Plum Cultivar Dna Polymorphism Generated With Rapd and Issr Markers

Lisek, Anna; Korbin, M.; Rozpara, E.; Žurawicz, E.

doi:10.17660/actahortic.2007.734.36

Cited by 8 publications

(12 citation statements)

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“…In fact, we obtained a high number polymorphic bands (147), which is considerably important compared to other results reported by Shimada et al (1999) and Lisek et al (2007) using 20 and 13 RAPD primers, respectively. Polymorphisms at the DNA level have been used in many investigations to determine the genetic diversity in plums.…”

Section: Discussionsupporting

confidence: 43%

“…Polymorphisms at the DNA level have been used in many investigations to determine the genetic diversity in plums. Previous molecular studies on plums using RAPD markers revealed a wide genetic polymorphism among different plum accessions ranging from 20 to 98% (Gregor et al, 1994;Ortiz et al, 1997;Bellini et al, 1998;Shimada et al, 1999;Lisek et al, 2007;Liu et al, 2007;Hend et al, 2009). This wide variation is normal because plums are very complex groups including diploid, tetraploid and hexaploid species and because flower biology also differs in different plum groups (Ercisli, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Molecular marker techniques may offer great promise to determine genetic diversity in plant species. Polymerase chain reaction (PCR)-based markers have been extensively applied in genetic diversity studies in plums (Gregor et al, 1994;Shimada et al, 1999;Goulao et al, 2001;Lisek et al, 2007;Ayanoglu et al, 2007;Hend et al, 2009;Ilgin et al, 2009). All these studies revealed reliable genetic differences among materials.…”

Section: Introductionmentioning

confidence: 99%

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An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Ertürk¹,

Erċışlı²,

Maghradze³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.

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Section: Discussionsupporting

confidence: 43%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Ertürk¹,

Erċışlı²,

Maghradze³

et al. 2009

Genet. Mol. Res.

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“…on the other hand, the accessions were most tightly clustered by their species. Previous studies on the relationships of the plum accessions conducted by RAPD and ISSR confirm the suitability of molecular markers for the diversification of plum cultivars (4,11).…”

Section: Resultsmentioning

confidence: 56%

Molecular Characterization of Plum Cultivars by AFLP Markers

Ilgin

Kafkas

Erċışlı

2009

Biotechnology & Biotechnological Equipment

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“…Many studies have been carried on the plum cultivars, and important results have been obtained using RAPD markers (Shimada et al, 1999;Casas et al, 1999;Liu et al, 2007 andLisek et al, 2007). Hend et al (2009) studied 27 plum cultivars with a combination of morphological, pomological and RAPD markers and found considerable ge-netic diversity among them.…”

Section: Introductionmentioning

confidence: 99%

Molecular and morphological discrimination of selected plum seedlings for rootstock breeding

Aran

Fatahi

Zamani

2012

Journal of Fruit and Ornamental Plant Research

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A B S T R A C TPlum seedlings are extensively used as rootstocks for stone fruits except P. cerasus. Genetic diversity in seedlings used for rootstocks might result in variation of scion performance and subsequently non-uniform orchards. This study aimed on investigating variation among several Iranian plum genotypes sampled from seedlings grown for grafting. Thirty-five 3-to 4-year-old seedlings were characterized using 28 quantitative and qualitative traits, as well as 22 RAPD markers. Results of the morphological study revealed remarkable variability in studied traits. Genotypes were separated into three different groups according to their height using cluster analysis performed by Ward's clustering method based on morphological data. Twenty two RAPD primers from 120 screened produced 195 polymorphic reproducible bands (86.75% polymorphism). According to the similarity matrix, the lowest similarity was obtained between the genotype Tansgol (control) and S4-13 and the highest similarity between S11-25 and S11-26. According to the cluster analysis based on Jaccard's similarity coefficients and the UPGMA method at a similarity level of 0.56, the genotypes were divided into six sub-clusters. Significant association between eight morphological traits with RAPD markers was found by marker association analysis done using multiple linear regressions. The application of the methodology in screening elite genotypes is discussed.

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Plum Cultivar Dna Polymorphism Generated With Rapd and Issr Markers

Cited by 8 publications

References 0 publications

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Molecular Characterization of Plum Cultivars by AFLP Markers

Molecular and morphological discrimination of selected plum seedlings for rootstock breeding

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