2007
DOI: 10.1523/jneurosci.1132-07.2007
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PlexinA3 Restricts Spinal Exit Points and Branching of Trunk Motor Nerves in Embryonic Zebrafish

Abstract: The pioneering primary motor axons in the zebrafish trunk are guided by multiple cues along their pathways. Plexins are receptor components for semaphorins that influence motor axon growth and path finding. We cloned plexinA3 in zebrafish and localized plexinA3 mRNA in primary motor neurons during axon outgrowth. Antisense morpholino knock-down led to substantial errors in motor axon growth. Errors comprised aberrant branching of primary motor nerves as well as additional exit points of axons from the spinal c… Show more

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Cited by 39 publications
(45 citation statements)
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“…Our results clearly demonstrate that intraspinal guidance requires Plexin A3 signaling, which propagates the repulsive activities of semaphorins. Our model is consistent with recent results obtained by Feldner et al using plexin A3 as well as semaphorin 3A morpholinos (Feldner et al, 2007), as well as by Tanaka et al (Tanaka et al, 2007). Although this does not exclude the co-existence of a chemoattractive mechanism, our results present compelling genetic evidence that a particular guidance mechanism -repulsion -guides the intraspinal migration of motor growth cones.…”
Section: Research Reportsupporting
confidence: 93%
“…Our results clearly demonstrate that intraspinal guidance requires Plexin A3 signaling, which propagates the repulsive activities of semaphorins. Our model is consistent with recent results obtained by Feldner et al using plexin A3 as well as semaphorin 3A morpholinos (Feldner et al, 2007), as well as by Tanaka et al (Tanaka et al, 2007). Although this does not exclude the co-existence of a chemoattractive mechanism, our results present compelling genetic evidence that a particular guidance mechanism -repulsion -guides the intraspinal migration of motor growth cones.…”
Section: Research Reportsupporting
confidence: 93%
“…Morpholino oligonucleotides (MOs) against sema3D, nrp1A, L1.1, L1.2, plexinA3, and plexinA4 were synthesized by Gene Tools (Corvallis, OR). Sequences for morpholinos targeting Sema3D (Sema3DMO or Sema3D splice MO), Nrp1A (Nrp1AMO), PlexinA3 (PlexinA3MO1), PlexinA4 (PlexinA4MO), and control morpholinos (CONMO, STDCON) have been reported previously and tested for efficacy Miyashita et al, 2004;Wolman et al, 2004;Liu and Halloran, 2005;Feldner et al, 2007). The sequences of morpholinos targeting L1.1 (L1.1MOA and L1.1MOB) and the L1.2 (L1.2MOA and L1.2MOB) morpholinos are as follows (with the sequence complementary to the start codon underlined or residue numbers in parentheses for sequences not overlapping with start codon):…”
Section: Methodsmentioning
confidence: 99%
“…However, two identified zebrafish class A Plexins, plexinA3 and plexinA4, are expressed in the nucMLF (Miyashita et al, 2004;Feldner et al, 2007). We injected morpholinos against PlexinA3, PlexinA4, or both at previously published full-strength doses (Miyashita et al, 2004;Feldner et al, 2007). However, neither knockdown of PlexinA3 or PlexinA4 individually nor combined knockdown of both caused significant MLF defasciculation (data not shown).…”
Section: Sema3d Regulation Of L11 Is Critical For Mlf Fasciculationmentioning
confidence: 95%
See 1 more Smart Citation
“…A recent key word search of PubMed revealed 8596 publications on the use of zebrafish in vertebrate development. Some of the examples include development of the cardiovascular system [29], the endoderm [30], motor neurons [31,32] and craniofacial structures [33]. The insights gained from zebrafish are directly applicable to humans since molecular mechanisms that regulate vertebrate development are highly conserved between the two species.…”
Section: Modeling Vertebrate Developmentmentioning
confidence: 99%