Pneumatic mold-aided construction of a three-dimensional hydrogel microvascular network in an integrated microfluidics and assay of cancer cell adhesion onto the endothelium

Wang, Jian-Chun; Tu, Qin; Wang, Yaolei; Liu, Wenming; Li, Rui; Shen, Shaofei; Xu, Juan; Zhao, Lei; Wang, Jinyi

doi:10.1007/s10404-013-1172-2

Cited by 12 publications

(5 citation statements)

References 46 publications

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“…Two plates engraved with mirror image semi-circular channels can then be aligned and bonded to achieve a fully circular cross section that accurately reproduces in vivo cell partitioning effects [ 77 ]. With very soft materials such as hydrogels, a pneumatically actuated mold can be used to imprint a semi-circular vessel network during polymerization [ 78 ]. Although this technique does not permit fully circular cross section, it provides a more realistic matrix for endothelialization, which is increasingly employed in microfl uidic systems [ 45 , 46 , 78 , 79 ].…”

Section: Development Of Fabrication Techniques For Realistic Biomimetmentioning

confidence: 99%

Microfluidic Platforms for the Interrogation of Intravascular Cellular Trafficking Mechanisms Influenced by Hemodynamic Forces

McClatchey

Hannen

Thomas

2016

Microscale Technologies for Cell Engineering

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Section: Development Of Fabrication Techniques For Realistic Biomimetmentioning

confidence: 99%

Microfluidic Platforms for the Interrogation of Intravascular Cellular Trafficking Mechanisms Influenced by Hemodynamic Forces

McClatchey

Hannen

Thomas

2016

Microscale Technologies for Cell Engineering

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“…They found that the distance between the cancer cell and the wall played an important role in the cell adhesion, which was strongly dependent on the channel geometry. Wang et al 152 developed a microdevice with complex geometry, in which endothelium cells were cultured. They investigated the adhesive interactions between breast cancer cells and endothelial cells.…”

Section: Microfluidic Devices For Cell Adhesionmentioning

confidence: 99%

Hemodynamics in the Microcirculation and in Microfluidics

et al. 2014

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Hemodynamics in microcirculation is important for hemorheology and several types of circulatory disease. Although hemodynamics research has a long history, the field continues to expand due to recent advancements in numerical and experimental techniques at the micro-and nano-scales. In this paper, we review recent computational and experimental studies of blood flow in microcirculation and microfluidics. We first focus on the computational studies of red blood cell (RBC) dynamics, from the single cellular level to mesoscopic multiple cellular flows, followed by a review of recent computational adhesion models for white blood cells, platelets, and malaria-infected RBCs, in which the cell adhesion to the vascular wall is essential for cellular function. Recent developments in optical microscopy have enabled the observation of flowing blood cells in microfluidics. Experimental particle image velocimetry and particle tracking velocimetry techniques are described in this article. Advancements in micro total analysis system technologies have facilitated flowing cell separation with microfluidic devices, which can be used for biomedical applications, such as a diagnostic tool for breast cancer or large intestinal tumors. In this paper, cell-separation techniques are reviewed for microfluidic devices, emphasizing recent advances and the potential of this fast-evolving research field in the near future.

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“…Col I hydrogels can be obtained by neutralizing the pH of the acid collagen solution and incubating at 37 °C, a crosslinking mechanism that is highly compatible with cell encapsulation. Col I can be used as a standalone 3D hydrogel for tumor models establishment, or be combined with other biomaterials to generate multifunctional hydrogels with improved ECM‐mimetic properties that can be valuable for in vitro modeling of a number of cancers including breast cancer, [ 44,138–144 ] colorectal cancer, [ 145,146 ] glioblastoma multiform (GBM), [ 124,147,148 ] liver, [ 149–151 ] lung cancer, [ 111,152,153 ] osteosarcoma [ 154,155 ] or ovarian cancer, [ 156 ] among others. Col I hydrogel stiffness can be easily manipulated by tuning the hydrogel precursor solution concentration, covalent crosslinking density and the chemical moieties involved in this crosslink.…”

Section: Engineering the Tme Using Protein‐based Hydrogelsmentioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

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The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics.

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Pneumatic mold-aided construction of a three-dimensional hydrogel microvascular network in an integrated microfluidics and assay of cancer cell adhesion onto the endothelium

Cited by 12 publications

References 46 publications

Microfluidic Platforms for the Interrogation of Intravascular Cellular Trafficking Mechanisms Influenced by Hemodynamic Forces

Microfluidic Platforms for the Interrogation of Intravascular Cellular Trafficking Mechanisms Influenced by Hemodynamic Forces

Hemodynamics in the Microcirculation and in Microfluidics

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

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