2020
DOI: 10.3347/kjp.2020.58.3.217
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Polarization of M2 Macrophages by Interaction between Prostate Cancer Cells Treated with Trichomonas vaginalis and Adipocytes

Abstract: Trichomonas vaginalis causes inflammation of the prostate and has been detected in tissues of prostate cancers (PCa), prostatitis and benign prostatic hyperplasia. Obesity is a risk factor for PCa and causes a chronic subclinical inflammation. This chronic inflammation further exacerbates adipose tissue inflammation as results of migration and activation of macrophages. Macrophages are the most abundant immune cells in the PCa microenvironment. M2 macrophages, known as Tumor-Associated Macrophages, are… Show more

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Cited by 10 publications
(12 citation statements)
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“…Trichomonas vaginalis isolate T016 was grown in trypticase-yeast extract-maltose medium supplemented with 10% heat-inactivated horse serum (Life Technologies, Carlsbad, California, USA). The mouse prostate cancer cell line (TRAMP-C2 cells) was a gift from Prof. Jung Han Yoon Park (Hallym University) and were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; GE Healthcare Life Sciences, Logan, Utah, USA) containing penicillin and streptomycin (WelGENE, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences) in a 5% CO 2 humidified incubator at 37°C [ 11 ]. The mouse macrophage cell line (J774A.1 cells) was purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea) and cultured in DMEM containing 1% penicillin and streptomycin plus 10% FBS.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Trichomonas vaginalis isolate T016 was grown in trypticase-yeast extract-maltose medium supplemented with 10% heat-inactivated horse serum (Life Technologies, Carlsbad, California, USA). The mouse prostate cancer cell line (TRAMP-C2 cells) was a gift from Prof. Jung Han Yoon Park (Hallym University) and were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; GE Healthcare Life Sciences, Logan, Utah, USA) containing penicillin and streptomycin (WelGENE, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences) in a 5% CO 2 humidified incubator at 37°C [ 11 ]. The mouse macrophage cell line (J774A.1 cells) was purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea) and cultured in DMEM containing 1% penicillin and streptomycin plus 10% FBS.…”
Section: Methodsmentioning
confidence: 99%
“…The cells were washed immediately and incubated in 10% conditioned medium for 24 hr. Before and after incubation, at least 10 different fields of the wounded area of each sample were photographed with an inverted microscope (Leica, Wetzler, Germany) [ 11 ].…”
Section: Methodsmentioning
confidence: 99%
“…The cells were then cultured in DMEM containing 10% FBS and insulin for another 2 days, after which they were grown in DMEM containing 10% FBS. Lipid droplets in the cells were detected by oil-red-O staining [ 28 ].…”
Section: Methodsmentioning
confidence: 99%
“…Chemotaxis was assessed using 24-well plates fitted with 5 mm pore polycarbonate membrane inserts. The chemotactic index was calculated as described in our previous study [ 28 ]. Briefly, the lower wells were filled with 350 ml of TCM, and polycarbonate membrane inserts were placed over the lower wells.…”
Section: Methodsmentioning
confidence: 99%
“…Trichomonas vaginalis ( T. vaginalis , T016 isolate), TRAMP-C2 cell line (established from a prostate tumor of a TRAMP mouse) were cultured as described in our previous study. All the cells were incubated in 5% CO 2 at 37°C [ 19 ].…”
Section: Methodsmentioning
confidence: 99%