Poly(3,4-ethylenedioxythiophene) (PEDOT) Nanobiointerfaces: Thin, Ultrasmooth, and Functionalized PEDOT Films with in Vitro and in Vivo Biocompatibility

Luo, Shyh‐Chyang; Ali, Emril Mohamed; Tansil, Natalia C.; Yu, Hsiao‐hua; Gao, Shujun; Kantchev, Eric Assen B.; Ying, Jackie Y.

doi:10.1021/la800333g

Cited by 303 publications

(217 citation statements)

References 61 publications

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“…EDOT-OH was purchased from a custom-synthesis company, where it had been prepared following procedures previously reported 66,67 . EDOT-COOH was synthesized following procedures previously reported 68,69 . 2-Maleimidoethylamine hydrochloride and 1-ethyl-3-(3-dimethyaminopropyl)-carbodiimide hydrochloride were purchased from Astatech and Tokyo Chemical Industry, respectively.…”

Section: Methodsmentioning

confidence: 99%

Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer

et al. 2014

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Although electrically stimulated neurite outgrowth on bioelectronic devices is a promising means of nerve regeneration, immunogenic scar formation can insulate electrodes from targeted cells and tissues, thereby reducing the lifetime of the device. Ideally, an electrode material capable of electrically interfacing with neurons selectively and efficiently would be integrated without being recognized by the immune system and minimize its response. Here we develop a cell membrane-mimicking conducting polymer possessing several attractive features. This polymer displays high resistance towards nonspecific enzyme/cell binding and recognizes targeted cells specifically to allow intimate electrical communication over long periods of time. Its low electrical impedance relays electrical signals efficiently. This material is capable to integrate biochemical and electrical stimulation to promote neural cellular behaviour. Neurite outgrowth is enhanced greatly on this new conducting polymer; in addition, electrically stimulated secretion of proteins from primary Schwann cells can also occur on it.

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Section: Methodsmentioning

confidence: 99%

Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer

et al. 2014

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“…Valle et al (2007) investigated the interaction of PEDOT films with epithelial cells and showed the biocompatibility of PEDOT with epithelial cells. Luo et al (2008) synthesized PEDOT films and investigated their biocompatibility by seeding NIH3T3 fibroblasts on PEDOT films, and carried out subcutaneous implantation of PEDOT films by in vivo study. Their results showed that PEDOT films exhibit very low intrinsic cytotoxicity and that their inflammatory response upon implantation was good, making them ideal for biosensing and bioengineering applications.…”

Section: Poly (3 4-ethylenedioxythiophene)mentioning

confidence: 99%

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

Ghasemi‐Mobarakeh¹,

Prabhakaran²,

Morshed³

et al. 2011

J Tissue Eng Regen Med

613

389

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Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε-caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed.

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“…Poly(3,4-ethylenedioxythiophene) (PEDOT) is a biocompatible CP which is recently being employed in biomedical applications, 22 especially in nerve tissue engineering. 23 To gain a water soluble polyelectrolyte system with good film-forming properties, PEDOT is doped with poly(4-styrenesulfonate) (PSS).…”

Section: Introductionmentioning

confidence: 99%

3D conductive nanocomposite scaffold for bone tissue engineering

Shahini¹,

Yazdimamaghani²,

Walker³

et al. 2013

IJN

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Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli.

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Poly(3,4-ethylenedioxythiophene) (PEDOT) Nanobiointerfaces: Thin, Ultrasmooth, and Functionalized PEDOT Films with in Vitro and in Vivo Biocompatibility

Cited by 303 publications

References 61 publications

Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer

Large enhancement in neurite outgrowth on a cell membrane-mimicking conducting polymer

Application of conductive polymers, scaffolds and electrical stimulation for nerve tissue engineering

3D conductive nanocomposite scaffold for bone tissue engineering

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