Polarization Microscopy

Inoué, Shinya

doi:10.1002/0471143030.cb0409s13

Cited by 14 publications

(7 citation statements)

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“…For a more in-depth discussion of the technique of polarized light microscopy and its application in living cells and organisms, we refer to the many lucid articles published by Inou e and coworkers, including the recent publication of Inou e's collected works (Inou e, 1986(Inou e, , 2003(Inou e, , 2008Inoue and Oldenbourg, 1998;Inoue, 2002). For a practical guide on using the LC-PolScope and technical aspects of polarized light microscopy, we refer to the following publications: Oldenbourg, 1999Oldenbourg, , 2005Oldenbourg, , 2007Shribak and Oldenbourg, 2003;Oldenbourg and Shribak, 2010.…”

Section: Further Readingmentioning

confidence: 99%

Polarized light microscopy in reproductive and developmental biology

Koike-Tani

Tani

Mehta

et al. 2013

Molecular Reproduction Devel

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SUMMARY The polarized light microscope reveals orientational order in native molecular structures inside living cells, tissues, and whole organisms. It is a powerful tool used to monitor and analyze the early developmental stages of organisms that lend themselves to microscopic observations. In this article, we briefly discuss the components specific to a traditional polarizing microscope and some historically important observations on: chromosome packing in the sperm head, the first zygote division of the sea urchin, and differentiation initiated by the first asymmetric cell division in the sand dollar. We then introduce the LC-PolScope and describe its use for measuring birefringence and polarized fluorescence in living cells and tissues. Applications range from the enucleation of mouse oocytes to analyzing the polarized fluorescence of the water strider acrosome. We end with new results on the birefringence of the developing chick brain, which we analyzed between developmental stages of days 12–20.

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Section: Further Readingmentioning

confidence: 99%

Polarized light microscopy in reproductive and developmental biology

Koike-Tani

Tani

Mehta

et al. 2013

Molecular Reproduction Devel

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“…Inspired by Schmidt's observations, Shinya Inoué significantly improved the sensitivity of the instrument and made seminal contributions to our understanding of the architectural dynamics inside living cells (Inoue et al 1975; Inoué and Hyde 1957; Inoue and Sato 1967). For many lucid discussions of polarized light microscopy and its application to biology, we refer the reader to the many articles and books by Inoué, including his recently published Collected Works (Inoue 2002; Inoué 1986; Inoué 2003; Inoué 2008; Inoue and Oldenbourg 1998). Tinoco and collaborators reviewed the imaging of dichroism and diattenuation in biological materials (Tinoco et al 1987).…”

Section: Further Readingsmentioning

confidence: 99%

Polarized light imaging of birefringence and diattenuation at high resolution and high sensitivity

Mehta

Shribak

Oldenbourg

2013

J. Opt.

108

103

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Polarized light microscopy provides unique opportunities for analyzing the molecular order in man-made and natural materials, including biological structures inside living cells, tissues, and whole organisms. 20 years ago, the LC-PolScope was introduced as a modern version of the traditional polarizing microscope enhanced by liquid crystal devices for the control of polarization, and by electronic imaging and digital image processing for fast and comprehensive image acquisition and analysis. The LCPolScope is commonly used for birefringence imaging, analyzing the spatial and temporal variations of the differential phase delay in ordered and transparent materials. Here we describe an alternative use of the LC-PolScope for imaging the polarization dependent transmittance of dichroic materials. We explain the minor changes needed to convert the instrument between the two imaging modes, discuss the relationship between the quantities measured with either instrument, and touch on the physical connection between refractive index, birefringence, transmittance, diattenuation, and dichroism.

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“…Polarization microscopy is a familiar method in visible optics that has many applications in, e.g., geoscience for the identification of minerals [1], in biology for the analysis of the organization of cells and tissues [2], and in material science for residual stress measurements [3]. It is used to reveal the connection between the structure of the material and its properties and mode of action.…”

Section: Introductionmentioning

confidence: 99%

Scanning high-sensitive x-ray polarization microscopy

et al. 2022

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We report on the realization of an extremely sensitive X-ray polarization microscope, allowing to detect tiniest polarization changes of 1 in 100 billion (10-11) with a μm-size focused beam. The extreme degree of polarization purity places the most stringent requirements on the orientation of the polarizer and analyzer crystals as well as the composition and the form fidelity of the lenses, which must not exhibit any birefringence. The results show that these requirements are currently only met by polymer lenses. Highly sensitive scanning X-ray polarization microscopy thus is established as a new method. It can provide new insights in a wide range of applications ranging from quantum electrodynamics and quantum optics to X-ray spectroscopy, materials research, and laser physics.

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Polarization Microscopy

Cited by 14 publications

References 61 publications

Polarized light microscopy in reproductive and developmental biology

Polarized light microscopy in reproductive and developmental biology

Polarized light imaging of birefringence and diattenuation at high resolution and high sensitivity

Scanning high-sensitive x-ray polarization microscopy

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