Pollen and seed fertility of three functional male-sterile lines of eggplant with the wild Solanum cytoplasms

Hasnunnahar, Mst.; Khan, Md. Mizanur Rahim; Isshiki, Shiro

doi:10.1016/j.scienta.2012.03.004

Cited by 10 publications

(8 citation statements)

References 23 publications

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“…The TTC denotes the presence of active dehydrogenase enzymes (Beyhan & Serdar 2008), whereas Lugol's solution denotes the presence of starch (Ge et al 2011;Hasnunnahar et al 2012).…”

Section: Methodsmentioning

confidence: 99%

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

et al. 2013

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The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO 3 ).4H 2 O (0.03%), Mg(SO 4 ).7H 2 O (0.02%), KNO 3 (0.01%), H 3 BO 3 (0.01%), sucrose (15%) and agar (0.8%). Although P. edulis and P. setacea showed the same shape and type of pollen aperture, the two differed in terms of their morphology and exine ornamentation pattern. In vitro analysis showed that one of the P. edulis f. flavicarpa accessions (designated BGP 330) presented the highest germination rate (53.98%) and longest pollen tube (2.18 mm). The histochemical analysis overestimated pollen viability when compared with the in vitro results. The results of this study contribute to the breeding of Passiflora species by increasing the understanding of their morphology and pollen grain viability.

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“…The TTC denotes the presence of active dehydrogenase enzymes (Beyhan & Serdar 2008), whereas Lugol's solution denotes the presence of starch (Ge et al 2011;Hasnunnahar et al 2012).…”

Section: Methodsmentioning

confidence: 99%

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

et al. 2013

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“…These dyes were used to identify the most efficient to reveal the pollen viability of passion fruit. The TTC indicates the presence of active dehydrogenase enzymes (BEYHAN and SERDAR, 2008), and the Lugol's solution indicates the presence of starch (HASNUNNAHAR et al, 2012).…”

Section: Pollen Grain Histochemical Analysismentioning

confidence: 99%

In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

et al. 2013

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-The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO 3 ) 4H 2 O, 0.02% of Mg(SO 4 ).7H 2 O, 0.01% of KNO 3 , 0,01% of H 3 BO 3 , 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro. Index terms: passion fruit, pollen germination, histochemical analysis, wild passion fruit. GERMINAÇÃO DE PÓLEN IN VITRO E VIABILIDADE POLÍNICA EM MARACUJAZEIRO (Passiflora spp.)RESUMO -O uso de Passifloras como plantas ornamentais é recente, e por isso pouco se sabe sobre a possibilidade de cruzamento entre diferentes espécies, bem como sobre a viabilidade polínica. O objetivo do trabalho foi avaliar a viabilidade do pólen de seis espécies de maracujazeiro coletados em diferentes estádios de desenvolvimento fisiológico por meio da germinação in vitro, bem como via análise histoquímica, por meio do uso de corantes. O pólen foi coletado em três estádios (pré-antese, antese e pós-antese). Foram comparadas três composições de meios de cultura para avaliar a germinação in vitro, e para a análise histoquímica utilizou-se de dois corantes o 2,3,5-cloreto de trifeniltetrazólio (TTC) e o lugol. O meio de cultura contendo 0,03% de Ca(NO3).4H2O, 0,02% de Mg(SO4).7H2O, 0,01% de KNO3, 0,01% de H3BO3, 15% de sacarose, solidificado com 0,8% de ágar e pH ajustado para 7,0, proporcionou maior percentual de grãos de pólen germinados. O melhor momento de coleta do pólen com relação à melhor viabilidade e à germinação é na antese. O corante TTC e o lugol superestimaram a viabilidade de pólen, já que todos os acessos apresentaram índice de viabilidade alto, quando comparados aos resultados obtidos in vitro. Termos para Indexação: Maracujazeiro, germinação de pólen, análise histoquímica, espécies silvestres.

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“…Pollen and seed fertility characteristics were investigated in the anther indehiscent male sterility systems of eggplant developed by utilizing the cytoplasms of S. kurzii, S. violaceum, and S. virginianum to find out the possibilities of propagation by selfing and the production of pure lines through anther culture (Hasnunnahar et al, 2012b). Pollen fertility was assessed at 2 days before anthesis, on the day of anthesis and 2 days after anthesis by pollen stainability in acetocarmine and in Lugol's solution, and the in vitro germination rate of pollen (Figs.…”

Section: ) Characterization Of Anther Indehiscent Male Sterility Sysmentioning

confidence: 99%

Cytoplasmic Male Sterility in Eggplant

Khan

Isshiki

2016

The Hortic J

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Cytoplasmic male sterility (CMS) is a useful system to produce hybrid seeds in a variety of crop species. In eggplant, CMS systems were developed utilizing the cytoplasms of six wild Solanum species by repeated backcrossings. These CMS systems were classified into two types. The first one is anther indehiscent-type sterility in the CMS systems of eggplant with the cytoplasms of Solanum kurzii Brace & Prain, S. violaceum Ort., and S. virginianum L., in which anther contains normal pollen but does not open to release. The second one is pollen non-formation-type sterility in the CMS systems of eggplant with the cytoplasms of S. aethiopicum Aculeatum Group, S. anguivi Lam., and S. grandifolium C.V. Morton, in which anther of the male-sterile lines is completely devoid of pollen. Both types of sterility system were characterized by investigating pollen and seed fertility. Furthermore, in pollen non-formation CMS systems, two independent dominant fertility restorer (Rf) genes were discovered and a sequenced characterized amplified region (SCAR) marker tightly linked to these genes was developed.

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Pollen and seed fertility of three functional male-sterile lines of eggplant with the wild Solanum cytoplasms

Cited by 10 publications

References 23 publications

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)

In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

Cytoplasmic Male Sterility in Eggplant

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