Ploidy Level Determinations in Buffalograss Clones and Populations

Johnson, Paul G.; Riordan, Terrance P.; Arumuganathan, K.

doi:10.2135/cropsci1998.0011183x003800020034x

Cited by 35 publications

(47 citation statements)

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“…Correlation between genome size and ploidy level is common in plants (Palomino et al, 2003) and similar highly significant correlations were also found in buffalograss (Bouteloua dactyloides) (Johnson et al, 1998) and fine fescues (Festuca spp.) (Huff and Palazzo, 1998).…”

Section: Discussionsupporting

confidence: 68%

Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

Mondal¹,

De²

2017

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In this study, 2C deoxyribonucleic acid (DNA) content and the genome sizes (in pictograms [pg] and megabase pairs [Mbp], respectively) of 19 promising commercial varieties of sugarcane, the derivatives of man-made interspecific hybrids between cultivated and wild species were analyzed using flow cytometry. In this work, 2C nuclear DNA content was determined. Knowing the 2C nuclear DNA content, the unknown chromosome numbers of the varieties could be predicted. Large differences (65% variation) in DNA content (2C) of 19 varieties were detected, ranging, from 3.80 to 10.96 pg, which corresponds to a genome size ranging from 3724.00 to 10740.80 Mbp due to the variation of ploidy level and are considered the most complex genomes among crop plants. However, the relationship between chromosome number and genome size was highly significant (P < 0.001). In this study, internode diameter, sugar juice content and cane yield/ha are also positively correlated with DNA content. The estimated genome sizes would also yield information critical for sugarcane breeding and genome sequencing programs.

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Section: Discussionsupporting

confidence: 68%

Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

Mondal¹,

De²

2017

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“…A flow cytometry investigation had previously been done by Johnson et al (1998) on approximately half of the genotypes used. Ploidy levels of the remaining genotypes were identified in this study (Table 1).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The linear correlation between the DNA contents of ploidy levels and their chromosome numbers indicates the ploidy series in buffalograss is probably due to autoploidy and multiplication of one genome. While diploid and tetraploid genotypes are mainly adapted to the southern portions of the Great Plains, hexaploid genotypes are found throughout the Great Plains region (Johnson et al 1998). Previous research (Huff et al 1993) has indicated diploid, tetraploid, and hexaploid types of buffalograss in the North American Great Plains, but in many cases they were indistinguishable, especially between tetraploids and hexaploids.…”

Section: Introductionmentioning

confidence: 98%

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Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers

et al. 2003

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Buffalograss [ Buchloe dactyloides (Nutt.) Englem] germplasm has a broad resource of genetic diversity that can be used for turfgrass, forage and conservation. Buffalograss is the only native grass that is presently used as a turfgrass in the Great Plains region of North America. Its low growth habit, drought tolerance and reduced requirement for fertilizer and pesticides contribute to interest in its use. The objectives of this study were to use sequence-related amplified polymorphism (SRAP) markers in the evaluation of genetic diversity and phenetic relationships in a diverse collection of 53 buffalograss germplasms, and to identify buffalograss ploidy levels using flow cytometry. Based on their DNA contents, buffalograss genotypes were grouped into four sets, corresponding to their ploidy levels. Thirty-four SRAP primer combinations were used. This is the first report of the detection of differentiating diploid, tetraploid, pentaploid and hexaploid buffalograss genotypes, representing diverse locations of origin, using SRAP markers. Cluster analysis by the unweighted pair-group method with arithmetic averages based on genetic similarity matrices indicated that there were eight clusters. The coefficients of genetic distance among the genotypes ranged from 0.33 up to 0.99 and averaged D=0.66. The genetic diversity estimate, He, averaged 0.35. These results demonstrated that genotypes with potential traits for turfgrass improvement could readily be distinguished, based on SRAP. The use of PCR-based technologies such as SRAP is an effective tool for estimating genetic diversity, identifying unique genotypes as new sources of alleles for enhancing turf characteristics, and for analyzing the evolutionary and historical development of cultivars at the genomic level in a buffalograss breeding program.

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“…Although a few dioecious plants have identifiable sex chromosomes (Wilby and Parker, 1986;Vyskot and Hobza, 2004), there is no reported evidence of sex chromosomes in buffalo grass. This may be attributed in part to the fact that karyotype analysis of buffalo grass is complicated by its ability to exist in diploid (2n = 20), tetraploid, or hexaploid states (Johnson et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Identification of a locus characteristic of male individuals of buffalo grass [Buchloe dactyloides (Nutt.) Engelm.] by using an RAPD marker

Wang²,

Yang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Buffalo grass [Buchloe dactyloides (Nutt.) Engelm.] plants can be either male, female, or hermaphrodite (monoecious). As there is no morphological difference in the early vegetative growth of these three classes of plants, it is worthwhile to use molecular biological methods to attempt to identify the sex of a plant at this early growth period. In this study, we identified 23 plants that had a stable sex for over at least 3 years. Of these, 9 were male plants, 10 were female plants, and 4 were hermaphrodites. Screening of 300 RAPD primers identified a primer, namely S211 (5'-ttccccgcga-3'), which is capable of identifying male plants. The specific fragment was cloned, sequenced, and submitted to the GenBank database Identification of buffalo grass male individuals using RAPD (accession No. JN982469). When used to identify the sex of 188 plants during their first growing season, the S211 primer correctly identified 85.8% of all male plants. Our results showed that the S211 primer can identify the male, and in doing so, it facilitates buffalo grass breeding work.

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Ploidy Level Determinations in Buffalograss Clones and Populations

Cited by 35 publications

References 0 publications

Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers

Identification of a locus characteristic of male individuals of buffalo grass [Buchloe dactyloides (Nutt.) Engelm.] by using an RAPD marker

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