Polianthes tuberosa-Mediated Silver Nanoparticles from Flower Extract and Assessment of Their Antibacterial and Anticancer Potential: An In Vitro Approach

Abstract

Plant-mediated metallic nanoparticles have beenreported for a diversified range of applications in biological sciences. In the present study, we propose the Polianthes tuberosa flower as a reducing and stabilizing agent for the synthesis of silver nanoparticles (PTAgNPs). The PTAgNPs were exclusively characterized using UV–Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), atomic force microscopy, zeta potential, and transmission electron microscopy (TEM) studies. In a biological assay, we investigated the antibacterial and anticancer activity of silver nanoparticles in the A431 cell line. The PTAgNPs demonstrated a dose-dependent activity in E. coli and S. aureus, suggesting the bactericidal nature of AgNPs. The PTAgNPs exhibited dose-dependent toxicity in the A431 cell line, with an IC50 of 54.56 µg/mL arresting cell growth at the S phase, as revealed by flow cytometry analysis. The COMET assay revealed 39.9% and 18.15 severities of DNA damage and tail length in the treated cell line, respectively. Fluorescence staining studies indicate that PTAgNPs cause reactive oxygen species (ROS) and trigger apoptosis. This research demonstrates that synthesized silver nanoparticles have a significant effect on inhibiting the growth of melanoma cells and other forms of skin cancer. The results show that these particles can cause apoptosis or cell death in malignant tumor cells. This suggests that they could be used to treat skin cancers without harming normal tissues.

Bio‐fabrication and characterization of nano‐silver using Kickxia elatine plant and its anti‐diabetic effect on alloxan‐induced Sprague Dawley rats — An in vivo approach

“…These phyto‐compounds are involved in reduction of metal ions to NPs 91 . The FTIR finding is supported by previous literature, 92,93 The interaction between KE extract and KE‐AgNPs is indicated by a considerable decrease in intensity in the FTIR spectra of KE‐AgNPs. Therefore, it can be assumed from the IR spectra that these biomolecules have a role in both the bio reduction and the stabilization of nano‐silver 94 …”

Carthamidin-mediated silver nanoparticles synthesis, characterization and anticancer activity-Invitro Approach

“…Our recent studies of copper oxide and silver nanoparticles from Beta vulgaris and Polianthes tuberosa proved that nanoparticles arrest the cell cycle due to the induction of apoptosis. [38][39]. In the study, we annotate the response of CTAgNPs in MCF 7 cells by ow cytometry analysis.…”