Point mutation in meningococcal por A gene associated with increased endemic disease

McGuinness, Brian; Clarke, Ian N.; Lambden, Paul R.; Barlow, Ann K.; Heckels, John E.; Poolman, Jan; Jones, D. M.

doi:10.1016/0140-6736(91)91297-8

Cited by 170 publications

(89 citation statements)

References 14 publications

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“…Other impressive results were obtained by Musser and co-workers [17], who demonstrated that in the United States a single clone of unusually high virulence is responsible in major part for the high morbidity and mortality caused by GBS type III organisms. A similar phenomenon was described recently by McGuinness and colleagues [18].…”

Section: Resultssupporting

confidence: 85%

Sialic acid content and surface hydrophobicity of group B streptococci

et al. 1993

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SUMMARYThe sialic acid content and the cell-surface hydrophobicity index of 40 group B streptococci (GBS) strains were assessed. GBS isolated from invasive infections (virulent strains) presented an increased level of sialic acid content (14%) when compared with GBS isolated from asymptomatic patients (0 53 %). Treatment of GBS strain 85634 with neuraminidase resulted in a decrease (about 25%) in the net negative surface charge as assessed by cell electrophoresis. This finding suggests that sialic acid residues are important anionogenic groups exposed on GBS cell surface. N-acetylneuraminic acid was the only sialic acid derivative characterized in the strain 85634 as evaluated by gas-liquid chromatography. GBS from different serotypes presented a hydrophobic index mean value of 0-9. Even though the sialic acid contributed effectively to the negative charge on GBS cell surface, no difference was observed in the hydrophobic index when virulent and avirulent strains were compared.

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Section: Resultssupporting

confidence: 85%

Sialic acid content and surface hydrophobicity of group B streptococci

et al. 1993

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“…The division of UK B15:P1.16R strains into two types (G and P) as suggested by others [12,13,24,25] is supported by this work. This differs from our earlier findings in which we were unable to differentiate Gloucester and Plymouth strains.…”

Section: Disciussionsupporting

confidence: 70%

Phylogenetic and epidemiological analysis ofNeisseria meningitidisusing DNA probes

Knight

Cartwright

et al. 1992

Epidemiol. Infect.

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SUMMARYThe genetic relationships between various serotypes and serogroups of meningococcal strains were investigated by restriction fragment-length polymorphism (RFLP) analysis using a number of random DNA probes and a probe containing a truncated copy of the meningococcal insertion sequence 11106. The data were used to estimate genetic distance between all pairs of strains and to construct phylogenetic trees for meningococcal strains. B15:P1.16R strains isolated from cases of systemic meningococcal disease in two health districts with a high incidence of disease were clonal in contrast to similar strains from cases occurring in other parts of the UK. Strains from these areas, which contain a similar genomic deletion. were found to be derived from two distinct lineages within the B15: P1. 16R phylogenetic group. RFLP data demonstrated that present serological typing systems for the meningoccus do not necessarily reflect true genetic relationships.

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“…Perhaps these differences could be attributed to a point mutation as described by Tenover et al [7]. If this genetic event had affected antigenic determinants, such as in class 1 protein, it would have generated a more virulent variant as described by McGuinness et al [13].…”

Section: Discussionmentioning

confidence: 99%

Investigation for a more virulent variant among the C:2b:P1.2,5 Spanish meningococcal epidemic strains by molecular epidemiology

Arreaza¹,

Berrón²,

Fernández

et al. 2000

Journal of Medical Microbiology

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A rise in the incidence of meningococcal disease has occurred in Spain in recent years, especially in some regions in the north-west of the country. Most cases have been caused by meningococci characterised as Neisseria meningitidis C:2b:P1.2,5. A total of 107 C:2b:P1.2,5 meningococcal isolates (60 from patients and 47 from carriers) and 12 isolates showing related antigenic combinations (C:2b:NST, C:2b:P1.2, C:2b:P1.5, C:NT:P1.2,5) was analysed by pulsed-®eld gel electrophoresis to determine the genetic variability of the epidemic and related strains. Endonucleases Bgl II and Nhe I were used to cut chromosomal DNA. When Bgl II was used, most of the C:2b:P1.2,5 isolates showed the same pulsotype regardless of whether they were from clinical cases or carriers. Isolates showing the principal pro®le after digestion with endonuclease Bgl II were analysed with Nhe I. Four pulsotypes were identi®ed, of which two were found in only one isolate each. The major pro®les (1 and 2) showed differential distribution among clinical and carrier isolates; pulsotype 1 was the most frequent among clinical isolates. However, the proportions of isolates showing pro®les 1 and 2 were similar among carrier isolates. This could indicate that there are two variants of the C:2b:P1.2,5 strain with differing pathogenicity.

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Point mutation in meningococcal por A gene associated with increased endemic disease

Cited by 170 publications

References 14 publications

Sialic acid content and surface hydrophobicity of group B streptococci

Sialic acid content and surface hydrophobicity of group B streptococci

Phylogenetic and epidemiological analysis ofNeisseria meningitidisusing DNA probes

Investigation for a more virulent variant among the C:2b:P1.2,5 Spanish meningococcal epidemic strains by molecular epidemiology

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