Platinum diethylenetriaminepentaacetates

Grevtsev, A. M.; Zheligovskaya, N. N.; Popov, L. V.; Spitsyn, V. I.

doi:10.1007/bf01153251

Cited by 1 publication

(5 citation statements)

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“…It was our hope that DOTA, with its cyclic structure, would form a 1:1 complex with palladium that involved chelation only through the four tertiary amines. This result is desirable because palladium, being a soft metal, forms stronger bonds with amines than with carboxylic acids. , While not directly comparable, palladium forms a 1:1 complex with 1,4,8,11-tetrathiacyclotetradecane, a cyclic thioether . To our knowledge, there are no reports of palladium or platinum complexes with DOTA.…”

Section: Resultsmentioning

confidence: 98%

“…In the literature, the mechanism of complex formation between polyaminocarboxylates and K 2 PdCl 4 (or K 2 PtCl 4 ) is normally described as a two-step process. , In the first step, tertiary amino groups displace chloride ligands. For example, EDTA would form a bidentate complex with the metal center, whereas DTPA would form a tridentate complex.…”

Section: Resultsmentioning

confidence: 99%

“…In the second step, the remaining chloride ligands are removed through (a) the intentional addition of Ag + ions or (b) the reprecipitation of the complex in a solution that does not contain chloride ion. These methods allow carboxylic acid group(s) of the ligand to chelate the tetracoordinate, square-planar metal center as well. , In this work, we expect that the polymers initially chelate palladium through tertiary amines. After dissolution in phosphate buffer, the spin filter washes with phosphate buffer and water will remove the remaining chloride ligands in a process analogous to reprecipitation in a solution that does not contain chloride ion.…”

Section: Resultsmentioning

confidence: 99%

“…This result is desirable because palladium, being a soft metal, forms stronger bonds with amines than with carboxylic acids. 4,6 While not directly comparable, palladium forms a 1:1 complex with 1,4,8,11-tetrathiacyclotetradecane, a cyclic thioether. 21 To our knowledge, there are no reports of palladium or platinum complexes with DOTA.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…These 8 isotopes represent additional labels that can be used, in principle, to increase the multiplicity of immunoassays. In addition, there exists literature describing palladium(II) and platinum(II) complexes with polyaminocarboxylates such as EDTA, DTPA, and TTHA. − One of the challenges we address in this paper is the extension of our synthetic methodology to attach EDTA (ethylenediaminetetraacetic acid), TTHA (triethylenetetraminehexaacetic acid), and DOTA (tetraazocyclododecanetetraacetic acid) as pendant groups to the polymer backbone. Another more serious challenge is that many of the conditions used to synthesize polyaminocarboxylates complexes of Pd(II) and Pt(II) are quite harsh and risk destroying the polymeric maleimide group used for attachment to an antibody.…”

Section: Introductionmentioning

confidence: 99%

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Curious Results with Palladium- and Platinum-Carrying Polymers in Mass Cytometry Bioassays and an Unexpected Application as a Dead Cell Stain

et al. 2011

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We describe the synthesis of metal-chelating polymers (MCPs) with four different pendant polyaminocarboxylate ligands (EDTA, DTPA, TTHA, DOTA) and an orthogonal end-group, either a fluorescein molecule or a bismaleimide linker for antibody attachment. Polymer characterization by a combination of (1)H NMR, UV/vis absorption measurements, and thermal gravimetric analysis (TGA) indicated that each chain of the fluorescein-terminated polymers contained one dye molecule. These polymer samples were loaded with three different types of lanthanide ions as well as palladium and platinum ions. The numbers of metal atoms per chain were determined by a combination of UV/vis and conventional ICP-MS measurements. The experiments with lanthanide ions demonstrated that a net anionic charge on the polymer is important for water solubility. These experiments also showed that at least one type of lanthanide ion (La(3+)) is capable of forming a bimetallic complex with pendant DTPA groups. Conditions were developed for loading these polymers with palladium and platinum ions. While these polymers could be conjugated to antibodies, the presence of Pd or Pt ions in the polymer interfered with the ability of the antibody to recognize its antigen. For example, a goat anti-mouse (secondary) antibody labeled with polymers that contain Pd or Pt no longer recognized a primary antibody in a sandwich assay. In mass cytometry assays, these Pd- or Pt-containing MCPs were very effective in recognizing dead cells and provide a new and robust assay for distinguishing live cells from dead cells.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%