Poly (ethylene glycol) hydrogel scaffolds with multiscale porosity for culture of human adipose-derived stem cells

Barnett, Haley; Heimbuck, Abitha M.; Pursell, India; Hegab, Rachel A.; Sawyer, Benjamin J.; Newman, Jamie J.; Caldorera‐Moore, Mary

doi:10.1080/09205063.2019.1612725

Cited by 10 publications

(4 citation statements)

References 72 publications

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“…A recent study by Parmenter et al (2016) showed the possibility of using cryogenic focused ion beam scanning electron microscopy to visual the morphology while preserving the water volume content in samples, thereby avoiding structure collapse by shrinking, also involving gel plunge freezing in the preparatory process [28]. Another approach avoiding sample freezing is used in environmental scanning electron microscopy (ESEM) [29]. Nevertheless, this method has still some drawbacks compared to SEM-e.g., its lower resolution or decrease in contrast.…”

Section: Introductionmentioning

confidence: 99%

Revealing the True Morphological Structure of Macroporous Soft Hydrogels for Tissue Engineering

Podhorská

Vetrík

Chylíková-Krumbholcová

et al. 2020

Applied Sciences

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(1) Background: Macroporous hydrogel scaffolds based on poly [N-(2-hydroxypropyl) methacrylamide] are one of the widely studied biocompatible materials for tissue reparation and regeneration. This study investigated the morphological changes during hydrogel characterization which can significantly influence their future application. (2) Methods: Three types of macroporous soft hydrogels differing in pore size were prepared. The macroporosity was achieved by the addition of sacrificial template particles of sodium chloride of various sizes (0–30, 30–50, and 50–90 µm) to the polymerizing mixture. The 3D structure of the hydrogels was then investigated by scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). The SEM was performed with specimens rapidly frozen to various temperatures, while non-frozen gels were visualized with LSCM. (3 and 4) Results and Conclusion: In comparison to LSCM, the SEM images revealed a significant alteration in the mean pore size and appearance of newly formed multiple connections between the pores, depending on the freezing conditions. Additionally, after freezing for SEM, the gel matrix between the pores and the fine pores collapsed. LSCM visualization aided the understanding of the dynamics of pore generation using sodium chloride, providing the direct observation of hydrogel scaffolds with the growing cells. Moreover, the reconstructed confocal z-stacks were a promising tool to quantify the swollen hydrogel volume reconstruction which is not possible with SEM.

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Section: Introductionmentioning

confidence: 99%

Revealing the True Morphological Structure of Macroporous Soft Hydrogels for Tissue Engineering

Podhorská

Vetrík

Chylíková-Krumbholcová

et al. 2020

Applied Sciences

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“…Next, we asked whether there would be variation in particle retention within the hydrogels over time. Considering the nanoscale size of the Fe 3 O 4 in contrast to the microscale meshwork of the radical polymerized PEG gels (average pore size ranges between 5 to 70 μm), [ 51,52 ] we expected easy diffusion of the nanoparticles out of the hydrogel. However, when subjected to a magnetic field up to 48 h followed by centrifugation of the media, no evidence of particle release from the hydrogels was found and there was no difference between the surface coatings.…”

Section: Resultsmentioning

confidence: 99%

Magnetic Nanocomposite Hydrogels for Directing Myofibroblast Activity in Adipose‐Derived Stem Cells

Islam

Molley

Ireland

et al. 2021

Advanced NanoBiomed Research

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Dynamic cell‐culture materials that can change mechanical properties in response to extrinsic stimuli are emerging as promising tools for cell and tissue engineering research. However, most of these techniques involve a one‐way stiffening or softening through changes in the materials chemistry, which does not allow reversibility. Here, the incorporation of superparamagnetic iron‐oxide nanoparticles within poly(ethylene glycol) hydrogels as dynamic cell culture materials is demonstrated. Using simple permanent magnets and adipose‐derived stem cells, a near twofold increase in cell spread area and an accompanying 20% enrichment in cells expressing alpha‐smooth muscle actin is seen. This platform provides a means to study relationships between dynamic stiffening and cell behavior, using permanent magnets and clinically viable composite materials, with scope for use as a tool to enrich the myofibroblast population in stromal cells.

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“…Some studies have combined PEG with other polymers for the targeted delivery of angiogenic substances. Additionally, certain PEG derivatives, like poly(ethylene glycol) dimethacrylate (PEGDMA), have garnered interest as intriguing targets. , Poly(ethylene glycol)-coupled polymers enhance angiogenesis while also transporting medications or bioactive molecules to the site of damage, thus reducing the inflammatory response. Since these polymers may pass both the blood–spinal cord and blood–brain barriers, they are commonly utilized as medication carriers.…”

Section: Multifaceted Effects Of Biomaterials Enhance Endothelial Cel...mentioning

confidence: 99%

Unraveling Endothelial Cell Migration: Insights into Fundamental Forces, Inflammation, Biomaterial Applications, and Tissue Regeneration Strategies

Jerka,

Bonowicz,

Piekarska

et al. 2024

ACS Appl. Bio Mater.

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Cell migration is vital for many fundamental biological processes and human pathologies throughout our life. Dynamic molecular changes in the tissue microenvironment determine modifications of cell movement, which can be reflected either individually or collectively. Endothelial cell (EC) migratory adaptation occurs during several events and phenomena, such as endothelial injury, vasculogenesis, and angiogenesis, under both normal and highly inflammatory conditions. Several advantageous processes can be supported by biomaterials. Endothelial cells are used in combination with various types of biomaterials to design scaffolds promoting the formation of mature blood vessels within tissue engineered structures. Appropriate selection, in terms of scaffolding properties, can promote desirable cell behavior to varying degrees. An increasing amount of research could lead to the creation of the perfect biomaterial for regenerative medicine applications. In this review, we summarize the state of knowledge regarding the possible systems by which inflammation may influence endothelial cell migration. We also describe the fundamental forces governing cell motility with a specific focus on ECs. Additionally, we discuss the biomaterials used for EC culture, which serve to enhance the proliferative, proangiogenic, and promigratory potential of cells. Moreover, we introduce the mechanisms of cell movement and highlight the significance of understanding these mechanisms in the context of designing scaffolds that promote tissue regeneration.

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Poly (ethylene glycol) hydrogel scaffolds with multiscale porosity for culture of human adipose-derived stem cells

Cited by 10 publications

References 72 publications

Revealing the True Morphological Structure of Macroporous Soft Hydrogels for Tissue Engineering

Revealing the True Morphological Structure of Macroporous Soft Hydrogels for Tissue Engineering

Magnetic Nanocomposite Hydrogels for Directing Myofibroblast Activity in Adipose‐Derived Stem Cells

Unraveling Endothelial Cell Migration: Insights into Fundamental Forces, Inflammation, Biomaterial Applications, and Tissue Regeneration Strategies

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