Polarization of the Golgi apparatus and the microtubule-organizing center in cultured fibroblasts at the edge of an experimental wound.

Kupfer, Abraham; Louvard, Daniel; Singer, S. J.

doi:10.1073/pnas.79.8.2603

Cited by 376 publications

(297 citation statements)

References 25 publications

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“…Thus microtubules may be considered as sensors of directional changes that help maintain the centrosome in a position near the cell center where the microtubule system can respond quickly to rapid and sometimes gross changes in cell morphology in the course of cell movement. This may be important for the transport of secretory vesicles from the pericentrosomal Golgi apparatus to the leading edge (8,(38)(39)(40). Microtubules do not seem to serve, however, as effectors of directional cues generated by centrosomal repositioning.…”

Section: Resultsmentioning

confidence: 99%

Centrosome positioning and directionality of cell movements

Ueda¹,

Gräf²,

MacWilliams³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

132

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In several cell types, an intriguing correlation exists between the position of the centrosome and the direction of cell movement: the centrosome is located behind the leading edge, suggesting that it serves as a steering device for directional movement. A logical extension of this suggestion is that a change in the direction of cell movement is preceded by a reorientation, or shift, of the centrosome in the intended direction of movement. We have used a fusion protein of green f luorescent protein (GFP) and ␥-tubulin to label the centrosome in migrating amoebae of Dictyostelium discoideum, allowing us to determine the relationship of centrosome positioning and the direction of cell movement with high spatial and temporal resolution in living cells. We find that the extension of a new pseudopod in a migrating cell precedes centrosome repositioning. An average of 12 sec elapses between the initiation of pseudopod extension and reorientation of the centrosome. If no reorientation occurs within approximately 30 sec, the pseudopod is retracted. Thus the centrosome does not direct a cell's migration. However, its repositioning stabilizes a chosen direction of movement, most probably by means of the microtubule system.

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Section: Resultsmentioning

confidence: 99%

Centrosome positioning and directionality of cell movements

Ueda¹,

Gräf²,

MacWilliams³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

132

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“…Furthermore, we aimed to develop a cell line which could potentially act as a chemotactic gradient sensor to assist in characterization of the tumor microenvironment and metastatic process. Prior work by others has demonstrated that during in vitro wound closure involving either normal rat kidney, endothelial or rat embryonic cells, the Golgi complex (21,27,28), perhaps in close association with the microtubule-organizing center (27,29), precedes the nucleus during directed cell migration. However, not all studies are in agreement with these findings (30), and the orientation of the Golgi with respect to the nucleus may be both cell type and microenvironment specific.…”

Section: Discussionmentioning

confidence: 99%

Monomeric red fluorescent proteins with a large Stokes shift

Piatkevich

Hulit

Subach

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

150

193

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Two-photon microscopy has advanced fluorescence imaging of cellular processes in living animals. Fluorescent proteins in the blue-green wavelength range are widely used in two-photon microscopy; however, the use of red fluorescent proteins is limited by the low power output of Ti-Sapphire lasers above 1,000 nm. To overcome this limitation we have developed two red fluorescent proteins, LSS-mKate1 and LSS-mKate2, which possess large Stokes shifts with excitation/emission maxima at 463∕624 and 460∕605 nm, respectively. These LSS-mKates are characterized by high pH stability, photostability, rapid chromophore maturation, and monomeric behavior. They lack absorbance in the green region, providing an additional red color to the commonly used red fluorescent proteins. Substantial overlap between the two-photon excitation spectra of the LSS-mKates and blue-green fluorophores enables multicolor imaging using a single laser. We applied this approach to a mouse xenograft model of breast cancer to intravitally study the motility and Golgi-nucleus alignment of tumor cells as a function of their distance from blood vessels. Our data indicate that within 40 μm the breast cancer cells show significant polarization towards vessels in living mice.cell polarity | intravital imaging | Keima | two-photon microscopy | mKate

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“…This reorientation is closely coordinated with the reorientation of Golgi apparatus to the front of the nucleus. Both events occur rapidly after cell polarization (Etienne-Manneville and Hall, 2001;Gotlieb et al, 1981;Gundersen and Bulinski, 1988;Kupfer et al, 1983;Kupfer et al, 1982). In the cell rear, the increased PTEN activity is correlated with the activity of myosin II -an actin-dependent molecular motor that ensures the trailing edge contractility and retractive behavior.…”

Section: Basic Principles Of Cell Migration Overall Structure Of the mentioning

confidence: 99%

Cell biology of embryonic migration

Kurosaka¹,

Kashina²

2008

Birth Defects Research Pt C

117

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Cell migration is an evolutionarily conserved mechanism that underlies the development and functioning of uni-and multicellular organisms and takes place in normal and pathogenic processes, including various events of embryogenesis, wound healing, immune response, cancer metastases, and angiogenesis. Despite the differences in the cell types that take part in different migratory events, it is believed that all of these migrations occur by similar molecular mechanisms, whose major components have been functionally conserved in evolution and whose perturbation leads to severe developmental defects. These mechanisms involve intricate cytoskeleton-based molecular machines that can sense the environment, respond to signals, and modulate the entire cell behavior. A big question that has concerned the researchers for decades relates to the coordination of cell migration in situ and its relation to the intracellular aspects of the cell migratory mechanisms. Traditionally, this question has been addressed by researchers that considered the intra-and extracellular mechanisms driving migration in separate sets of studies. As more data accumulate researchers are now able to integrate all of the available information and consider the intracellular mechanisms of cell migration in the context of the developing organisms that contain additional levels of complexity provided by extracellular regulation. This review provides a broad summary of the existing and emerging data in the cell and developmental biology fields regarding cell migration during development.

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Polarization of the Golgi apparatus and the microtubule-organizing center in cultured fibroblasts at the edge of an experimental wound.

Cited by 376 publications

References 25 publications

Centrosome positioning and directionality of cell movements

Centrosome positioning and directionality of cell movements

Monomeric red fluorescent proteins with a large Stokes shift

Cell biology of embryonic migration

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