Pneumopathie à Pneumocystis jirovecci chez une patiente immunocompétente

Nejmi, H.; Ziati, J.; Tijani, A.; Charaf, K.; Eladib, A.R.; Younous, S.; Samkaoui, M.A.; Zougari, L.

doi:10.1016/j.medmal.2009.08.006

Cited by 7 publications

(4 citation statements)

References 10 publications

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“…The diagnosis requires the detection of Pneumocystis carinii by appropriate coloring on respiratory samples (Gomori-Grocott, Giemsa, toluidine blue, specific immunofluorescence) [9], or molecular biology with the identification of Pneumocystis jiroveci DNA by polymerase chain reaction [6].…”

Section: Discussionmentioning

confidence: 99%

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<i>Pneumocystis jiroveci</i> Pneumonia in an Immunocompetent Female Patient

Àmro¹,

Hamzaoui²,

Sajiai³

et al. 2014

CRCM

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Introduction: The pneumocystosis is an infection caused by a saprophytic germ Pneumocystis jiroveci. It is exceptional in immunocompetent patients. Case report: This is a 30-year-old patient, admitted to the service for an acute respiratory failure. She was treated for pulmonary tuberculosis 6 months ago. The review has objectified clinique cyanosis of the lips and extremities with a blood pressure 120/70 mmHg, a heart rate of 70 bats/min, a temperature of 38˚C and SaO2 to 82% in the open air. The chest radiograph objectified reticulonodular opacities on the right. The patient was put under bi-broad-spectrum antibiotics. Due to the non improvement of the patient's state, the search for Pneumocystis jiroveci in induced sputum was made and it was positive. The search for a field of immunosuppression was negative.

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Section: Discussionmentioning

confidence: 99%

“…Corticosteroids have more interest in pneumocystoses unrelated to HIV. That said, the risk of an associated latent viral infection is important [6]. The prognosis of this affection remains conditioned by the severity of lung damage and early effective treatment.…”

Section: Discussionmentioning

confidence: 99%

<i>Pneumocystis jiroveci</i> Pneumonia in an Immunocompetent Female Patient

Àmro¹,

Hamzaoui²,

Sajiai³

et al. 2014

CRCM

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“…Pneumocystis jirovecii , a human-specific pathogenic fungus, causes life-threateningly severe Pneumocystis pneumonia (PCP) in immunocompromised patients, with 400,000 estimated cases per year worldwide [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…as stated in the article,2 was not used for design of our culture attempts,3 other media used: YEPD broth + agar, YM broth, wort broth + agar, BHI broth + agar, Sabouraud dextrose broth + agar, modified Sabouraud dextrose broth + agar, Vogel & Johnson agar, NPG medium.…”

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confidence: 99%

Axenic Long-Term Cultivation of Pneumocystis jirovecii

Riebold,

Mahnkopf,

Wicht

et al. 2023

JoF

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Pneumocystis jirovecii, a fungus causing severe Pneumocystis pneumonia (PCP) in humans, has long been described as non-culturable. Only isolated short-term experiments with P. jirovecii and a small number of experiments involving animal-derived Pneumocystis species have been published to date. However, P. jirovecii culture conditions may differ significantly from those of animal-derived Pneumocystis, as there are major genotypic and phenotypic differences between them. Establishing a well-performing P. jirovecii cultivation is crucial to understanding PCP and its pathophysiological processes. The aim of this study, therefore, was to develop an axenic culture for Pneumocystis jirovecii. To identify promising approaches for cultivation, a literature survey encompassing animal-derived Pneumocystis cultures was carried out. The variables identified, such as incubation time, pH value, vitamins, amino acids, and other components, were trialed and adjusted to find the optimum conditions for P. jirovecii culture. This allowed us to develop a medium that produced a 42.6-fold increase in P. jirovecii qPCR copy numbers after a 48-day culture. Growth was confirmed microscopically by the increasing number and size of actively growing Pneumocystis clusters in the final medium, DMEM-O3. P. jirovecii doubling time was 8.9 days (range 6.9 to 13.6 days). In conclusion, we successfully cultivated P. jirovecii under optimized cell-free conditions in a 70-day long-term culture for the first time. However, further optimization of the culture conditions for this slow grower is indispensable.

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