Poly(amido amine)-Based Mannose-Glycodendrimers As Multielectron Redox Probes for Improving Lectin Sensing

Martos-Maldonado, Manuel C.; Casas‐Solvas, Juan M.; Quesada-Soriano, Indalecio; Garcı́a-Fuentes, Luis; Vargas‐Berenguel, Antonio

doi:10.1021/la304107a

Cited by 20 publications

(24 citation statements)

References 40 publications

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“…They used avidin as a linker for immobilizing biotinylated alkaline phosphatase on the surface of biotin-terminated Fc monolayer films [81]. They studied the voltammetric properties of Fc-sugar and Fc-glycogen conjugates in the presence and absence of lectin [82,83,84,85]. The Fc conjugates will be used to construct sugar sensors.…”

Section: Fc-containing Thin Filmsmentioning

confidence: 99%

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Takahashi

Anzai

2013

Materials

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This article reviews recent progress in the development of ferrocene (Fc)-modified thin films and nanoparticles in relation to their biosensor applications. Redox-active materials in enzyme biosensors commonly use Fc derivatives, which mediate electron transfer between the electrode and enzyme active site. Either voltammetric or amperometric signals originating from redox reactions of Fc are detected or modulated by the binding of analytes on the electrode. Fc-modified thin films have been prepared by a variety of protocols, including in situ polymerization, layer-by-layer (LbL) deposition, host-guest complexation and molecular recognitions. In situ polymerization provides a facile way to form Fc thin films, because the Fc polymers are directly deposited onto the electrode surface. LbL deposition, which can modulate the film thickness and Fc content, is suitable for preparing well-organized thin films. Other techniques, such as host-guest complexation and protein-based molecular recognition, are useful for preparing Fc thin films. Fc-modified Au nanoparticles have been widely used as redox-active materials to fabricate electrochemical biosensors. Fc derivatives are often attached to Au nanoparticles through a thiol-Au linkage. Nanoparticles consisting of inorganic porous materials, such as zeolites and iron oxide, and nanoparticle-based composite materials have also been used to prepare Fc-modified nanoparticles. To construct biosensors, Fc-modified nanoparticles are immobilized on the electrode surface together with enzymes.

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Section: Fc-containing Thin Filmsmentioning

confidence: 99%

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Takahashi

Anzai

2013

Materials

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“…The same group reported applying these modified electrodes with gold nanoparticles modified with either Con A or CramoLL lectin to the detection of ovalbumin [46, 47]. In a recent study, the binding of Con A to PAMAM dendrimers modified by mannopyranosyl ferrocene units was detected as a reduction in the peak current due to ferrocene oxidation using DPV [48]. The same group also developed mannopyranosyl ferrocene modified gold nanoparticles for DPV detection of Con A binding [49].…”

Section: Introductionmentioning

confidence: 99%

Square-wave voltammetry assays for glycoproteins on nanoporous gold

Pandey

Bhattarai

Pornsuriyasak

et al. 2014

Journal of Electroanalytical Chemistry

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Electrochemical enzyme-linked lectinsorbent assays (ELLA) were developed using nanoporous gold (NPG) as a solid support for protein immobilization and as an electrode for the electrochemical determination of the product of the reaction between alkaline phosphatase (ALP) and p-aminophenyl phosphate (p-APP), which is p-aminophenol (p-AP). Glycoproteins or concanavalin A (Con A) and ALP conjugates were covalently immobilized onto lipoic acid self-assembled monolayers on NPG. The binding of Con A – ALP (or soybean agglutinin – ALP) conjugate to glycoproteins covalently immobilized on NPG and subsequent incubation with p-APP substrate was found to result in square-wave voltammograms whose peak difference current varied with the identity of the glycoprotein. NPG presenting covalently bound glycoproteins was used as the basis for a competitive electrochemical assay for glycoproteins in solution (transferrin and IgG). A kinetic ELLA based on steric hindrance of the enzyme-substrate reaction and hence reduced enzymatic reaction rate after glycoprotein binding is demonstrated using immobilized Con A–ALP conjugates. Using the immobilized Con A-ALP conjugate, the binding affinity of immunoglobulin G (IgG) was found to be 105 nM, and that for transferrin was found to be 650 nM. Minimal interference was observed in the presence of 5 mg mL−1 BSA as a model serum protein in both the kinetic and competitive ELLA. Inhibition studies were performed with methyl D-mannoside for the binding of TSF and IgG to Con A-ALP; IC50 values were found to be 90 μM and 286 μM, respectively. Surface coverages of proteins were estimated using solution depletion and the BCA protein concentration assay.

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“…(b) Differential pulse voltammetry showing decrease in the peak current upon binding of Man-dendrimer with ConA. Adapted from (Martos-Maldonado et al 2013) with permission from American Chemical Society.…”

Section: Figmentioning

confidence: 99%

Glyconanomaterials for biosensing applications

Hao

Neranon

Ramström

et al. 2016

Biosensors and Bioelectronics

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Nanomaterials constitute a class of structures that have unique physiochemical properties and are excellent scaffolds for presenting carbohydrates, important biomolecules that mediate a wide variety of important biological events. The fabrication of carbohydrate-presenting nanomaterials, glyconanomaterials, is of high interest and utility, combining the features of nanoscale objects with biomolecular recognition. The structures can also produce strong multivalent effects, where the nanomaterial scaffold greatly enhances the relatively weak affinities of single carbohydrate ligands to the corresponding receptors, and effectively amplifies the carbohydrate-mediated interactions. Glyconanomaterials are thus an appealing platform for biosensing applications. In this review, we discuss the chemistry for conjugation of carbohydrates to nanomaterials, summarize strategies, and tabulate examples of applying glyconanomaterials in in vitro and in vivo sensing applications of proteins, microbes, and cells. The limitations and future perspectives of these emerging glyconanomaterials sensing systems are furthermore discussed.

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Poly(amido amine)-Based Mannose-Glycodendrimers As Multielectron Redox Probes for Improving Lectin Sensing

Cited by 20 publications

References 40 publications

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Square-wave voltammetry assays for glycoproteins on nanoporous gold

Glyconanomaterials for biosensing applications

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