Poly(glycidylmethacrylate) brushes generated on poly(VBC) beads by SI-ATRP technique: Hydrazine and amino groups functionalized for invertase adsorption and purification

Materials Science and Engineering: C

Bitirim

Tunalı

et al. 2013

Keywords: Hydrogels biocompatible polymers contact angle blood cell adhesion hemolysis rat mesenchymal stem cells Copolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α′-azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant \NH 2 group were used for modifications of the p(HEMA-GMA) films. The \NH 2 group served as anchor binding site for immobilization of functional groups on the hydrogel film via direct epoxy ring opening reaction. The modified hydrogel films were characterized by FTIR, and contact angle studies. In addition, mechanical properties of the hydrogel films were studied, and modified hydrogel films showed improved mechanical properties compared with the non-modified film, but they are less elastic than the non-modified film. The biological activities of these films such as platelet adhesion, red blood cells hemolysis, and swelling behavior were studied. The effect of modified hydrogel films, including \NH 2 , (using different aliphatic \CH 2 chain lengths) \CH 3 , \SO 3 H, aromatic groups with substituted \OH and \COOH groups, and amino acids were also investigated on the adhesion, morphology and survival of rat mesenchymal stem cells (MSCs). The MTT colorimetric assay reveals that the p(HEMA-GMA)-GA-AB, p(HEMA-GMA)-GA-Phe, p(HEMA-GMA)-GA-Trp, p(HEMA-GMA)-GA-Glu formulations have an excellent biocompatibility to promote the cell adhesion and growth. We anticipate that the fabricated p(HEMA-GMA) based hydrogel films with controllable surface chemistry and good stable swelling ratio may find extensive applications in future development of tissue engineering scaffold materials, and in various biotechnological areas.

Section: Synthesis Modification and Characterization Of The Modifiedmentioning

confidence: 99%

Section: Synthesis Modification and Characterization Of The Modifiedmentioning

confidence: 99%

Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

Materials Science and Engineering: C

Bitirim

Tunalı

et al. 2013

“…The chemical reactions are presented schematically in Figure . The successful use of ATRP in controlled polymerisation of several monomers in aqueous media under mild conditions has recently opened up a new pathway for surface‐initiated grafting of different monomers , and ATRP appeared to be the method mostly used because of its easy manipulation, ability to control the molecular weight and structure of the grafted polymer. The specific surface area of the p(HEMA/EGDMA‐g‐GMA) resin was measured by the BET method and was found to be 17.9 m 2 /g beads.…”

Section: Resultsmentioning

confidence: 99%

Removal of reactive dyes from wastewater by acrylate polymer beads bearing amino groups: isotherm and kinetic studies

Arıca

2013

Coloration Technology

The aim of this study was to prepare a novel resin for the removal of reactive dyes from aqueous media. To prepare the resin, poly(2‐hydroxyethyl methacrylate/ethylene glycol dimethacrylate) beads were grafted with poly(glycidyl methacrylate) by surface‐initiated atom transfer radical polymerisation. Epoxy groups of the grafted polymer were modified with tris(2‐aminoethyl)amine ligand. The modified resin was characterised by swelling studies, FT‐IR and SEM. Three different reactive dyes were selected (CI Reactive Brown 10, CI Reactive Red 120 and CI Reactive Green 5) and used in the removal studies. The effects of pH, temperature, ionic strength and initial dye concentration on the adsorption capacity of the resin were investigated. The adsorption capacity of the resin for Reactive Brown 10, Reactive Red 120 and Reactive Green 5 was 0.029 ± 0.010, 0.032 ± 0.0019 and 0.042 ± 0.0013 mmol/g resin (34.1 ± 1.2, 47.6 ± 2.3 and 69.3 ± 1.7 mg/g resin) respectively. The equilibrium adsorption data were analysed by Langmuir, Dubinin–Radushkevich, Freundlich and Temkin isotherm models. A good fit was found between the Langmuir isotherm and data for the three dyes on resin. The adsorption kinetic data were modelled using pseudo‐first‐order, pseudo‐second‐order and intraparticle diffusion kinetic equations. It was found that the pseudo‐second‐order equation could describe the adsorption kinetics. The results indicated that the modified resin is an attractive alternative for removing reactive dyes from wastewater.

“…However, to avoid the enzyme loss while processing or washing, a relatively strong Van-der Waals interaction between the enzyme and support is essential. Multi-modal ligand functionalized polymers may provide very strong hydrophobic and electrostatic interactions between enzyme and supports to preclude the enzyme leakage [12][13][14][15][16][17][18]. Additional advantage of the reversible immobilization is recovery of the support by easy removal of the inactivated enzyme and it may reuse in further cycles, which makes the process cost effective.…”

Section: Introductionmentioning

confidence: 99%

Poly(styrene–divinylbenzene) beads surface functionalized with di-block polymer grafting and multi-modal ligand attachment: performance of reversibly immobilized lipase in ester synthesis

Karagöz

Altıntaş

et al. 2011

Bioprocess Biosyst Eng

Fibrous poly(styrene-b-glycidylmethacrylate) brushes were grafted on poly(styrene-divinylbenzene) (P(S-DVB)) beads using surface-initiated atom transfer radical polymerization. Tetraethyldiethylenetriamine (TEDETA) ligand was incorporated on P(GMA) block. The ligand attached beads were used for reversible immobilization of lipase. The influences of pH, ionic strength, and initial lipase concentration on the immobilization capacities of the beads have been investigated. Lipase adsorption capacity of the beads was about 78.1 mg/g beads at pH 6.0. The Km value for immobilized lipase was about 2.1-fold higher than that of free enzyme. The thermal, and storage stability of the immobilized lipase also was increased compared to the native lipase. It was observed that the same support enzyme could be repeatedly used for immobilization of lipase after regeneration without significant loss in adsorption capacity or enzyme activity. A lipase from Mucor miehei immobilized on styrene-divinylbenzene copolymer was used to catalyze the direct esterification of butyl alcohol and butyric acid.