Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage

Moussa, Mayssam; Lajeunesse, Daniel; Hilal, George; Atat, Oula El; Haykal, Gaby; Serhal, Rim; Chalhoub, Antonio; Khalil, Charbel; Alaaeddine, Nada

doi:10.1016/j.yexcr.2017.02.012

Cited by 182 publications

(158 citation statements)

References 53 publications

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“…A preliminarily study on apoptosis in ACs and CPCs was performed by the detection of cleaved caspase-3, which represents a reliable marker for cells that are dying by apoptosis [39] in immunofluorescence experiments ( Figure S1). Contrary to Moussa et al [38], we did not detect any evident difference in cleaved caspase-3 expression between the three examined experimental groups; rather, its levels appeared quite low. Furthermore, it could be necessary to induce stress or apoptotic signals in order to trigger and subsequently detect an appreciable anti-apoptotic effect of PL.…”

Section: Pl Increased the Proliferation Of Acs And Reduced Their Senecontrasting

confidence: 99%

“…Representative images of the three experimental cell groups after staining and the percentage of positive cells are shown in Figure 2C. Among the beneficial effects of platelet derivatives, chondroprotection could probably also occur by reducing the apoptosis rate and a recent study seems to confirm this tendency [38]. A preliminarily study on apoptosis in ACs and CPCs was performed by the detection of cleaved caspase-3, which represents a reliable marker for cells that are dying by apoptosis [39] in immunofluorescence experiments ( Figure S1).…”

Section: Pl Increased the Proliferation Of Acs And Reduced Their Senementioning

confidence: 85%

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Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies

Carluccio

Martinelli

Palamà

et al. 2020

Cells

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Regenerative strategies for human articular cartilage are still challenging despite the presence of resident progenitor cell population. Today, many efforts in the field of regenerative medicine focus on the use of platelet derivatives due to their ability to reactivate endogenous mechanisms supporting tissue repair. While their use in orthopedics continues, mechanisms of action and efficacy need further characterization. We describe that the platelet lysate (PL) is able to activate chondro-progenitor cells in a terminally differentiated cartilage tissue. Primary cultures of human articular chondrocytes (ACs) and cartilage explants were set up from donor hip joint biopsies and were treated in vitro with PL. PL recruited a chondro-progenitors (CPCs)-enriched population from ex vivo cartilage culture, that showed high proliferation rate, clonogenicity and nestin expression. CPCs were positive for in vitro tri-lineage differentiation and formed hyaline cartilage-like tissue in vivo without hypertrophic fate. Moreover, the secretory profile of CPCs was analyzed, together with their migratory capabilities. Some CPC-features were also induced in PL-treated ACs compared to fetal bovine serum (FBS)-control ACs. PL treatment of human articular cartilage activates a stem cell niche responsive to injury. These facts can improve the PL therapeutic efficacy in cartilage applications.

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Section: Pl Increased the Proliferation Of Acs And Reduced Their Senecontrasting

confidence: 99%

Section: Pl Increased the Proliferation Of Acs And Reduced Their Senementioning

confidence: 85%

Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies

Carluccio

Martinelli

Palamà

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…The anti-inflammatory effect of PRP is well confirmed by previous studies [42, 43]. In addition, PRP cleavage products were reported to be able to terminate the NF- κ B pathway and downregulate the production of COX-2 [44].…”

Section: Discussionsupporting

confidence: 61%

The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Intervertebral Disc Degeneration

Jia

Wang

et al. 2018

Stem Cells International

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Background Platelet-rich plasma (PRP) is a promising strategy for intervertebral disc degeneration. However, the potential harmful effects of leukocytes in PRP on nucleus pulposus-derived mesenchymal stem cells (NPMSCs) have seldom been studied. This study aimed at comparatively evaluating effects of pure platelet-rich plasma (P-PRP) and leukocyte-containing platelet-rich plasma (L-PRP) on rabbit NPMSCs in vitro. Methods NPMSCs isolated from rabbit NP tissues were treated with L-PRP or P-PRP in vitro, and then cell proliferation and expression of stem cell markers, proinflammatory cytokines (TNF-α, IL-1β), production of ECM (extracellular matrix-related protein), and NF-κB p65 protein were validated by CCK-8 assay, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, immunofluorescence, and western blot respectively. Results NPMSCs differentiate into nucleus pulposus-like cells after treatment of PRPs (P-PRP and L-PRP), and NPMSCs exhibited maximum proliferation at a 10% PRP dose. L-PRP had observably higher concentration of leukocytes, TNF-α, and IL-1β than P-PRP. Furthermore, compared to P-PRP, L-PRP induced the differentiated NPMSCs to upregulate the expression of TNF-α and IL-1β, enhanced activation of the NF-κB pathway, increased the expression of MMP-1 and MMP-13, and produced less ECM in differentiated NPMSCs. Conclusions Both P-PRP and L-PRP can induce the proliferation and NP-differentiation of NPMSCs. Compared to L-PRP, P-PRP can avoid the activation of the NF-κB pathway, thus reducing the inflammatory and catabolic responses.

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“…The pathophysiology of OA is a complex, multi‐factorial process that involves the interplay between mechanics, biology, and structure . Biological factors are thought to include increasing chondrocyte insensitivity to anabolic signals and an increasingly inflammatory joint milieu . The mainstay of treatment in OA has long included anti‐inflammatory therapy for symptomatic relief until end‐stage joint destruction which can be treated with joint replacement.…”

mentioning

confidence: 99%

Platelet‐Rich Plasma (PRP) From Older Males With Knee Osteoarthritis Depresses Chondrocyte Metabolism and Upregulates Inflammation

O’Donnell

Migliore

Grandi

et al. 2019

Journal Orthopaedic Research

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There is intense clinical interest in the potential effects of platelet-rich plasma (PRP) for the treatment of osteoarthritis (OA). This study tested the hypotheses that (i) "lower" levels of the inflammatory mediators (IMs), interleukin-1β, and tumor necrosis factor α (TNF-α) and (ii) "higher" levels of the growth factors (GFs), insulin-like growth factor 1, and transforming growth factor β1 within leukocyte-poor PRP correlate with more favorable chondrocyte and macrophage responses in vitro. Samples were collected from 10 "healthy" young male (23-33 years old) human subjects (H-PRP) and nine older (62-85 years old) male patients with severe knee OA (OA-PRP). The samples were separated into groups of "high" or "low" levels of IM and GF based on multiplex cytokine and enzyme-linked immunosorbent assay data. Three-dimensional (3D) alginate bead chondrocyte cultures and monocyte-derived macrophage cultures were treated with 10% PRP from donors in different groups. Gene expression was analyzed by quantitative polymerase chain reaction. Contrary to our hypotheses, the effect of PRP on chondrocytes and macrophages was mainly influenced by the age and disease status of the PRP donor as opposed to the IM or GF groupings. While H-PRP showed similar effects on expression of chondrogenic markers (Col2a1 and Sox9) as the negative control group (p > 0.05), OA-PRP decreased chondrocyte expression of Col2a1 and Sox-9 messenger RNA by 40% and 30%, respectively (Col2a1, p = 0.015; Sox9, p = 0.037). OA-PRP also upregulated TNF-α and matrix metallopeptidase 9 (p < 0.001) gene expression in macrophages while H-PRP did not. This data suggests that PRP from older individuals with OA contain factors that may suppress chondrocyte matrix synthesis and promote macrophage inflammation in vitro.

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Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage

Abstract: These results suggest that PRP could be a potential therapeutic tool for the treatment of OA.

Cited by 182 publications

References 53 publications

Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies

Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies

The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Intervertebral Disc Degeneration

Platelet‐Rich Plasma (PRP) From Older Males With Knee Osteoarthritis Depresses Chondrocyte Metabolism and Upregulates Inflammation

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