Pollen Micromorphology among Amaranthaceous Species from Desert Rangeland: Exine Stratification and their Taxonomic Significance

Abstract

The aim of the study was to visualize the micromorphology of Amaranthaceous pollen using scanning electron microscopy collected from the Thal Desert. Field collection was conducted from July to September 2021. A total of 14 taxa of the family Amaranthaceae were collected which belong to nine genera. Achyranthes aspera, Aerva javanica, Aerva lanata, Amaranthus graecizans, Amaranthus retroflexus, Amaranthus viridis, Bassia indica, Chenopodium album, Chenopodium ficifolium, Chenopodium murale, Digera muricata, Haloxylon stocksii, Salsola tragus, and Suaeda fruticosa were studied in terms of pollen morphotypes. Pollen were acetolyzed and observed under optical and scanning microscopy. Qualitative and quantitative characters were measured to analyze the pollen to uncover its taxonomic significance. Qualitative characters observed were the shape of pollen in polar and equatorial views; the most frequent shape observed was spheroidal in the polar view, whereas in the equatorial view, prolate spheroidal was the dominant shape. Exine ornamentation is the key characteristic of pollen which is very helpful, and eight different types of ornamentations were observed in collected taxa: smooth sparsely granulate, scabrate-spinulose, microspinulose perforate, microechinate scabrate to metareticulate, granulate, nanospinulate, granulate-spinulose perforate, granulate-perforate echinate, and microechinate perforate. Periporate-type aperture was observed among all taxa. Mesoporia, ektexinous bodies, and tectum features also show variations among Amaranthaceous grains. In quantitative character, A. retroflexus recorded highest in polar view 26.3 μm and the lowest was of C. album 12.2 μm. Highest P/E index ratio was recorded in S. fruticosa (1.12) whereas the lowest for D. muricta (0.94). Exine thickness was highest in S. tragus 2.15 μm and lowest in A. graecizans 0.78 μm. The maximum number of pores was recorded as 32-36 in D. muricata. Artificial taxonomic keys were constructed based on findings that reinforce the importance of the micromorphological ultrastructural diversity of pollen among Amaranthaceous taxa. It was concluded that the descriptions of pollen morphotypes presented greatly contribute to our understanding of desert species identification.

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Palyno‐taxonomic approach through light and scanning electron microscopy for the identification of herbaceous Asteraceae species from Sikaram Mountain Pak‐Afghan border

“…Microcharacters including palynological and anatomical attributes were significantly considered in the identification of problematic taxa with their inter‐relationship with other taxa (Abbas et al, 2022; Çitak et al, 2021; Majeed, Ahmad, Ozdemir, et al, 2023), whereas they also assess in the classification of morphologically closely related taxa at tribe, genera, sections, and subspecies levels (Majeed et al, 2022; Shaheen et al, 2018; Via do Pico et al, 2016). Recently, pollen features were applied as phylogenetic traits in the identification and classification of phenotypically similar taxa within the groups (Majeed, Ahmad, Ali, et al, 2023; Younis et al, 2021). Further, based on pollen characters taxonomists were arrange plant taxa in their corresponding correct taxonomic rank.…”