Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures

Cerruti, Benedetta; Puliafito, Alberto; Shewan, Annette M.; Yu, Wei; Combes, Alexander N.; Little, Melissa H.; Chianale, Federica; Primo, Luca; Serini, Guido; Mostov, Keith E.; Celani, Antonio; Gamba, A.

doi:10.1083/jcb.201305044

Cited by 48 publications

(42 citation statements)

References 60 publications

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“…Three‐dimensional fluorescence imaging of the SS75‐cleared tumor tissue stained with DAPI was also successfully performed (Figure ). DAPI imaging in tissues is quite useful for various applications, such as analysis of apoptosis, detection of neurons in brain and retina, and imaging of tubular lumen structure in some organs . Our result suggests that DNA was not affected by the poly(AAm‐co‐SS) hydrogel.…”

Section: Resultsmentioning

confidence: 72%

“…DAPI imaging in tissues is quite useful for various applications, such as analysis of apoptosis, detection of neurons in brain and retina, and imaging of tubular lumen structure in some organs. [22][23][24][25][26][27] Our result suggests that DNA was not affected by the poly(AAm-co-SS) hydrogel. However, there is concern that proteins might be affected by the polymer, because some previous reports showed protein denaturation by poly(sodium p-styrenesulfonate).…”

Section: Optical Tissue Clearingmentioning

confidence: 63%

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Rapid optical tissue clearing using poly(acrylamide‐co‐styrenesulfonate) hydrogels for three‐dimensional imaging

et al. 2019

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Optical tissue clearing methods are attractive for three‐dimensional imaging of intact tissues and organs. A CLARITY (clear lipid‐exchanged acrylamide‐hybridized rigid imaging/immunostaining/in situ‐hybridization‐compatible tissue‐hydrogel) method using polyacrylamide gels was recently developed. In the current study, we used poly(acrylamide‐co‐styrenesulfonate) gels as a polyelectrolyte gel for a passive CLARITY method. First, radical copolymerization of acrylamide (AAm) and sodium p‐styrenesulfonate (SS) at different ratios was performed. The composition of the copolymer could be controlled by changing the monomer ratio. With increased SS content, the molecular weight decreased and the refractive index slightly increased. The poly(AAm‐co‐SS) hydrogels with a higher SS content were more swollen and looser. The clearing time could be reduced using the poly(AAm‐co‐SS) hydrogel with a higher SS content. Three‐dimensional fluorescence imaging of the poly(AAm‐co‐SS) hydrogel‐cleared tissues was successfully performed after treatment using a blue fluorescent DNA stain. Therefore, the use of the poly(AAm‐co‐SS) hydrogel improved the clearing process in the passive CLARITY method, and the technique is useful for fluorescence imaging of DNA. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2297–2304, 2019.

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Section: Resultsmentioning

confidence: 72%

Section: Optical Tissue Clearingmentioning

confidence: 63%

Rapid optical tissue clearing using poly(acrylamide‐co‐styrenesulfonate) hydrogels for three‐dimensional imaging

et al. 2019

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“…Three-dimensional model systems have already been used to study the formation of nondental cysts, such as in kidney disease, using the Madin-Darby canine kidney (MDCK) cell line (Pey et al 1996, Cerruti et al 2013. For example, fibroblast cells may be added to the collagen matrix to investigate the epithelial-mesenchymal interactions and collagen degradation, which may affect epithelial cell spheroid behaviour and modify cell survival, proliferation and migration.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, mathematical models have been used to develop complementary theories of cyst development (Ward et al 2004, Cerruti et al 2013. However, these mathematical models do not explain why not all periapical lesions progress into radicular cysts.…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro model to study tooth cystogenesis

et al. 2019

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Aim To describe an in vitro experimental model of cystic structure formation to conduct research on radicular cyst development. Methodology To form spheroid structures, various numbers (1 × 104, 5 × 104 or 1 × 105) of epithelial cells (HaCaT and Cal27) were seeded in 96‐well plates previously coated with 1.5% low‐melting agarose. After 24 h, the spheroids were collected, embedded in 3D collagen matrix and transferred to 24‐well plates previously coated with polymerized collagen and kept for up to 21 days. Images of spheroids were captured at each time‐point (1, 5, 9, 15 and 21 days), and samples underwent histological and confocal microscopy analyses. Spheroid area, perimeter and cell dispersion were measured. One‐way Anova was used for statistical analysis. Results Both epithelial cell lines were able to generate regular and circular spheroids after 24 h of incubation regardless of cell density. Spheroid structures in the collagen matrix were uniform in most samples until day 15, when several spots that appeared to be new cultures were seen. Spheroids from HaCaT were significantly more stable than those from Cal27 (P < 0.05). Starting on the third day, the examination of histological sections revealed a cavity with epithelial lining morphology, similar to a pathological radicular cyst. Conclusions This study describes an experimental model of cystogenesis in vitro that may be used to test theories and investigates the effects of different growth factors during cyst development and maintenance.

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“…Furthermore, microlumens have been observed at the basolateral surface of polarized MDCK cysts, possibly forming after cell division, and these coalesce to form the lumen (Cerruti et al 2013). How PIs contribute to this process is not understood.…”

Section: Pis Apicobasal Polarity and Lumen Formationmentioning

confidence: 99%

Cell Polarity 1

Ebnet¹

2015

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Polarity, cell division, and out-of-equilibrium dynamics control the growth of epithelial structures

Abstract: Mathematical modeling and analysis of 3D epithelial structures indicate that epithelial growth can take place far from mechanical equilibrium, depending on cell–cell and cell–ECM contact, cell division, cortical contractility, and cell motility.

Cited by 48 publications

References 60 publications

Rapid optical tissue clearing using poly(acrylamide‐co‐styrenesulfonate) hydrogels for three‐dimensional imaging

Rapid optical tissue clearing using poly(acrylamide‐co‐styrenesulfonate) hydrogels for three‐dimensional imaging

Development of an in vitro model to study tooth cystogenesis

Cell Polarity 1

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