Plicosepalus acacia Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling

Abdel-Hamed, Asmaa R.; Mehanna, Eman T.; Hazem, Reem M.; Badr, Jihan M.; Abo-Elmatty, Dina M.; Abdel‐Kader, Maged S.; Goda, Marwa S.

doi:10.3390/antiox10111701

Cited by 23 publications

(19 citation statements)

References 128 publications

(147 reference statements)

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“…This metabolomic analysis manifested the presence of coumarins, flavonoids, flavonoid glycosides, phenolic acids, sterols, terpenes, terpenoid bitter principles, and alkaloids. These metabolites were deduced depending on their mass accuracy, which is expressed in parts per million (ppm) error [ 37 , 43 , 47 ], as well as matching their MS/MS ion transitions with those reported in literature ( Table 3 , Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

“…High-performance liquid chromatography combined with the triple time-of-flight tandem mass spectrometry (LC/Triple-TOF-MS/MS) method was conducted as previously mentioned in detail [ 37 , 38 , 39 ]. An amount of 75 mg of the ethanolic crude extract of A. judaica L. were dissolved in 1.5 mL of a mixture containing water: methanol: acetonitrile (50:25:25) that was then ultra-sonicated and then centrifuged at 10,000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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In Vitro and In Vivo Studies of Anti-Lung Cancer Activity of Artemesia judaica L. Crude Extract Combined with LC-MS/MS Metabolic Profiling, Docking Simulation and HPLC-DAD Quantification

et al. 2021

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Artemisia judaica L. (Family: Asteraceae) exhibited antioxidant, anti-inflammatory, and antiapoptotic effects. The in vitro cytotoxic activity of A. judaica ethanolic extract was screened against a panel of cancer cell lines. The results revealed its cytotoxic activity against a lung cancer (A549) cell line with a promising IC50 of 14.2 μg/mL compared to doxorubicin as a standard. This was confirmed through the downregulation of antiapoptotic genes, the upregulation of proapoptotic genes, and the cell cycle arrest at the G2/M phase. Further in vivo study showed that a solid tumor mass was significantly reduced, with a tumor inhibition ratio of 54% relative to doxorubicin therapy in a Xenograft model. From a chemical point of view, various classes of natural products have been identified by liquid chromatography combined with tandem mass spectrometry (LC-MS/MS). The docking study of the detected metabolites approved their cytotoxic activity through their virtual binding affinity towards the cyclin-dependent kinase 2 (CDK-2) and epidermal growth factor receptor (EGFR) active sites. Finally, A. judaica is a fruitful source of polyphenols that are well-known for their antioxidant and cytotoxic activities. As such, the previously reported polyphenols with anti-lung cancer activity were quantified by high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD). Rutin, quercetin, kaempferol, and apigenin were detected at concentrations of 6 mg/gm, 0.4 mg/gm, 0.36 mg/gm, and 3.9 mg/gm of plant dry extract, respectively. It is worth noting that kaempferol and rutin are reported for the first time. Herein, A. judaica L. may serve as an adjuvant therapy or a promising source of leading structures in drug discovery for lung cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Studies of Anti-Lung Cancer Activity of Artemesia judaica L. Crude Extract Combined with LC-MS/MS Metabolic Profiling, Docking Simulation and HPLC-DAD Quantification

et al. 2021

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“…In March 2010, P. acacia and P. curviflorus were previously collected from the Saudi Arabian cities of Al-ula (in the Madina region) and Abha (in the Asir region), respectively [ 34 , 38 ]. The plants were authenticated in the Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia by Dr. Nahed Morad.…”

Section: Methodsmentioning

confidence: 99%

“…Chemical investigations of P. curviflorus resulted in the isolation and identification of triterpenes, phytosterols and polyphenolics: predominantly flavonols and flavan-3-ols [35][36][37][38][39][40]. Similarly, phenolic acids, flavonoids were reported in P. acacia in addition to loranthin a flavanocoumarin [26,34]. Interestingly, despite P. acacia and P. curviflorus are good sources of phenolic compounds, there are few scientific reports on the anticancer activity of these plants [36].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the antimicrobial effect of P. curviflorus and its cytotoxicity on FL-cells (a human amniotic epithelial cell line) have been reported [ 28 ]. On the other hand, P. acacia was verified to have antimicrobial potential [ 29 ], multi-organ protective effects [ 30 , 31 , 32 ], anti-diabetic activity [ 33 ] and an angiogenic effect against diabetes-induced hind ischemia [ 34 ]. The exhibited bioactivities of P. curviflorus and P. acacia are attributed to their phytoconstituents.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Assessment of the Antioxidant and Anticancer Activities of Plicosepalus acacia and Plicosepalus curviflorus: Metabolomic Profiling and In Silico Studies

et al. 2022

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This study presents a comparison between two mistletoe plants—P. acacia and P. curviflorus—regarding their total phenolic contents and antioxidant and anticancer activities. P. curviflorus exhibited a higher total phenolics content (340.62 ± 19.46 mg GAE/g extract), and demonstrated higher DPPH free radical scavenging activity (IC50 = 48.28 ± 3.41µg/mL), stronger reducing power (1.43 ± 0.54 mMol Fe+2/g) for ferric ions, and a greater total antioxidant capacity (41.89 ± 3.15 mg GAE/g) compared to P. acacia. The cytotoxic effects of P. acacia and P. curviflorus methanol extracts were examined on lung (A549), prostate (PC-3), ovarian (A2780) and breast (MDA-MB-231) cancer cells. The highest anticancer potential for the two extracts was observed on PC-3 prostate cancer cells, where P. curviflorus exhibited more pronounced antiproliferative activity (IC50 = 25.83 μg/mL) than P. acacia (IC50 = 34.12 μg/mL). In addition, both of the tested extracts arrested the cell cycle at the Pre-G1 and G1 phases, and induced apoptosis. However, P. curviflorus extract possessed the highest apoptotic effect, mediated by the upregulation of p53, Bax, and caspase-3, 8 and 9, and the downregulation of Bcl-2 expression. In the pursuit to link the chemical diversity of P. curviflorus with the exhibited bioactivities, its metabolomic profiling was achieved by the LC-ESI-TOF-MS/MS technique. This permitted the tentative identification of several phenolics—chiefly flavonoid derivatives, beside some triterpenes and sterols—in the P. curviflorus extract. Furthermore, all of the metabolites in P. curviflorus and P. acacia were inspected for their binding modes towards both CDK-2 and EGFR proteins using molecular docking studies in an attempt to understand the superiority of P. curviflorus over P. acacia regarding their antiproliferative effect on PC-3 cancer cells. Docking studies supported our experimental results; with all of this taken together, P. curviflorus could be regarded as a potential prospect for the development of chemotherapeutics for prostate cancer.

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Phenolic Profile, Bioactivities and In Silico Analysis of the Trunk Bark of Acacia Cyanophylla Lindl.

Beyaoui,

Kaplan,

Saidi

et al. 2024

Chemistry & Biodiversity

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In the current investigation, total phenolics and flavonoids of the methanolic extract obtained from the trunk bark of Acacia cyanophylla Lindl. were quantified by LC–HRMS technique. DPPH and ABTS reagents were employed to assay the antioxidant potential. The anti‐tyrosinase and anti‐α‐amylase potentials were also assayed. The findings revealed that thirteen polyphenolic compounds were detected in the methanolic extract with trans‐taxifolin (23.2 g/kg), as the major constituent. A. cyanophylla extract displayed a higher activity with DPPH test (IC50=10.14±1.00 μg/mL) than with ABTS (IC50=15.27±2.09 μg/mL). The same extract also exhibited interesting α‐amylase inhibitory action (IC50 value of 4.00±0.17 μg/mL). Moreover, methanolic trunk bark extract exerted strong anti‐tyrosinase capacity with an IC50 of 5.12±0.41 μg/mL in comparison to kojic acid (IC50=10.22±0.85 μg/mL) used as positive control. The antioxidant, anti‐tyrosinase and anti‐α‐amylase potentials of the methanolic extract of A. cyanophylla trunk bark were reinforced by in silico molecular docking analyses, which confirmed the results of the in vitro tests.

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Plicosepalus acacia Extract and Its Major Constituents, Methyl Gallate and Quercetin, Potentiate Therapeutic Angiogenesis in Diabetic Hind Limb Ischemia: HPTLC Quantification and LC-MS/MS Metabolic Profiling

Cited by 23 publications

References 128 publications

In Vitro and In Vivo Studies of Anti-Lung Cancer Activity of Artemesia judaica L. Crude Extract Combined with LC-MS/MS Metabolic Profiling, Docking Simulation and HPLC-DAD Quantification

In Vitro and In Vivo Studies of Anti-Lung Cancer Activity of Artemesia judaica L. Crude Extract Combined with LC-MS/MS Metabolic Profiling, Docking Simulation and HPLC-DAD Quantification

Comparative Assessment of the Antioxidant and Anticancer Activities of Plicosepalus acacia and Plicosepalus curviflorus: Metabolomic Profiling and In Silico Studies

Phenolic Profile, Bioactivities and In Silico Analysis of the Trunk Bark of Acacia Cyanophylla Lindl.

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