Poly(amidoamine) dendrimer‐coated magnetic nanoparticles for the fast purification and selective enrichment of glycopeptides and glycans

Abstract

Glycosylated proteins modulate various important functions of organisms. To reveal the functions of glycoproteins, in-depth characterization studies are necessary. Although mass spectrometry is a very efficient tool for glycoproteomic and glycomic studies, efficient sample preparation methods are required prior to analyses. In the study, poly(amidoamine) dendrimer-coated magnetic nanoparticles were presented for the specific enrichment and fast purification of glycopeptides and glycans. The enrichment and purification performance of the developed method was evaluated both at the glycopeptide, and the glycan level using several standard glycoprotein digests and released glycan samples. The poly(amidoamine) dendrimer-coated magnetic nanoparticles not only showed selective affinity (Immunoglobulin G/Bovine Serum Albumin, 1/10 by weight) to glycopeptides and released glycans but also good sensitivity (0.4 ng/μL for Immunoglobulin G) for glycoproteomic and glycomic applications. Thirty-five glycopeptides of Immunoglobulin G were detected after enrichment with poly(amidoamine) dendrimer-coated magnetic nanoparticles. In addition, 55 18 O tagged deamidated glycopeptides belonging to human plasma glycoproteome were confirmed. Finally, fifty 2-aminobenzoic acid, and 30 procainamide-labelled human plasma N-glycans released from human plasma glycoproteins were determined after purifications. The results indicate that the proposed enrichment and purification method using poly(amidoamine) dendrimer-coated magnetic nanoparticles could be simply adjusted to sample preparation methods.

In situ green synthesis of MnFe2O4@EP@Ag nanocomposites using Epilobium parviflorum green tea extract: An efficient magnetically recyclable catalyst for the reduction of hazardous organic dyes

“…Manganese ferrite (MnFe 2 O 4 ) NPs have been studied extensively in many fields involving medicine, drug delivery, and chemical sensing and have offered good potentials in the field of catalysis depending on its being stable, inexpensive, and magnetically recoverable [38][39][40][41][42][43][44]. Despite this interest, no one to the best of our knowledge has studied MnFe 2 O 4 NPs as a core support material for the coating of a plant or a green tea extract for the immobilization of metal nanoparticles to produce an efficient and recoverable catalyst for their potential applications in the related fields so far.…”

In situ deposition of silver nanoparticles on polydopamine‐coated manganese ferrite nanoparticles: Synthesis, characterization, and application to the degradation of organic dye pollutants as an efficient magnetically recyclable nanocatalyst

“…[33,40,44] Among MgNPs, manganese ferrite (MnFe 2 O 4 ) NPs come to a focal point and used in variety of numerous applications, including chemical sensing, drug delivery, and medicine due to their advantages of being inexpensive, magnetically recoverable, and highly stable. [45][46][47][48] However, no one to the best of our knowledge has studied the MnFe 2 O 4 NPs as a core or support material for the PDA coating to immobilize the AgNPs to develop efficient magnetically recyclable nanocatalysts for the target interest of catalytic applications for wastewater treatment.…”

Extraction and determination of flavonoids in fruit juices and vegetables using Fe3O4/SiO2 magnetic nanoparticles modified with mixed hemi/ad‐micelle cetyltrimethylammonium bromide and high performance liquid chromatography

“…Nowadays, magnetic nanoparticles (MNPs) work as the sorbent for MSPE. Magnetic nanoparticles offer several advantages such as having a short time for extraction, small solvent consumption, low toxicity, low cost, and high extraction recovery .…”