Polarographic Determination of Oxazepam

Characterisation of oxazepam degradation products by high‐performance liquid chromatography/electrospray ionisation mass spectrometry and electrospray ionisation quadrupole time‐of‐flight tandem mass spectrometry

“…Various methods have been described for the determination of oxazepam14 such as UV spectrophotometric determination after extraction with ethanol. Polarography15, 16 has been used in order to increase the selectivity of analysis since it is dependent on reduction of the intact 4,5‐azomethine bond. Chromatographic methods have been widely used for oxazepam analysis.…”

Organic polarography

“…The reduction mechanism of cis and trans isomers of 1,2-dibromoethylene was shown to be the same but the rate of reduction of the trans isomer was faster (604) Reduction of 1,1,-2.2-tetrabromoethane produced two waves in 50% alcohol yielding as-dibromoethylene (231) while (CH3)2CX-N02, where X = Br, Cl, and H, yielded 2.3-dimethyl-2,3-dinitrobutane in aprotic solvents (21). An irreversible reduction process was reported for 2acetyl-5-bromothiophene in 8% ethanol at pH 2 to 5 (228). Data for dibromoethylene and dibromoethane at the DME was compared to data of dropping gallium electrode (204) Influence of the steric position of Br atom (Br in exo position reduced more easily than Br in endo position) on half-wave potentials and reversibility in reduction studies of bromo derivatives of 7-oxabicyclo(2,2,l)heptane (121) was considered.…”

“…Analytical procedures were de-veloped in ac polarographic studies of warfarin (477), nicotinic acid esters and polyethylene glycol fatty alcohol ethers (561), and of 4,4'-diacetoxydiphenyl-2pyridylmethane (870). Oxazepam (7-chloro-l,3,dihydro-3hydroxy-5-phenyl-2#-l ,4benzodiazepin-2-one) was studied electrochemically as a function of pH, reduction products were identified, and procedures for its analysis were developed (228,678,680,681,950). Two reduction waves were reported for nitrazepam (a nitro derivative of oxazepam (679).…”

Stability-Indicating High-Performance Liquid Chromatographic Assay for Oxazepam Tablets and Capsules

“…Increased selectivity was observed using polarographic detection (2,3), which is dependent on reduction of the intact 4,5-azomethine bond. The current official method (1) employs UV spectrophotometric determination after extraction with ethanol.…”