[Método de obtenção de plasma rico em plaquetas de coelhos (Oryctolagus cuniculus).] O plasma rico em plaquetas (PRP) é um produto de fácil obtenção a baixo custo, destacando-se pelos seus fatores de crescimento na reparação tecidual. Para obtenção do PRP, a centrifugação do sangue total é realizada com tempos e forças gravitacionais específicas. Assim, o presente trabalho teve por objetivo estudar o método da dupla centrifugação para obtenção do PRP, a fim de avaliar a eficácia de aumento daMethod to obtain platelet-rich plasma from rabbits (Oryctolagus cuniculus)¹ Platelet-rich plasma (PRP) is a product easy and inxpesnsive, and stands out to for its growth factors in tissue repair. To obtain PRP, centrifugation of whole blood is made with specific time and gravitational forces. Thus, the present work aimed to study a method of double centrifugation to obtain PRP in order to evaluate the effective increase of platelet concentration in the final product, the preparation of PRP gel, and to optimize preparation time of the final sample. Fifteen female White New Zealand rabbits underwent blood sampling for the preparation of PRP. Samples were separated in two sterile tubes containing sodium citrate. Tubes were submitted to the double centrifugation protocol, with lid closed and 1600 revolutions per minute (rpm) for 10 minutes, resulting in the separation of red blood cells, plasma with platelets and leucocytes. After were opened and plasma was pipetted and transferred into another sterile tube. Plasma was centrifuged again at 2000rpm for 10 minutes; as a result it was split into two parts: on the top, consisting of platelet-poor plasma (PPP) and at the bottom of the platelet button. Part of the PPP was discarded so that only 1ml remained in the tube along with the platelet button. This material was gently agitated to promote platelets resuspension and activated when added 0.3ml of calcium gluconate, resulting in PRP gel. Double centrifugation protocol was able to make platelet concentration 3 times higher in relation to the initial blood sample. The volume of calcium gluconate used for platelet activation was 0.3ml, and was sufficient to coagulate the sample. Coagulation time ranged from 8 to 20 minutes, with an average of 17.6 minutes. Therefore, time of blood centrifugation until to obtain PRP gel took only 40 minutes. It was concluded that PRP was successfully obtained by double centrifugation protocol, which is able to increase the platelet concentration in the sample compared with whole blood, allowing its use in surgical procedures. Furthermore, the preparation time is appropriate to obtain PRP in just 40 minutes, and calcium gluconate is able to promote the activation of platelets.INDEX TERMS: Platelet-rich plasma, platelet concentration, revascularization, rabbits.

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“…Os FCs podem ter ação parácrina, endócrina ou autócrina, e seu estímulo é transmitido via receptores de superfície de membrana, que ativam proteínas reguladoras no citoplasma, gerando respostas através da indução da expressão de genes (MARX, 2004 O objetivo desta revisão é descrever a função, as técnicas de obtenção, a utilização e o mecanismo de ação das CMIs e dos FCs como recentes tecnologias utilizadas em enxerto ósseo.…”

Section: Introductionunclassified