Pleurotus opuntiae revisited – An insight to the phylogeny of dimitic Pleurotus species with emphasis on the P. djamor complex

Zervakis, Georgios I.; Venturella, Giuseppe; Fryssouli, Vassiliki; Inglese, Paolo; Polemis, Elias; Gargano, Maria Letizia

doi:10.1016/j.funbio.2018.12.005

Cited by 19 publications

(12 citation statements)

References 43 publications

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“…Indicative cases are illustrated by boxplot graphs depicting genetic distances and sequence similarities in the main clades, as well as within and amongst selected species of the genus Ganoderma. Widely-adopted thresholds for separating amongst species in Basidiomycota are < 97% to 98% for ITS sequence similarity and > 0.010 to 0.020 for genetic distance uncorrected p-values (Smith et al 2007;Hughes et al 2009;Matheny et al 2009;Schoch et al 2012;Kondo et al 2018;Vu et al 2018;Zervakis et al 2019). Although such values were generally taken into account in this study, they were not found suitable for universal application in the genus Ganoderma.…”

Section: G Multiplicatum (17)mentioning

confidence: 88%

A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa

Fryssouli¹,

Zervakis²,

Polemis³

et al. 2020

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Ganoderma P. Karst. is a cosmopolitan genus of white-rot fungi which comprises species with highly-prized pharmaceutical properties, valuable biotechnological applications and of significant phytopathological interest. However, the status of the taxonomy within the genus is still highly controversial and ambiguous despite the progress made through molecular approaches. A metadata analysis of 3908 nuclear ribosomal internal transcribed spacer (ITS) rDNA sequences obtained from GenBank/ENA/DDBJ and UNITE was performed by targeting sequences annotated as Ganoderma, but also sequences from environmental samples and from material examined for the first time. Ganoderma taxa segregated into five main lineages (Clades A to E). Clade A corresponds to the core of laccate species and includes G. shanxiense and three major well-supported clusters: Cluster A.1 (‘G. lucidum sensu lato’) consists of taxa from Eurasia and North America, Cluster A.2 of material with worldwide occurrence including G. resinaceum and Cluster A.3 is composed of species originating from all continents except Europe and comprises G. lingzhi. Clade B includes G. applanatum and allied species with a Holarctic distribution. Clade C comprises taxa from Asia and Africa only. Clade D consists of laccate taxa with tropical/subtropical occurrence, while clade E harbours the highest number of non-laccate species with a cosmopolitan distribution. The 92 Ganoderma-associated names, initially used for sequences labelling, correspond to at least 80 taxa. Amongst them, 21 constitute putatively new phylospecies after our application of criteria relevant to the robustness/support of the terminal clades, intra- and interspecific genetic divergence and available biogeographic data. Moreover, several other groups or individual sequences seem to represent distinct taxonomic entities and merit further investigation. A particularly large number of the public sequences was revealed to be insufficiently and/or incorrectly identified, for example, 87% and 78% of entries labelled as G. australe and G. lucidum, respectively. In general, ITS demonstrated high efficacy in resolving relationships amongst most of the Ganoderma taxa; however, it was not equally useful at elucidating species barriers across the entire genus and such cases are outlined. Furthermore, we draw conclusions on biogeography by evaluating species occurrence on a global scale in conjunction with phylogenetic structure/patterns. The sequence variability assessed in ITS spacers could be further exploited for diagnostic purposes.

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Section: G Multiplicatum (17)mentioning

confidence: 88%

A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa

Fryssouli¹,

Zervakis²,

Polemis³

et al. 2020

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“…Previous results have highlighted the importance of mushrooms of the genus Pleurotus in human nutrition and in the feeding of farm animals [31][32][33][34][35]. Particular attention is also drawn to edible mushroom species that are infrequent/rare or linked to particular habitats (e.g., Pleurotus nebrodensis and P. opuntiae) [36,37], and for which only few or no studies exist regarding their nutritional and medicinal properties.…”

Section: Ecological and Conservation Issuesmentioning

confidence: 99%

Ecology, Phylogeny, and Potential Nutritional and Medicinal Value of a Rare White “Maitake” Collected in a Mediterranean Forest

et al. 2020

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Albino Grifola frondosa (Dicks.) Gray “maitake” mushrooms (described as G. albicans Imazeki and then placed in synonymy with G. frondosa) are particularly rare, and the few pertinent records are not treated in scientific publications. A field investigation carried out in Sicily (Italy) led to the collection of an unusual white Grifola specimen at the base of a living tree of Quercus pubescens Willd. s.l. The outcome of sequencing the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) indicated that it belongs to G. frondosa and provided an insight to the phylogenetic relationships within the genus. The results of nutritional composition analysis showed that the albino basidioma possesses relatively high contents of Ca, Fe, K, and Cu and is rather low in Na when compared with literature data on edible mushrooms. Vitamin (B1, B2, B3, B5, B9, and D2) contents ranged from 0.15 to 3.89 mg per 100 g of mushroom dry weight. The cold-water extract of this specimen was effective at inhibiting the growth of Staphylococcus epidermidis ATCC 12228 and Pseudomonas aeruginosa ATCC 15442 at the maximum screening concentration of 50% v/v. In addition, the extract slowed down the ability of Staphylococcus aureus ATCC 43300 to form biofilms. According to data hereby reported, the albino G. frondosa is a culinary-medicinal mushroom with a promising exploitation potential.

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“…The mushrooms that grow on plants of the genera Opuntia, Yucca, Agave, and Phytolacca, among others, are described as P. opuntiae, but because worldwide the genus Pleurotus includes several taxa and species, controversy exists due to the absence of valid sequencing data and/or phylogenetic reconstructions. This has generated signi cant ambiguities regarding the exact identity and distribution of P. opuntiae (Zervakis et al 2019). The mushroom P. djamor has a cosmopolitan distribution that includes species identi ed as P. ostreatoroseus, P. parsonsiae, and P. salmoneostramineus.…”

Section: Discussionmentioning

confidence: 99%

“…The mushroom P. djamor has a cosmopolitan distribution that includes species identi ed as P. ostreatoroseus, P. parsonsiae, and P. salmoneostramineus. P. djamor is a pantropical species found in wild form in several countries in the America, including Mexico, though its distribution is probably much broader (Zervakis et al 2019). Molecular studies have of 600-800 pb have been reported for P. abalonus, P. cystidiosus, P. cystidiosus var.…”

Section: Discussionmentioning

confidence: 99%

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Biological efficiency and nutritional composition of Pleurotus djamor cultivated on bagasse of Agave salmiana

Lucio¹,

Domínguez²,

Jiménez³

et al. 2021

Preprint

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The objective of the present study was to use molecular techniques to identify a wild mushroom isolated from A. salmiana, and then evaluate its biological efficiency, production rate, and nutritional and morphological characteristics when grown on A. salmiana bagasse with various concentrations of urea as a source of nitrogen. Two types of inoculum were employed: in grain (WG) and pellet (WP) form. The substrate was supplemented with total nitrogen concentrations (TN) of 0.77, 0.95, 1.14, 1.32, and 1.5% to evaluate its effect on the biological efficiency (BE), production rate (PR), and morphological and nutritional characteristics of the fruiting bodies. The molecular analysis of the ITS region permitted the amplification of a product of 750 pb. The mushroom was identified as Pleurotus djamor. After supplementing the substrate with urea, a BE of 70% was obtained in the sample inoculated with WG at 1.32% TN. Observations found that the TN concentration of 1.5% produced malformations in the fruiting bodies. The analysis of the sporocarps indicated a raw protein content (RP) of 15–26% and that the mushroom’s nutritional composition changed according to the inoculum utilized and the percentage of nitrogen in the substrate. This is the first report on the isolation of P. djamor on A. salmiana as an atypical substrate, and so represents an opportunity for further study and commercialization. To its chemical composition and high availability, A. salmiana bagasse is a suitable alternative substrate for cultivating edible mushrooms, specifically P. djamor.

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Pleurotus opuntiae revisited – An insight to the phylogeny of dimitic Pleurotus species with emphasis on the P. djamor complex

Cited by 19 publications

References 43 publications

A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa

A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa

Ecology, Phylogeny, and Potential Nutritional and Medicinal Value of a Rare White “Maitake” Collected in a Mediterranean Forest

Biological efficiency and nutritional composition of Pleurotus djamor cultivated on bagasse of Agave salmiana

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