Pollen morphology and in vitro germination characteristics of nodulating and nonnodulating soybean (Glycine max L.) genotypes

Et, Gwata; Ds, Wofford; Pl, Pfahler; Kj, Boote

doi:10.1007/s00122-002-1097-8

Cited by 26 publications

(22 citation statements)

References 18 publications

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“…Studies have indicated differences in the in vitro pollen grain germination as a result of the complex interaction between morphology and physiology of the pollen grain and components of the medium (Gwata et al 2003). Kakani et al (2005) claimed that the differences observed in the germination in vitro and pollen tube growth of 12 cotton cultivars (Gossypium hirsutum) were a reflex of the variability in the cultivars.…”

Section: Resultsmentioning

confidence: 99%

In vitro germination and viability of pollen grains of banana diploids

Soares¹,

Silva²,

Costa³

et al. 2008

CBAB

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Section: Resultsmentioning

confidence: 99%

In vitro germination and viability of pollen grains of banana diploids

Soares¹,

Silva²,

Costa³

et al. 2008

CBAB

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“…Three open flowers were collected 3 days per week for 3 week. The percentage of germinated grains was determined after 18-h incubation in the medium described by Gwata et al (2003) The meiotic abnormalities and meiotic products observed for the BC 4 F 2:3 Rps8/rps8 heterozygous lines were also identified on the anthers of BC 4 F 2:3 Rps8/rps8 heterozygous plants that were grown in a preliminary study, during the summer of 2008 (JuneAugust). The plants evaluated on the preliminary study included four lines from the same BC 4 F 2:3 population evaluated on this study, including RIL 1412, and six lines from another BC 4 F 2:3 .…”

Section: Cytogeneticsmentioning

confidence: 99%

“…Three flowers were collected from each plant, three times a week. Each set of anthers was dissected and dusted onto pollen germination medium (Gwata et al 2003) and incubated at 27°C for 18 h. A minimum of 100 pollen grains per anther were observed for germination under a S6D Stereozoom microscope (Leica Microsystems Inc., Deerfield, Illinois, USA). A grain was classified as germinated if a recognizable pollen tube, at least 20 lm long was present.…”

Section: Pollen Viability and Germinationmentioning

confidence: 99%

Microsporogenesis of Rps8/rps8 heterozygous soybean lines

Ortega

Dorrance

2011

Euphytica

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Phytophthora root and stem rot caused by Phytophthora sojae, is one of the most damaging diseases of soybean, for which management is principally done by planting resistant cultivars with race specific resistance which are conferred by Rps (Resistance to Phytophthora sojae) genes. The Rps8 locus, identified in the South Korean landrace PI 399073, is located in a 2.23 Mbp region on soybean chromosome 13. In eight cv. Williams (rps8/ rps8) 9 PI 399073 (Rps8/Rps8) populations, this region exhibited strong segregation distortion. In a cross between the South Korean lines PI 399073 (Rps8/Rps8) and PI 408211B (multiple Rps genes) this region segregated in a Mendelian fashion. In this study, microsporogenesis was evaluated to identify meiotic abnormalities that may be associated with the segregation distortion of the Rps8 region. Pollen was collected from greenhouse-grown plants of the parental genotypes: Williams, PI 399073, and PI 408211B; as well as selected Rps8/rps8 RILs from Williams 9 PI 399073 BC 4 F 2:3 and PI 399073 9 PI 408211B F 4:5 populations. There were no differences for pollen viability among the genotypes. However, for PI 399073, a mix of dyads, triads, tetrads and pentads was observed. A high frequency of meiotic abnormalities including fragments, laggards, multinucleated microspores; and microcytes containing DNA was also observed in Rps8/ rps8 Williams 9 PI 399073 BC 4 F 2:3 RILs. These meiotic abnormalities may contribute to the high degree of segregation distortion present in the Williams 9 PI 399073 populations.

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“…Non-nodulating soybean mutants have been constructed by chemical mutagenesis and cannot be infected by rhizobia 10,11,46) . In field-grown soybeans, clostridia were detected only in the non-nodulating mutant by TRFLP analysis (Tables 1 and 2).…”

Section: Effect Of Soybean Nodulation Genotypes On Bacterial Communitymentioning

confidence: 99%

Broad Distribution and Phylogeny of Anaerobic Endophytes of Cluster XIVa Clostridia in Plant Species Including Crops

et al. 2008

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Endophytic clostridia present on various plants as obligate anaerobes were surveyed by terminal restriction fragment length polymorphism (TRFLP) analysis specific to the clostridial 16S rRNA gene. Endophytic clostridia were detected in 10 plant types: sugarcane, cultivated rice, corn, tobacco, soybean, bermuda grass, tall fescue, and three mangrove species. Phylogenetically, cluster XIVa clostridia were detected more frequently than cluster I clostridia in aerial parts. Isolation of clostridia from surface-sterilized sugarcane stem validated the TRFLP results. Plant-derived clostridia occupied two unique phylogenetic positions (groups I and II) within cluster XIVa. Most of cluster XIVa clostridia from other sources (e.g., human, animal, and insect intestines) were located outside these groups. Thus two unique groups of cluster XIVa clostridia are widely distributed in plants, including crops. In field-grown soybeans, TRFLP analysis revealed clostridia only in a non-nodulating mutant. Ribosomal intergenic spacer analysis (RISA) showed that the bacterial community in soybean shoot depended partly on the soybean nodulation genotype.Key words: Clostridium, cluster XIVa, endophyte, sugarcane, soybean Endophytes are microorganisms that can colonize living plant tissues without causing any apparent damage to the host 3,23,24,35) . Several diazotrophs have been isolated and characterized as nitrogen-fixing endophytes in plants, including Gluconacetobacter diazotrophicus 3,22) , Herbaspirillum spp. 9,33,38,48) , and Azoarcus sp.19,31) . Novel anaerobic endophytic clostridia and their anaerobic nitrogen-fixing consortium (ANFICO) were recently isolated and characterized in grasses during attempt to overcome a problem with culturing nitrogen-fixing microbes 26) . ANFICO consists of nitrogenfixing clostridia and diverse non-diazotrophic bacteria and expresses nitrogen-fixing activity associated with low oxygen tension and interspecies inducers 26) . Phylogenetic analysis indicated that the endophytic clostridia fell exclusively into clusters XIVa and I, as defined by Collins et al. 8) , and were further subdivided into five groups 26) . Miyamoto et al.27) developed a terminal restriction fragment length polymorphism (TRFLP) detection system specific for these clusters and groups of plant clostridia, and demonstrated that the group II clostridia in cluster XIVa 8)dominated the populations of diazotrophs and clostridia in a gramineous grass, Miscanthus sinensis. Inoculation examinations indicate that endophytic clostridia colonize plant tissues, alleviating the damage caused by salt stress 47) and potentially fixing nitrogen in the plant 34) . Thus, more attention should be paid to the endophytic clostridia in terms of their distribution, phylogeny, and function.Recently, cluster XIVa clostridia have been detected and isolated from human faeces 12,18,28) , animal intestines 2,20,21,25,44) , termite guts 40,41) , soil 7,17,45) , and plants 26,27) using culture-independent and culture-dependent methods. However, the relations...

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Pollen morphology and in vitro germination characteristics of nodulating and nonnodulating soybean (Glycine max L.) genotypes

Cited by 26 publications

References 18 publications

In vitro germination and viability of pollen grains of banana diploids

In vitro germination and viability of pollen grains of banana diploids

Microsporogenesis of Rps8/rps8 heterozygous soybean lines

Broad Distribution and Phylogeny of Anaerobic Endophytes of Cluster XIVa Clostridia in Plant Species Including Crops

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