Pollen‐mediated flow of herbicide resistance genes in Beckmannia syzigachne

Abstract

BACKGROUND: Beckmannia syzigachne (Steud.) Fernald has evolved herbicide resistance due to the long-term sole use of herbicides and has become a dominant weed in wheat fields in the middle and lower reaches of the Yangtze River in China. In addition to the selection pressure imposed by herbicides, pollen-mediated gene flow (PMGF) has been reported to cause the spread of herbicide resistance between populations within a certain range in some farmland weeds. It is not clear whether the same is true for the self-pollinated grass weed B. syzigachne. RESULTS:In this study, we confirmed and quantified the level of PMGF in B. syzigachne through concentric circle planting and herbicide resistance tests. Results show that when the B. syzigachne pollen donor was close to the recipient (0.5 m), the average gene flow was 0.66%. Gene flow was detected as far as 10 m (the farthest distance studied) and decreased exponentially with increasing distance, which could be described by a double exponential decay model. Temperature also affected gene flow, whilst the average level of gene flow in all directions of wind was similar and wind speed caused insignificant difference in gene flow. CONCLUSION:The results of this study confirmed that PMGF can occur between B. syzigachne populations in adjacent fields. Although the level of resistance spreading by pollen was low, especially across long distance, the results were relevant for smallholding farms, which is the dominant form of agricultural operation in China. It is therefore important to take proactive measures and integrate chemical and ecological weed control methods to prevent the spread of resistant B. syzigachne via both seeds and pollens.