Pleural Lubrication

Porta, Cristina; Sironi, Chiara; Bodega, Francesca; Agostoni, Emilio

doi:10.3390/lubricants4020015

Cited by 5 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean value of µ was 0.0175 ± 0.001. This value was similar to those measured on pleural tissue specimens (approximately 0.025) [19], and on mesothelial cells in vitro (0.021 ± 0.001 and 0.032 ± 0.002 in 4/4 RM-4 and CARM-L1 TG3 cell line, respectively) [13]. Chen et al [14] found that the surface sliding friction of endothelial cell monolayers cultured on soft PNaSS gel was much greater than that exerted on the walls of blood vessels in vivo.…”

Section: Discussionsupporting

confidence: 89%

Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

Bodega

Sironi

Zocchi

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

To study the friction of cell monolayers avoiding damage due to stress concentration, cells can be cultured on fibrin gels, which have a structure and viscoelasticity similar to that of the extracellular matrix. In the present research, we studied different gel compositions and surface coatings in order to identify the best conditions to measure friction in vitro. We examined the adhesion and growth behavior of mesothelial cell line MET-5A on fibrin gels with different fibrinogen concentrations (15, 20, and 25 mg/mL) and with different adhesion coatings (5 μg/mL fibronectin, 10 μg/mL fibronectin, or 10 μg/mL fibronectin + 10 μg/mL collagen). We also investigated whether different substrates influenced the coefficient of friction and the ability of cells to stick to the gel during sliding. Finally, we studied the degradation rates of gels with and without cells. All substrates tested provided a suitable environment for the adherence and proliferation of mesothelial cells, and friction measurements did not cause significant cell damage or detachment. However, in gels with a lower fibrinogen concentration, cell viability was higher and cell detachment after friction measurement was lower. Fibrinolysis was negligible in all the substrates tested.

show abstract

Section: Discussionsupporting

confidence: 89%

Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

Bodega

Sironi

Zocchi

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The potential for therapeutic manipulation of these diverse functions has been complicated by the presence of a carbohydrate surface layer—here, called the mesopolysaccharide (MPS)—that coats the outer layer of the mesothelium. Although the structure and function of the MPS is not fully-understood, a common understanding is that the MPS creates a frictionless surface facilitating the movement of the ventilating lung, beating heart and peristaltic bowel [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Mesopolysaccharides: The extracellular surface layer of visceral organs

et al. 2020

View full text Add to dashboard Cite

The mesothelium is a dynamic and specialized tissue layer that covers the somatic cavities (pleural, peritoneal, and pericardial) as well as the surface of the visceral organs such as the lung, heart, liver, bowel and tunica vaginalis testis. The potential therapeutic manipulation of visceral organs has been complicated by the carbohydrate surface layer-here, called the mesopolysaccharide (MPS)-that coats the outer layer of the mesothelium. The traditional understanding of MPS structure has relied upon fixation techniques known to degrade carbohydrates. The recent development of carbohydrate-preserving fixation for high resolution imaging techniques has provided an opportunity to reexamine the structure of both the MPS and the visceral mesothelium. In this report, we used high pressure freezing (HPF) as well as serial section transmission electron microscopy to redefine the structure of the MPS expressed on the murine lung, heart and liver surface. Tissue preserved by HPF and examined by transmission electron microscopy demonstrated a pleural MPS layer 13.01±1.1 um deep-a 100-fold increase in depth compared to previously reported data obtained with conventional fixation techniques. At the base of the MPS were microvilli 1.1±0.35 um long and 42±5 nm in diameter. Morphological evidence suggested that the MPS was anchored to the mesothelium by microvilli. In addition, membrane pits 97±17 nm in diameter were observed in the apical mesothelial membrane. The spatial proximity and surface density (29±4.5%) of the pits suggested an active process linked to the structural maintenance of the MPS. The striking magnitude and complex structure of the MPS indicates that it is an important consideration in studies of the visceral mesothelium.

show abstract

“…Movement promotes HA turnover, whereas immobilization favors accumulation [13]. Mesothelial cells secrete HA which play chemo-mechanical roles favoring lubrication and water homeostasis in pleural, pericardial, and peritoneal fluids, therefore contributing to vital physiological functions [14,15].…”

Section: Ecm In Physiological Tissue Biomechanics and Adaptationmentioning

confidence: 99%

Extracellular matrix biomechanical roles and adaptation in health and disease

Franchi,

Piperigkou,

Mastronikolis

et al. 2023

The FEBS Journal

View full text Add to dashboard Cite

Extracellular matrices (ECMs) are dynamic 3D macromolecular networks that exhibit structural characteristics and composition specific to different tissues, serving various biomechanical and regulatory functions. The interactions between ECM macromolecules, such as collagen, elastin, glycosaminoglycans (GAGs), proteoglycans (PGs), fibronectin and laminin, along with matrix effectors and water, contribute to the unique cellular and tissue functional properties during organ development, tissue homoeostasis, remodeling, disease development and progression. Cells adapt to environmental changes by adjusting the composition and array of ECM components. ECMs forming the 3D bioscaffolds of our body provide mechanical support for tissues and organs and respond to the environmental variables influencing growth and final adult body shape in mammals. Different cell types exhibit specific adaptations to their respective ECM environments. ECMs regulate biological processes by controlling diffusion of infections/inflammations, sensing and adapting to external stimuli and gravity from the surrounding habitat, and, in the context of cancer, interplaying with and regulating cancer cell invasion and drug resistance. Alterations in the ECM composition in pathological conditions drive adaptive responses of cells and could therefore result in abnormal cell behavior and tissue dysfunction. Understanding the biomechanical functionality, adaptation and roles of distinct ECMs is essential for research into various pathologies, including cancer progression and multidrug resistance, which is of crucial importance for developing targeted therapies. In this Viewpoint article, we critically present and discuss specific biomechanical functions of ECMs and regulatory adaptation mechanisms in both health and disease, with a particular focus on cancer progression.

show abstract

Pleural Lubrication

Cited by 5 publications

References 52 publications

Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells

Mesopolysaccharides: The extracellular surface layer of visceral organs

Extracellular matrix biomechanical roles and adaptation in health and disease

Contact Info

Product

Resources

About