Pollutant sensing layer based on cytochrome P450

Paternolli, Cristina; Ghisellini, Paola; Nicolini, Claudio

doi:10.1016/s0928-4931(02)00164-9

Cited by 17 publications

(12 citation statements)

References 10 publications

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“…It is increasingly being recognized that these studies have played a significant role in developing various biomolecular devices [2][3][4][5][6][7][8][9][10]. Among the several techniques of producing organized molecular assemblies such as self-assembly, sol-gel process, Langmuir-Blodgett (LB) techniques, etc., LB method is one of the most versatile and convenient techniques for designing ultra thin artificial films with biological functions [11].…”

Section: Introductionmentioning

confidence: 99%

Incorporation of ovalbumin within cationic octadecylamine monolayer and a comparative study with zwitterionic DPPC and anionic stearic acid monolayer

Kamilya

Pal

Talapatra

2007

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Incorporation of ovalbumin within cationic octadecylamine monolayer and a comparative study with zwitterionic DPPC and anionic stearic acid monolayer

Kamilya

Pal

Talapatra

2007

Journal of Colloid and Interface Science

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“…Fusion proteins have found applications in a wide spectrum of areas such as the biomedical field [including immunology, cancer research, and drug delivery (17)(18)(19)(20)] and materials science [self-assembled materials (e.g., gels), quantum dot bioconjugates, sensors, and inorganic materials synthesis (21)(22)(23)(24)(25)(26)(27)]. Here we describe new silica-based nanocomposites formed from bioengineered fusion proteins that consist of two components (Fig.…”

mentioning

confidence: 99%

Novel nanocomposites from spider silk–silica fusion (chimeric) proteins

Foo

Patwardhan

Belton

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

188

147

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Silica skeletal architectures in diatoms are characterized by remarkable morphological and nanostructural details. Silk proteins from spiders and silkworms form strong and intricate self-assembling fibrous biomaterials in nature. We combined the features of silk with biosilica through the design, synthesis, and characterization of a novel family of chimeric proteins for subsequent use in model materials forming reactions. The domains from the major ampullate spidroin 1 (MaSp1) protein of Nephila clavipes spider dragline silk provide control over structural and morphological details because it can be self-assembled through diverse processing methods including film casting and fiber electrospinning. Biosilica nanostructures in diatoms are formed in aqueous ambient conditions at neutral pH and low temperatures. The R5 peptide derived from the silaffin protein of Cylindrotheca fusiformis induces and regulates silica precipitation in the chimeric protein designs under similar ambient conditions. Whereas mineralization reactions performed in the presence of R5 peptide alone form silica particles with a size distribution of 0.5-10 m in diameter, reactions performed in the presence of the new fusion proteins generate nanocomposite materials containing silica particles with a narrower size distribution of 0.5-2 m in diameter. Furthermore, we demonstrate that composite morphology and structure could be regulated by controlling processing conditions to produce films and fibers. These results suggest that the chimeric protein provides new options for processing and control over silica particle sizes, important benefits for biomedical and specialty materials, particularly in light of the all aqueous processing and the nanocomposite features of these new materials.biomaterials ͉ nanostructures ͉ silaffin ͉ biomineralization ͉ ceramics C omplex mineralized composite systems in nature provide rich ground for insight into mechanisms of biomineralization and novel materials designs (1-4). Some of the more common sources of inspiration include seashells, insect exoskeletons, extracellular matrices involved in bone and other hard tissues, and biosilica skeletons. The formation of natural inorganic-organic composites is a multistep process, including the assembly of the extracellular matrix, the selective transportation of inorganic ions to discrete organized compartments with subsequent mineral nucleation, and growth delineated by preorganized cellular compartments. Silica skeletons found in nature are based on nanoscale composites wherein the organic components, usually proteins, are functional parts of the skeletal structures while also serving as silica-forming components (5, 6). As a result, materials' toughness is improved, strength is retained, and fine morphological control is achieved, all hallmark attributes of biological composites.Silica is widespread in biological systems and serves different functions, including support and protection in single-celled organisms, such as diatoms through to higher plants and animals (7,8...

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“…In particular, protein-silica composite materials are explored for application in biomedical fields such as immunology, cancer research, and drug delivery [7,8,9,10]. These hybrid materials are also investigated in materials science technologies such as self-assembled materials, quantum dot bioconjugates, sensors, and inorganic materials synthesis [11,12,13,14,15,16,17].…”

Section: Introductionmentioning

confidence: 99%

Microwave Assisted Sol-Gel Synthesis of Silica-Spider Silk Composites

Giasuddin

Britt

2019

Molecules

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This study introduces a simple and environmentally friendly method to synthesize silica-protein nanocomposite materials using microwave energy to solubilize hydrophobic protein in an aqueous solution of pre-hydrolyzed organo- or fluoro-silane. Sol-gel functionality can be enhanced through biomacromolecule incorporation to tune mechanical properties, surface energy, and biocompatibility. Here, synthetic spider silk protein and organo- and fluoro-silane precursors were dissolved and mixed in weakly acidic aqueous solution using microwave technology. Scanning electron microscopy (SEM) and Atomic force microscopy (AFM) images revealed the formation of spherical nanoparticles with sizes ranging from 100 to 500 nm depending, in part, on silane fluoro- or organo-side chain chemistry. The silane-protein interaction in the nanocomposite was assessed through infrared spectroscopy. Deconvoluted ATR-FTIR (Attenuated total reflectance Fourier-transform infrared spectroscopy) spectra revealed silane chemistry-specific conformational changes in the protein-silane nanocomposites. Relative to microwave-solubilized spider silk protein, the β structure content increased by 14% in the spider silk-organo-silica nanocomposites, but decreased by a net 20% in the spider silk-fluoro-silica nanocomposites. Methods of tuning the secondary structures, and in particular β-sheets that are the cross-linking moieties in spider silks and other self-assembling fibrillar proteins, may provide a unique means to promote protein interactions, favor subsequent epitaxial growth process, and enhance the properties of the protein-silane nanocomposites.

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Pollutant sensing layer based on cytochrome P450

Cited by 17 publications

References 10 publications

Incorporation of ovalbumin within cationic octadecylamine monolayer and a comparative study with zwitterionic DPPC and anionic stearic acid monolayer

Incorporation of ovalbumin within cationic octadecylamine monolayer and a comparative study with zwitterionic DPPC and anionic stearic acid monolayer

Novel nanocomposites from spider silk–silica fusion (chimeric) proteins

Microwave Assisted Sol-Gel Synthesis of Silica-Spider Silk Composites

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