Pluronic F-68: an answer for shoot regeneration recalcitrance in microspore-derived Brassica napus embryos

Abstract

Recalcitrance to tissue culture is observed in some genotypes of Brassica napus. Several studies have confirmed that Pluronic F-68 has growth-promoting effects on numerous tissue types. This work investigated the effect of the non-ionic surfactant Pluronic F-68 at four concentrations (0.1%, 0.25%, 0.5%, and 1% (w/v)) on the responsiveness of recalcitrant B. napus lines to tissue culture. Microspores from seven populations of B. napus were cultured on Nitsch and Nitsch medium with this compound. The embryos obtained were plated on solid B5 medium supplemented with zeatin for shoot induction. Pluronic F-68 had a highly significant effect on the proportion of shoot regeneration (P<0.05) in some of the recalcitrant populations. However, no strong dose-response effect was observed. The estimated probability of a shoot occurring in the absence of Pluronic F-68 ranged from 0.04 to 0.31 depending on the genotype, while in the presence of Pluronic F-68, it ranged from 0.07 to 0.53, respectively.

Pluronic F-68 Improves Callus Proliferation of Recalcitrant Rice Cultivar via Enhanced Carbon and Nitrogen Metabolism and Nutrients Uptake

“…Aside from protein abundance in plants, esterase could be used to determine cell viability. Several studies have utilized the changes in esterase activity as a marker to study the growth characteristics and cell viability in plants (Amano et al, 2003;Tamás et al, 2005;Víteček et al, 2007). In a way, a decrease in esterase activity in plants corresponds with the decrease in cell viability in plants.…”

“…Some of the commonly used growth additives in plant tissue culture include Pluronic F-68 (PF-68), lignosulfonate, silver nitrate, silicon, coconut, and gibberellic acid (Biswas and Mandal, 2007 ; He et al, 2013 ; Yildirim and Turker, 2014 ; Irshad et al, 2018 ; Wan Abdullah et al, 2020 ). PF-68 is a non-ionic, copolymer surfactant that has been employed as an additive in both in vitro animal and plant cultures (Meier et al, 1999 ; Barbulescu et al, 2011 ). PF-68 has been widely utilized in animal cell suspension culture to protect and repair damaged cells from constant sparging and agitation (Meier et al, 1999 ).…”

“…Besides, the application of PF-68 was reported to enhance shoot regeneration in Citrus sinensis (Curtis and Mirkov, 2012 ), Pyrus communis (Dashti et al, 2012 ), Ricinus communis (Kulathuran and Narayanasamy, 2015 ), and Abelmoschus esculentus (Irshad et al, 2018 ). Notably, PF-68 was demonstrated to improve the growth of roots, callus, and protoplast of Solanum dulcamara (Kumar et al, 1992 ), shoot regeneration of recalcitrant Brassica napus embryos (Barbulescu et al, 2011 ), and callus proliferation of recalcitrant indica rice (Kok et al, 2020 ). This implies that PF-68 could be a good candidate for plant cell growth and regeneration improvement of the recalcitrant cultivars.…”

Priming of Pisum sativum seeds with stabilized Pluronic P85 nanomicelles: effects on seedling development and photosynthetic function

“…Their interaction with human cells is also well studied (reviewed in Jarak et al 2020), however, little is known about plant cells. Nevertheless, there are reports demonstrating that at specific experimental conditions, Pluronic F-68 has growth-stimulating effects on protoplasts (Kumar et al 1992, Anthony et al 1997, plant cell/ tissue cultures (Kumar et al 1992, Iordan-Costache et al 1995, Khehra et al 1995, Anthony et al 1996, Cancino et al 2001, Lee and Kim 2002, Kaparakis and Alderson 2003, Khatun et al 2003, Kok et al 2021, and microspore cultures (Barbulescu et al 2011). The effect of Pluronics on intact plants of large crabgrass was studied by Nalewaja et al (1998) who showed that Pluronic P85 was one of the most effective adjuvants tested (among five types of Pluronic polymers) for the reduction of nicosulfuron herbicide phytotoxicity.…”

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

“…De-Xian Kok et al [34] demonstrated in their study that PF-68 has growth-stimulating effects without being dependent on the presence of plant hormones. The optimal concentration of PF-68 has improved the absorption of nutrients in plant tissue demonstrating the many beneficial effects of tissue regeneration and proliferation in recalcitrant species in vitro [34][35][36][37] as well as in P. edulis, P. giberti and P. mollissima [32].…”