Point Analysis of Foods by Sheath-Flow Probe Electrospray Ionization/Mass Spectrometry (sfPESI/MS) Coupled with a Touch Sensor

Hiraoka, Kenzo; Rankin‐Turner, Stephanie; Ninomiya, Satoshi; Sekine, Ryo; Wada, Hiroshi; Matsumura, Masatoshi; Sanada‐Morimura, Sachiyo; Tanaka, Fukuyo; Nonami, Hiroshi; Ariyada, Osamu

doi:10.1021/acs.jafc.9b06489

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…Compared to the other AIMS approaches, sfPESI and zvPSI, which were used to analyze skin surface lipids, the lipid profile of TD-ESI/MS is unique. − Protonated SQ ions and cholesteryl ions (an in-source fragmentation product of cholesterol ester or the dehydrated free cholesterol ion), which belong to the nonpolar lipid category, were obviously detected by TD-ESI/MS, while they are absent in sfPESI and zvPSI systems. − The differences between the results obtained by these methods are likely due to their different ionization mechanisms. In general, analytes are ionized by proton or metal cation transfer in highly charged droplets in ESI, the low basicity of nonpolar lipids is disadvantaged in this kind of ionization.…”

Section: Resultsmentioning

confidence: 99%

“…The TD-ESI/MS system was set up in the same configuration as described in our previous publications. − The TD-ESI system is comprised of a direct sampling probe, thermal desorption unit, and electrospray ionization unit. The ions generated in the TD-ESI source were detected by a linear ion trap mass analyzer (LTQ XL, Thermo Fisher Scientific, Waltham, MA).…”

Section: Methodsmentioning

confidence: 99%

“…Some AIMS were used to analyze skin surface lipids. For instance, Hiraoka et al applied sheath-flow probe electrospray ionization (sfPESI) to sebum on the forehead and detected a number of lipids. , This method uses a fine plastic capillary filled with a solvent to extract samples on the surface, and electrospray mass spectra are obtained by applying a high voltage to the metal needle inserted in the capillary. Another AIMS, zero-volt paper spray ionization (zvPSI), was used to profile facial lipids collected as imprints onto a filter paper. , In this study, we use thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS) to rapidly characterize sebaceous lipids.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Mapping of Sebaceous Squalene by Ambient Mass Spectrometry

Cho¹,

et al. 2021

Anal. Chem.

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Squalene (SQ), a highly unsaturated sebaceous lipid, plays an important role in protecting human skin. To better understand the role of SQ in clinical medicine, an efficient analytical approach is needed to comprehensively study the distribution of SQ on different parts of the skin. In this study, sebaceous lipids were collected from different epidermal areas of a volunteer with sampling probes. Thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS) was then used to characterize the lipid species on the probes, and each TD-ESI/MS analysis was completed within a few seconds without any sample pretreatment. The molecular mapping of epidermal squalene on whole-body skin was rendered by scaling the peak area of the extracted ion current (EIC) of SQ based on a temperature color gradient, where colors were assigned to the 1357 sampling locations on a 3D map of the volunteer. The image showed a higher SQ distribution on the face than any other area of the body, indicating the role of SQ in protecting facial skin. The results were in agreement with previous studies using SQ as a marker to explore sebaceous activity. The novelty and significance of this work are concluded as two points: (1) direct and rapid detection of all major classes of sebaceous lipids, including the unsaturated hydrocarbons (SQ) and nonpolar lipids (e.g., cholesterol). The results are unique compared to other conventional and ambient ionization mass spectrometry methods and (2) this is the first study to analyze SQ distribution on the whole-body skin by a high-throughput approach.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Mapping of Sebaceous Squalene by Ambient Mass Spectrometry

Cho¹,

et al. 2021

Anal. Chem.

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“…In our previous study, TD-ESI/MS has been used to rapidly characterize sebaceous lipids [ 34 ]. Compared to the other AIMS approaches which were used to analyze skin surface lipids [ 30 – 33 ], the lipid profile of TD-ESI/MS is unique. All major classes of sebaceous lipids, including the unsaturated hydrocarbons (SQ) and nonpolar lipids (e.g.…”

Section: Introductionmentioning

confidence: 99%

The study of distribution of ingested terbinafine on skin with ambient ionization tandem mass spectrometry

Cho¹,

Su²,

Wu³

et al. 2022

Journal of Food and Drug Analysis

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We aim to develop an efficient and non-invasive strategy for monitoring of drugs and their metabolites via human skin. Probe sampling was combined with thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) to characterize trace terbinafine, which was secreted on patient’s skin after ingesting terbinafine tablets. The terbinafine ion signals were directly detected in the samples collected from different skin regions and the signals were monitored for 8 weeks. The detection and location of terbinafine via the skin suggest that the methods are useful in rapidly and noninvasively collecting the molecular information of the ingested drug on skin for pharmacokinetic studies.

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“…[23][24][25] Analytes are then extracted from a surface by touching the sfPESI probe to the sample surface, which can be either a wet or dried material, for 5 s in which liquid extraction from the surface occurs. 25 A high voltage (≤ 2.5 kV) is applied to facilitate an electrospray through the probe for a short duration, c. 5 s. [22][23][24][25][26] There are a number of advantages in this method. Due to the small diameter of the needle of the sfPESI probe, only about 1 mm 2 of the sample surface (Figure S1b [supporting information]) is affected by the extraction making it minimally destructive.…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid, in‐situ analysis method using sheath‐flow probe electrospray ionisation‐mass spectrometry for the direct identification of cocaine metabolites in dried blood spots

Kim

Kelly

Turner

et al. 2022

Rapid Comm Mass Spectrometry

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Small amounts of biofluid samples are frequently found at crime scenes, however existing gold standard methods such as LC-MS frequently require destructive extraction of the sample before a time-consuming analysis which puts strain on forensic analysis providers and can preclude further sample analysis. This study presents the application of Sheath Flow Probe Electrospray Ionization -Mass Spectrometry (sfPESI-MS) to the direct analysis of drug metabolites in dried blood spots as a high throughput, minimally destructive alternative. METHODS:A rapid direct analysis method using a sfPESI ionisation source coupled to an Orbitrap Exactive mass spectrometer was applied to detect cocaine metabolites (benzoylecgonine, BZE, cocaethylene, CE, and ecgonine methyl ester, EME) from dried blood spots. An optimisation study exploring the use of different chemical modifiers (formic acid and sodium acetate) in the sfPESI probe extraction solvent was conducted to enhance the sensitivity and reproducibility of the sfPESI-MS method. RESULTS:Optimization of the extraction solvent significantly enhanced the sensitivity and reproducibility of the sfPESI-MS method. A quantitative response over a 5-point calibration range 0.5 to 10 µg/mL was obtained for benzoylecgonine (R 2 = 0.9979) and cocaethylene (R 2 = 0.9948). Limits-of-detection (LoD) of 1.31, 0.29 and 0.15 µg/mL were achieved for EME, BZE and CE respectively from 48-hour aged dried blood spots with % RSD across the calibration range ranging between 19 -28 % for [BZE+H] + , 13 -21 % for [CE+H] + and 12 -29 % for [EME+H] + .

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Point Analysis of Foods by Sheath-Flow Probe Electrospray Ionization/Mass Spectrometry (sfPESI/MS) Coupled with a Touch Sensor

Cited by 11 publications

References 27 publications

Molecular Mapping of Sebaceous Squalene by Ambient Mass Spectrometry

Molecular Mapping of Sebaceous Squalene by Ambient Mass Spectrometry

The study of distribution of ingested terbinafine on skin with ambient ionization tandem mass spectrometry

Development of a rapid, in‐situ analysis method using sheath‐flow probe electrospray ionisation‐mass spectrometry for the direct identification of cocaine metabolites in dried blood spots

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