Point-of-care tests: A review of advances in the emerging diagnostic tools for dengue virus infection

Darwish, Nadiya Taha; Sekaran, Shamala Devi; Khor, Sook Mei

doi:10.1016/j.snb.2017.09.159

Cited by 60 publications

(34 citation statements)

References 124 publications

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“…The generic platform in Figure 13 may be applied beyond chiral plasmonics, being a possible ingredient in a new healthcare paradigm where diagnostics and treatment are expected to be individualized, with continuous monitoring of patients' health [123][124][125][126][127][128][129][130]. In this approach, an active participation of the patient for disease management is expected via wearable and/or handheld devices, for self-assessment through information sharing with peer groups and specialized medical centers, thus reducing costs of regional and global healthcare systems [121,122].…”

Section: Discussionmentioning

confidence: 99%

Chiral Plasmonics and Their Potential for Point-of-Care Biosensing Applications

Paiva-Marques

Gómez

Oliveira

et al. 2020

Sensors

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There has been growing interest in using strong field enhancement and light localization in plasmonic nanostructures to control the polarization properties of light. Various experimental techniques are now used to fabricate twisted metallic nanoparticles and metasurfaces, where strongly enhanced chiral near-fields are used to intensify circular dichroism (CD) signals. In this review, state-of-the-art strategies to develop such chiral plasmonic nanoparticles and metasurfaces are summarized, with emphasis on the most recent trends for the design and development of functionalizable surfaces. The major objective is to perform enantiomer selection which is relevant in pharmaceutical applications and for biosensing. Enhanced sensing capabilities are key for the design and manufacture of lab-on-a-chip devices, commonly named point-of-care biosensing devices, which are promising for next-generation healthcare systems.

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Section: Discussionmentioning

confidence: 99%

Chiral Plasmonics and Their Potential for Point-of-Care Biosensing Applications

Paiva-Marques

Gómez

Oliveira

et al. 2020

Sensors

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“…Many studies have recorded the virus isolation methods as the best diagnostic method for detecting dengue infection [20][21]. In this method, the infected blood samples are cultured in a variety of cell lines of either mosquito (AP-61, Tra-284, AP64, C6/36, and CLA-1 cells) or mammalian (LLCMK2, Vero, and BHK-21 cells) origin or in live mosquitoes.…”

Section: Virus Isolationmentioning

confidence: 99%

Recent Trends in Dengue Detection Methods Using Biosensors

Zainuddin

Nordin

Rahim

2018

IIUMEJ

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Dengue illness is an infectious tropical disease, transmitted by Aedes mosquitos, that poses a serious health threat to the tropical world. This disease causes widespread infection worldwide, with about 50 million cases of dengue occurring per annum out of which 500,000 recorded cases of dengue hemorrhagic fever and 22,000 deaths. Currently, there are no effective vaccines available to prevent the spread of the infection. Accurate and rapid laboratory diagnostic tests are required for early detection to reduce patient mortality rate. In this paper, common laboratory diagnosis methods for detecting dengue virus infection are discussed. Currently, virus isolation, RT-PCR and serology methods provide the most direct and accurate response for detection of dengue. However, these methods require tedious steps, expensive requirements and expert staffs. Recent research have proposed the usage of biosensors as an alternative new technology for detection of dengue. In this work, various types of biosensors such as electrochemical, piezoelectric, and optical biosensor have been described and compared to evaluate their effectiveness in dengue detection. It is observed that the optical biosensor offers the best detection due to its high sensitivity as compared to others, although it is popularly known as an expensive method. Alternatively, the use of electrochemical and piezoelectric biosensors (QCM) is highly recommended for detection of dengue due to their ease-of-use, low cost, low reagent consumption, disposability, and minimal sample preparation. These approaches have the potential to improve the rate of survival, particularly in resource-limited countries. ABSTRAK: Virus denggi adalah penyakit berjangkit tropika bawaan nyamuk Aedes yang menimbulkan ancaman serius kepada kesihatan global. Penyakit ini menyebabkan jangkitan yang meluas di seluruh dunia, dengan kira-kira 50 juta kes denggi yang berlaku setiap tahun di mana 500,000 kes demam denggi berdarah dan 22,000 kematian direkodkan. Buat masa ini, tiada vaksin yang berkesan untuk mencegah penyebaran jangkitan ini. Ujian diagnostik makmal yang cepat dan tepat diperlukan untuk pengesanan denggi awal untuk mengurangkan kadar kematian pesakit. Dalam artikel ini, kaedah diagnosis makmal yang biasa dilakukan untuk mengesahkan jangkitan virus denggi akan dibincangkan. Pada masa kini, kaedah pengasingan virus, RT-PCR dan serologi adalah tindak balas yang paling cepat dan tepat untuk mengesan denggi. Walau bagaimanapun, kaedah-kaedah ini memerlukan langkah-langkah yang melecehkan, kos penyelenggaraan yang tinggi dan kakitangan yang terlatih. Penyelidikan terkini telah mencadangkan penggunaan biosensor sebagai teknologi baru alternatif untuk mengesan denggi. Dalam artikel ini juga pelbagai jenis biosensor seperti biosensor elektrokimia, piezoelektrik, dan biosensor optik telah dijelaskan dan dibandingkan untuk menilai keberkesanannya dalam pengesanan denggi. Difahamkan bahawa biosensor optik menawarkan pengesanan terbaik kerana kepekaannya yang tinggi berbanding dengan yang lain, walaupun ia dikenali sebagai kaedah berkos tinggi. Sebaliknya, biosensor elektrokimia dan piezoelektrik (QCM) sangat disyorkan untuk mengesan denggi kerana ia mudah digunakan, berkos rendah, penggunaan bahan uji yang terhad, boleh dipakai buang, dan mempunyai penyediaan sampel yang minima. Pendekatan-pendekatan ini berpotensi untuk meningkatkan kadar kemandirian di kawasan-kawasan sumber terhad.

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“…Initial work overwhelmingly focused on chip-based analytical separations, e.g., capillary electrophoresis (CE) or high-performance liquid chromatography (HPLC), and the miniaturization of individual fluidic components such as pumps and valves through micromachining. In the recent decade there have been increasing efforts to apply 'lab-on-a-chip' to automating existing laboratory processes and to enable point-of-care/point-of-use distributed testing [6] and to leverage microfluidic physical effects to develop entirely new medical tools such as liquid biopsy [7] and exosome-based diagnostics [8].…”

Section: Introductionmentioning

confidence: 99%

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

Burger¹,

Kinahan

Cayron

et al. 2019

Inventions

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We present a powerful and compact batch-mode mixing and dilution technique for centrifugal microfluidic platforms. Siphon structures are designed to discretize continuous flows into a sequence of droplets of volumes as low as 100 nL. Using a passive, self-regulating 4-step mechanism, discrete volumes of two fluids are alternatingly issued into a common intermediate chamber. At its base, a capillary valve acts as a fluidic shift register; a single droplet is held in place while two or more droplets merge and pass through the capillary stop. These merged droplets are advectively mixed as they pass through the capillary valve and into the receiving chamber. Mixing is demonstrated for various combinations of liquids such as aqueous solutions as well as saline solutions and human plasma. The mixing quality is assessed on a quantitative scale by using a colorimetric method based on the mixing of potassium thiocyanate and iron(III) chloride, and in the case of human plasma using a spectroscopic method. For instance, volumes of 5 µL have been mixed in less than 20 s. Single-step dilutions up to 1:5 of plasma in a standard phosphate buffer solution are also demonstrated. This work describes the preliminary development of the mixing method which has since been integrated into a commercially available microfluidic cartridge.Inventions 2020, 5, 1 2 of 14 microfluidic systems constitute a subset which uses the rotation of the chip, which is typically of a similar geometry as a Compact Disc™ or DVD™, to enable most pumping and valving operations. These systems can be further sub-divided into 'Bioanalytical Screening CDs' and 'Lab-on-a-Disc (LoaD)'. Bioanalytical screening CDs focus on adapting optical disc drive (ODD) technology (e.g., Compact Disc, DVD) for biological detection [9]. This technology takes advantage of the high-density data storage capability as well as the favorable pricing for its main constituents, i.e., the optical pickup unit, the spindle drive, and the optical disc substrates [10][11][12][13][14].The LoaD [15][16][17][18][19][20] is typically used to automate the standard laboratory steps conducted in a biology lab to enable a fully automated sample-to-answer system. The LoaD is particularly applicable for deployment in the field (point-of-use) or at the hospital bed-side (point-of-care) as the chips can be loaded at atmospheric pressure (via pipette or syrette) without issues regarding sealing or priming. The use of centrifugation for pumping also reduces the cost and complexity of support instrumentation as just low-cost spindle motors are required rather than specialized micropumps. Similarly, inherent centrifugation of samples [21][22][23] applied to sample preparation is a particular strength.The technological precursor of these systems are the centrifugal analyzers which became popular in the 1970s and 1980s [24] for automating a very limited number of simple assay steps, on rather macroscopic sample volumes, and readout on a rotor-based instrument. Currently the main areas of application are immuno...

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Point-of-care tests: A review of advances in the emerging diagnostic tools for dengue virus infection

Cited by 60 publications

References 124 publications

Chiral Plasmonics and Their Potential for Point-of-Care Biosensing Applications

Chiral Plasmonics and Their Potential for Point-of-Care Biosensing Applications

Recent Trends in Dengue Detection Methods Using Biosensors

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

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