Plumbagin reduces chronic lymphocytic leukemia cell survival by downregulation of Bcl-2 but upregulation of the Bax protein level

Fu, Chunling; Gong, Yanqing; Shi, Xuanxuan; Sun, Zengtian; Niu, Mingshan; Sang, Wei; Xu, Linyan; Feng, Zheng; Wang, Ying; Xu, Kailin

doi:10.3892/or.2016.4950

Cited by 15 publications

(6 citation statements)

References 24 publications

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“…Its downregulation following the application of miR-204-5p inhibitors was identified in BRO cells, and following the application of miR-204-5p mimics in SK-MEL1 cells. As apoptosis was not triggered by miRNA modulation, the observed Bcl-2 dysregulation may be implicated in non-apoptotic functions, including cell survival, in melanoma cells ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

miR‑204‑5p and miR‑3065‑5p exert antitumor effects on melanoma cells

et al. 2018

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MicroRNA (miR)-204-5p was previously identified to be downregulated in melanoma compared with melanocytic nevi. This observation prompted a functional study on miR-204-5p and the newly-identified miR-3065-5p, two miRNAs suggested to be tumor-suppressive oncomiRs. Application of miR-204-5p mimics or inhibitors resulted in a decrease or increase, respectively, in melanoma cell proliferation and colony formation. miR-204-5p mimics hindered invasion, whereas miR-204-5p inhibitors stimulated cancer cell migration. Modulation of miR-3065-5p led to a decrease in melanoma cell proliferation, altered cell cycle distribution and increased expression levels of its target genes HIPK1 and ITGA1, possibly due to functional modifications identified in these cells. miR-204-5p and miR-3065-5p demonstrated antitumor capacities that may need to be taken into account in the development of melanoma treatment approaches.

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Section: Discussionmentioning

confidence: 99%

miR‑204‑5p and miR‑3065‑5p exert antitumor effects on melanoma cells

et al. 2018

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“…A range of pharmacological activities including antimicrobial, hypolipidemic, and antitumor effects, have been ascribed to this compound (14)(15)(16). The antitumor activities have been demonstrated in a wide range of cancer types and cell lines, in in vitro and in vivo studies (17)(18)(19)(20)(21). However, despite the growing body of studies on the antitumor effect of plumbagin, there are no reports on the cytotoxic responses of metastatic retinoblastoma to this compound.…”

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confidence: 99%

Plumbagin Induces CytotoxicityviaLoss of Mitochondrial Membrane Potential and Caspase Activation in Metastatic Retinoblastoma

et al. 2021

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Background/Aim: We investigated the cytotoxic effects of plumbagin on metastatic retinoblastoma, using the highly metastatic cell line Y79. Materials and Methods: Effect of plumbagin on cell growth was assessed with water-soluble tetrazolium 1 (WST-1) cell proliferation assay and automated hemocytometry with trypan blue-exclusion assay. Cell death was studied with acridine orange/ethidium bromide live-dead assay and annexin-V-fluorescein isothiocyanate/propidium iodide microscopy. Loss of mitochondrial membrane potential was studied with JC-10 dye and caspase activation was investigated using CellEvent Caspase-3/7 Green detection reagent. Results: Plumbagin highly significantly reduced the growth of Y79 cells treated for 24 h with 2.5 μM or more. Plumbagin also induced significantly high levels of cell death which was associated with loss of mitochondrial membrane potential and caspase activation. Conclusion: At very low concentration (2.5 μM), plumbagin potently induced cytotoxicity in metastatic retinoblastoma cells via loss of mitochondrial membrane potential and caspase activation.

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“…Both Bcl‐2 and Bax can exist in the form of homodimers, and they can also form heterodimers. Cells with a small amount of Bax require only low levels of Bcl‐2 to form the Bcl‐2‐Bax complex, which is strictly stoichiometrically, thereby stopping the occurrence of apoptosis . The expression of Bcl‐2 protein can be found to be significantly reduced in various complex refractory skin ulcers, while the expression of Bax protein is abnormally enhanced, resulting in increased cell apoptosis, delayed wound healing, etc.…”

Section: Discussionmentioning

confidence: 99%

Effect of hyperbaric oxygen on the process of hypertrophic scar formation in rabbit ears

Ren

Liu

Wan

et al. 2018

J of Cosmetic Dermatology

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SummaryObjective: To explore the influence of hyperbaric oxygen on scar formation in rabbit ears.Methods: A total of 20 New Zealand rabbits were selected to establish the hypertrophic scar model on the ears. The rabbits were randomly divided into control group and experimental group (7d, 14d, 21d, and 28d group according to different HBO treatment days),each experimental group received hyperbaric oxygen treatment after the operation at the same time everyday for 1 hour. After the day 29, the scars were collected. Histomorphological change in scars was observed by hematoxylin-eosin staining, Masson staining, and transmission electrical microscope. The expression of bax, bcl-2, and the cell apoptosis rate was detected by immunohistochemical method.Results: (i) Both number of fibroblast and amount of collagen fibrils in experimental group were significantly reduced compared with those in control group. In Masson staining, arrangement of collagen fibrils in experimental group was much more irregular and coarse than control groups. (ii) HI value can be found much smaller in the experimental groups than the control (P < .05). Among the four experimental groups, there is significant difference among 7d, 14d, and 21d groups (P < .05), while there is no difference between 21d and 28d groups (P > .05). (iii) Expression of Bax could be detected up-regulated in experimental group (P < .05). While the expression of Bcl-2 is detected significantly down-regulated in experimental group than that in control group (P < .05). Compared with the 7d group, the expression of Bax and Bcl-2 has significant difference in 14d group (P < .05), and the expression of this two factors in 21d group has significant difference comparing with 14d group(P < .05),but there is no significant difference between 28d group and 21d group(P > .05). (iv) Significant difference of cell apoptosis rate can be detected between the experimental groups and the control group (P < .05). Among the four experimental groups, there is significant difference among 7d, 14d, and 21d groups (P < .05), while there is no difference between 21d and 28d groups (P > .05). Conclusion:The hyperbaric oxygen can up-regulate bax/bcl-2 value, increase the cell apoptosis rate, and inhibit the early hypertrophic scar in rabbit ears.bax, bcl-2, cell apoptosis rate, hyperbaric oxygenationThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Plumbagin reduces chronic lymphocytic leukemia cell survival by downregulation of Bcl-2 but upregulation of the Bax protein level

Cited by 15 publications

References 24 publications

miR‑204‑5p and miR‑3065‑5p exert antitumor effects on melanoma cells

miR‑204‑5p and miR‑3065‑5p exert antitumor effects on melanoma cells

Plumbagin Induces CytotoxicityviaLoss of Mitochondrial Membrane Potential and Caspase Activation in Metastatic Retinoblastoma

Effect of hyperbaric oxygen on the process of hypertrophic scar formation in rabbit ears

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