Polarization control of metal-enhanced fluorescence in hybrid assemblies of photosynthetic complexes and gold nanorods

Bujak, Łukasz; Olejnik, Maria; Brotosudarmo, Tatas Hardo Panintingjati; Schmidt, Mikołaj K.; Czechowski, Nikodem; Piatkowski, Dawid; Aizpurua, Javier; Cogdell, Richard J.; Heiß, Wolfgang; Maćkowski, Sebastian

doi:10.1039/c3cp54364a

Cited by 15 publications

(8 citation statements)

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“…By tuning the spacing between the molecules and metallic substrate, coupling effect between SPR and excited state molecules can be moderated [84]. Recently, several works have reported that the result of investigations of the distance and plasmon wavelength dependent fluorescence of fluorophore via nanorod or nanoparticle substrate [85,86]. In particular, core-shell configuration, precisely controlling the distance between fluorophore and plasmonic nanostructure, have been widely used in the investigation of PEF effect recently [87][88][89][90].…”

Section: The Wavelength and Spacer Effect Towards The Fluorescence Enmentioning

confidence: 99%

Recent Progress on Plasmon-Enhanced Fluorescence

et al. 2015

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Abstract:The optically generated collective electron density waves on metal-dielectric boundaries known as surface plasmons have been of great scientific interest since their discovery. Being electromagnetic waves on gold or silver nanoparticle's surface, localised surface plasmons (LSP) can strongly enhance the electromagnetic field. These strong electromagnetic fields near the metal surfaces have been used in various applications like surface enhanced spectroscopy (SES), plasmonic lithography, plasmonic trapping of particles, and plasmonic catalysis. Resonant coupling of LSPs to fluorophore can strongly enhance the emission intensity, the angular distribution, and the polarisation of the emitted radiation and even the speed of radiative decay, which is so-called plasmon enhanced fluorescence (PEF). As a result, more and more reports on surface-enhanced fluorescence have appeared, such as SPASER-s, plasmon assisted lasing, single molecule fluorescence measurements, surface plasmoncoupled emission (SPCE) in biological sensing, optical orbit designs etc. In this review, we focus on recent advanced reports on plasmon-enhanced fluorescence (PEF). First, the mechanism of PEF and early results of enhanced fluorescence observed by metal nanostructure will be introduced. Then, the enhanced substrates, including periodical and nonperiodical nanostructure, will be discussed and the most important factor of the spacer between molecule and surface and wavelength dependence on PEF is demonstrated. Finally, the recent progress of tipenhanced fluorescence and PEF from the rare-earth doped up-conversion (UC) and down-conversion (DC) nanoparticles (NPs) are also commented upon. This review provides an introduction to fundamentals of PEF, illustrates the current progress in the design of metallic nanostructures for efficient fluorescence signal amplification that utilises propagating and localised surface plasmons.

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Section: The Wavelength and Spacer Effect Towards The Fluorescence Enmentioning

confidence: 99%

Recent Progress on Plasmon-Enhanced Fluorescence

et al. 2015

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“…The former is challenging for our set-up to collect sufficient signal and the latter would be highly technically challenging requiring super-resolution detection of native chromophores (rather than idealized fluorophores designed for super-resolution applications). We have shown proof-of-concept for the utility of nanoscale topography and optical detection of low-number of complexes for observing NPQ switching, we suggest that extending this combinatorial approach to also incorporate metal nanoparticles for localized enhancement of excitation or emission [ 70 ] could allow the ultimate in vitro platform for mapping how NPQ changes and exciton dynamics change within native-like membrane environments with single-protein optical and topographic resolution.…”

Section: Discussionmentioning

confidence: 99%

Correlated fluorescence quenching and topographic mapping of Light-Harvesting Complex II within surface-assembled aggregates and lipid bilayers

Adams

Vasilev

Hunter

et al. 2018

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Light-Harvesting Complex II (LHCII) is a chlorophyll-protein antenna complex that efficiently absorbs solar energy and transfers electronic excited states to photosystems I and II. Under excess light intensity LHCII can adopt a photoprotective state in which excitation energy is safely dissipated as heat, a process known as Non-Photochemical Quenching (NPQ). In vivo NPQ is triggered by combinatorial factors including transmembrane ΔpH, PsbS protein and LHCII-bound zeaxanthin, leading to dramatically shortened LHCII fluorescence lifetimes. In vitro, LHCII in detergent solution or in proteoliposomes can reversibly adopt an NPQ-like state, via manipulation of detergent/protein ratio, lipid/protein ratio, pH or pressure. Previous spectroscopic investigations revealed changes in exciton dynamics and protein conformation that accompany quenching, however, LHCII-LHCII interactions have not been extensively studied. Here, we correlated fluorescence lifetime imaging microscopy (FLIM) and atomic force microscopy (AFM) of trimeric LHCII adsorbed to mica substrates and manipulated the environment to cause varying degrees of quenching. AFM showed that LHCII self-assembled onto mica forming 2D-aggregates (25–150 nm width). FLIM determined that LHCII in these aggregates were in a quenched state, with much lower fluorescence lifetimes (~0.25 ns) compared to free LHCII in solution (2.2–3.9 ns). LHCII-LHCII interactions were disrupted by thylakoid lipids or phospholipids, leading to intermediate fluorescent lifetimes (0.6–0.9 ns). To our knowledge, this is the first in vitro correlation of nanoscale membrane imaging with LHCII quenching. Our findings suggest that lipids could play a key role in modulating the extent of LHCII-LHCII interactions within the thylakoid membrane and so the propensity for NPQ activation.

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“…Previous studies of the interactions of gold or silver NPs with photosynthetic proteins have focussed in the main on the photophysical consequences, including enhanced absorption and energy transfer. [80][81][82][83][84][85][86][87][88][89][90][91][92] A smaller number of studies have reported the impact of plasmonic interactions on photocurrents from photosynthetic materials on electrodes or in cells. Enhancements have been seen after placing Photosystem I complexes on a silver island film 93 and decorating thylakoid membranes with gold nanorods.…”

Section: Discussionmentioning

confidence: 99%