Pollen morphology and its systematic implication on some species of <i>Artemisia</i> L. from Gilgit-Baltistan Pakistan

Abstract

This study was accomplished to scrutinize the pollen morphology of 15 species of the genus Artemisia of the family Asteraceae from Gilgit-Baltistan region of Pakistan by means of scanning electron microscopy (SEM). Results revealed pollen grains of Artemisia species with tricolporate shape, and characterized by globular symmetry (ellipsoid ball shaped from equatorial side and three lobed rounds from polar view) with few exceptions. Additionally, the pollens are marked with reduced spinules on their surfaces which are diagnostic character for the genus Artemisia. In this study, seven micromorphological characters of pollen grains of 15 Artemisia species viz. shape of pollen, arrangement of spinules, exine sculpture, spinules base, equatorial width and polar length, were employed to construct a dendrogram following the consequential cluster analyses. In the dentrogram, four groups within the studied Artemisia species have been recognized. The pollen morphology of Artemisia could be a good taxonomic marker to cope with its taxonomic delimitations in combination with other floral and molecular attributes.

Palyno‐morphological diversity of Asteraceous and Poaceous allergenic plant using microscopic techniques in lesser Himalaya‐Pakistan

“…Anther were squashed and then acetolyzed pollen grains were suspended in 90% ethanol and then mounted on prepared stubs. These samples were sputter coated with gold palladium and observed under Scanning electron microscope JEOL JSM5910 Model (Butt et al, 2018; Hussain et al, 2019). Pollen exine ornamentation was studied following the terminology of Bahadur et al (2018).…”

Comparative palynology and taxonomic implication of Jasminum L. (Oleaceae) species from Pakistan on the bases of scanning electron microscopy

“…The pollen material used was obtained from herbarium specimens and prepared for SEM analysis using sticky carbon disc on metal stub and coated with platinum in a sputtering chamber (Pelco Auto sputter Coater SC‐7, Ted Pella Inc). Philips XL30 TMP (FEI Company) electron microscope was used to analyze the samples at 20 kV (Hussain, Hayat, Sahreen, & Bokhari, 2019; Hussain, Potter, Hayat, Sahreen, & Bokhari, 2019), at the core electron microscopy laboratory, Tupper Hall, University of California Davis California, USA.…”

Artemisia pollen dataset for exploring the potential ecological indicators in deep time

“…Furthermore, Sing and Joshi (1969) questioned the feasibility of recognizing pollen types under LM in the highly uniform pollen of Artemisia. Later, SEM made it possible to subdivide the pollen of Artemisia and those of other related genera within the Artemisia pollen type using pollen exine ultrastructure characters (Chen, 1987;Chen and Zhang, 1991;Sun and Xu, 1997;Jiang et al, 2005;Ghahraman et al, 2007;Shan et al, 2007;Hayat et al, 2009;Hayat et al, 2010;Hussain et al, 2019).…”

“…For instance, following the description and statistics of pollen morphology of 27 species of Artemisia in Eurasia under LM, Sing and Joshi (1969) stated that the pollen grains of Artemisia are consistent and continuous in morphology. Later, some authors recognized a series of pollen types (Chen, 1987;Jiang et al, 2005;Ghahraman et al, 2007;Shan et al, 2007;Hayat et al, 2009;Hayat et al, 2010;Hussain et al, 2019), based on a detailed survey of the pollen micromorphology of different taxa under the scanning electron microscope (SEM).…”

“…CC BY 4.0 License.of Artemisia. In the latter work, eight micromorphological characters were identified and pooled by cluster analysis, leading to the recognition of 5 groups Hussain et al (2019). studied the pollen morphology of 15 Artemisia species in the Gilgit-Baltistan region of Pakistan utilizing SEM and divided these species into four groups based on cluster analysis of seven micromorphological characters (pollen type, pollen shape, spinule arrangement, exine sculpture, spinule base, polar length, and equatorial width).…”