Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco

Suzuki, Masashi; Kuwata, Shigeru; Masuta, Chikara; Takanami, Yoichi

doi:10.1099/0022-1317-76-7-1791

Cited by 92 publications

(54 citation statements)

References 10 publications

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“…Using pseudorecombinants, these authors concluded that CMV RNAs 1 and 2 were involved in determining symptom severity. On the other hand, Sugiyama et al (2000) and Suzuki et al (1995) reported that the severity of systemic mosaic symptoms in tobacco was controlled by amino acids 111, 124 and 129 of the CMV CP gene on RNA 3. The unusual severe stunting and leaf blistering of N. glutinosa, but not other Nicotiana species by R-CMV was mapped to amino acid position 193 of the CP gene (Carrère et al 1999;Szilassy et al 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Maize appears to be resistant to systemic infection by most subgroup II strains (Wahyuni et al 1992;Carrère et al 1999;Ryu et al 1998). CMV sequences that control symptom type and virulence have been mapped to the 3a and CP genes on RNA 3 (Carrère et al 1999;Suzuki et al 1995;Sugiyama et al 2000;Szilassy et al 1999), but RNAs 1 and 2 have also been implicated (Roossinck and Palukaitis 1990;Zhang et al 1994;Hellwald et al 2000b;Soards et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Sulistyowati

Mitter²,

Bastiaan‐Net³

et al. 2004

Austral. Plant Pathol.

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G The first two authors contributed equally to this work.Abstract. We have characterised six Australian Cucumber mosaic virus (CMV) strains belonging to different subgroups, determined by the sequence of their complete RNA 3 and by their host range and the symptoms they cause on species in the Solanaceae, Cucurbitaceae and on sweet corn. These data allowed classification of strains into the known three CMV subgroups and identification of plant species able to differentiate the Australian strains by symptoms and host range. Western Australian strains 237 and Twa and Queensland strains 207 and 242 are closely related members of CMV subgroup IA, which cause similar severe symptoms on Nicotiana species. Strains 207 and 237 (subgroup IA) were the only strains tested which systemically infected sweet corn. Strain 243 caused the most severe symptoms of all strains on Nicotiana species, tomato and capsicum and appears to be the first confirmed subgroup IB strain reported in Australia. Based on pair-wise distance analysis and phylogeny of RNA 3, as well as mild disease symptoms on Nicotiana species, CMV 241 was assigned to subgroup II, as the previously described Q-CMV and LY-CMV.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Sulistyowati

Mitter²,

Bastiaan‐Net³

et al. 2004

Austral. Plant Pathol.

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“…As the cucumoviral 2b protein is involved in the control of symptom expression (Ding et al 1995;Soards et al 2002), replacing the 2b sequence of the CMV2-A1 vector with that of an attenuated CMV such as CM95 (Kosaka and Fukunishi 1997) would reduce the viral symptom. Also, it may be effective to use a pseudorecombinant virus consisting of CMV2-A1 and RNA 1 and/or RNA 3 of other strains of CMV, because it is known that symptom expression is also affected by RNA 1 or RNA 3 in addition to the activity of the 2b protein (Shintaku et al 1992;Suzuki et al 1995). We have found that a pseudorecombinant virus consisting of CMV2-A1 and RNA 1 and RNA 3 of the leguminous strain of CMV (CMV-L; Karasawa et al 1997) infects N. benthaminana plants without exhibiting visible symptoms ( Figure 5).…”

Section: Discussionmentioning

confidence: 99%

Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector

Otagaki

Arai

Takahashi

et al. 2006

Plant Biotechnology

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“…MP plays an important role in cell to cell movement of the virus. While coat protein is required for encapsidation of the genomic RNAs into virus particles, important in aphid transmission [7,30] and affecting symptom expression [42,49]. CMV strains are divided into two subgroups I and II [3,31,33] and subgroup I is subdivided into IA and IB based on nucleotide sequence of the 3 0 untranslated region of RNA3 [37].…”

Section: Introductionmentioning

confidence: 99%

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

et al. 2013

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Cucumber mosaic virus (CMV) has a wide host range causing severe damage in many important agricultural and ornamental crops. Earlier reports showed the prevalence of CMV subgroup I isolates in India. However, some recent reports point towards increasing incidence of subgroup II isolates in the country. The complete genome of a CMV isolate causing severe mosaic in cucumber was characterized and its phylogenetic analysis with other 21 CMV isolates reported worldwide clustered it with subgroup II strains. The genome comprised of RNA 1 (3,379 nucleotides), RNA 2 (3,038 nucleotides) and RNA 3 (2,206 nucleotides). The isolate showed highest homology with subgroup II isolates: 95.1-98.7, 87.7-98.0, and 85.4-97.1 % within RNA1, RNA2, and RNA3, respectively. RNA1 and RNA2 were closely related to the Japanese isolate while RNA3 clustered with an American isolate. Host range studies revealed that isolate showed severe mosaic symptoms on Nicotiana spp. and Cucumis spp. The isolate induced leaf deformation and mild filiform type symptoms in tomato. To best of our knowledge this is the first report of complete genome of CMV subgroup II isolate from India.

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Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco

Cited by 92 publications

References 10 publications

Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Host range, symptom expression and RNA 3 sequence analyses of six Australian strains ofCucumber mosaic virus

Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

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