2020
DOI: 10.1021/acs.jafc.9b07009
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(Poly)phenols, Carotenoids, and Tocochromanols in Corn (Zea mays L.) Kernels As Affected by Phosphate Fertilization and Sowing Time

Abstract: Corn (Zea mays L.) growth and development is often limited by the availability of phosphate. We thus hypothesized that phosphate fertilization may increase the contents of (poly)­phenols, carotenoids, and tocochromanols (vitamin E) in corn grains. Corn plants cultivated on a soil fertilized with 44 kg phosphorus/ha were compared to plants grown on soil with low plant-available phosphate (1.6 mg CAL-P/100 g of soil), each sown early (April) and late (May) in a randomized field experiment. HPLC-DAD-(HR)-ESI-MS n… Show more

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Cited by 26 publications
(26 citation statements)
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“…The running protocol consisted of two conditions. The first condition was according to the modified method of Lux et al [ 68 ]. Reverse-phase column chromatography was performed using an Ultra Aqueous C18 (250 × 4.6 mm, 5 μm) (RESTEK, Bellefonte, PA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…The running protocol consisted of two conditions. The first condition was according to the modified method of Lux et al [ 68 ]. Reverse-phase column chromatography was performed using an Ultra Aqueous C18 (250 × 4.6 mm, 5 μm) (RESTEK, Bellefonte, PA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Carotenoids were extracted and analyzed in triplicate by HPLC as previously described (Lux et al., 2020). Briefly, 100 mg of flour was weighed into a centrifuge tube.…”
Section: Methodsmentioning
confidence: 99%
“…The extracts (10 mg) were dissolved in ethanol and filtrated through a 0.45 µm syringe filter into a vial bottle. The analysis was performed according to the already reported methods with some modifications [47,75] and analyzed using an Agilent 1260 Infinity II series, coupled with an electrospray ion (ESI) quadrupole mass spectrometry 6130 (Agilent Tech., Santa Clara, CA, USA). Reverse-phase column chromatography was performed using the Restek Ultra C18 column (250 × 4.6 mm, 4.6 mm, 5 µm) (Restek, Bellefonte, PA, USA).…”
Section: Total Polysaccharide Contentmentioning
confidence: 99%