Poly[(R)-3-hydroxybutyrate] production under different salinity conditions by a novel Bacillus megaterium strain

Rodríguez-Contreras, Adrián; Koller, Martin; Braunegg, Gerhart; Marqués-Calvo, María Soledad

doi:10.1016/j.nbt.2015.08.006

Cited by 44 publications

(27 citation statements)

References 22 publications

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“…This strain, originally isolated from the Uyuni salt lake in Bolivia [79], features high capacity of PHB production in typical nutrient media used in industrial biotechnology [80]. The impact of fluctuating salinity on growth and PHB production by this strain was assessed under different NaCl concentrations selected according to the salinities dominant in the strain's natural salt lake environment [81].…”

Section: Discussionmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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The article reviews the current state of knowledge of the production of polyhydroxyalkanoate (PHA) biopolyesters under extreme environmental conditions.Although PHA production by extremophiles is not realized yet at industrial scale, significant PHA accumulation under high salinity or extreme pH-or temperature conditions was reported for diverse representatives of the microbial domains of both Archaea and Bacteria. Several mechanisms were proposed to explain the mechanistic role of PHA and their monomers as microbial cell-and enzyme protective chaperons and the factors boosting PHA biosynthesis under environmental stress conditions. The potential of selected extremophile strains, isolated from extreme environments like glaciers, hot springs, saline brines, or from habitats highly polluted with heavy metals or solvents, for efficient future PHA production on an industrially relevant scale is assessed based on the basic data available in the scientific literature. The article reveals that, beside the needed optimization of other cost-decisive factors like inexpensive raw materials or efficient downstream processing, the application of extremophile production strains can drastically safe energy costs, are easily accessible towards long-term cultivation in chemostat processes, and therefore might pave the way towards cost-efficient PHA production, even combined with safe disposal of industrial waste streams. However, further challenges have still to be overcome in terms of strain improvement and process engineering aspects.

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Section: Discussionmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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“…Synthetic substrates have been used by several authors to determine the PHA production potential of different wastes. The influence of high salinity in industrial wastewater was studied by Rodriguez-Contreras et al (2016) and Palmerio-Sanchez et al (2016). Rodriguez-Contreras et al, (2016) used a glucose solution with different concentrations of NaCl to evaluate the effect of salt concentration in PHA production.…”

Section: Waste Carbon Sourcementioning

confidence: 99%

Challenges of scaling-up PHA production from waste streams. A review

Rodríguez-Pérez

Serrano

Pantión

et al. 2018

Journal of Environmental Management

220

106

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The search for new materials that replace fossil fuel-based plastics has been focused on biopolymers with similar physicochemical properties to fossil fuel-based plastics, such as Polyhydroxyalkanoates (PHA). The present paper reviews the challenges of scaling-up PHA production from waste streams during the period from 2014 to 2016, focusing on the feasibility of the alternatives and the most promising alternatives to its scaling-up. The reviewed research studies mainly focus on reducing costs or obtaining more valuable polymers. In the future, the integration of PHA production into processes such as wastewater treatment plants, hydrogen production or biodiesel factories could enhance its implementation at industrial scale.

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“…Especially exploration of extremophilic microbial strains settling in challenging environments, such as halophile microbes isolated from salt lakes, saltworks, or marine waters, is strongly increasing these days. Application of such production strains enables designing simple, flexible and robust cultivation processes, which can be energy-saving due to low or even without any sterility requirements (32)(33)(34)(35).…”

Section: Polyhydroxyalkanoates (Pha) -A Biological Alternativementioning

confidence: 99%

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

Koller

Braunegg

2018

The EuroBiotech Journal

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Polyhydroxyalkanoates (PHA), the only group of "bioplastics" sensu stricto, are accumulated by various prokaryotes as intracellular "carbonosomes". When exposed to exogenous stress or starvation, presence of these microbial polyoxoesters of hydroxyalkanoates assists microbes to survive."Bioplastics" such as PHA must be competitive with petrochemically manufactured plastics both in terms of material quality and manufacturing economics. Cost-effectiveness calculations clearly show that PHA production costs, in addition to bioreactor equipment and downstream technology, are mainly due to raw material costs. The reason for this is PHA production on an industrial scale currently relying on expensive, nutritionally relevant "1 st -generation feedstocks", such as like glucose, starch or edible oils. As a way out, carbon-rich industrial waste streams ("2 nd -generation feedstocks") can be used that are not in competition with the supply of food; this strategy not only reduces PHA production costs, but can also make a significant contribution to safeguarding food supplies in various disadvantaged parts of the world. This approach increases the economics of PHA production, improves the sustainability of the entire lifecycle of these materials, and makes them unassailable from an ethical perspective.In this context, our EU-funded projects ANIMPOL and WHEYPOL, carried out by collaborative consortia of academic and industrial partners, successfully developed PHA production processes, which resort to waste streams amply available in Europe. As real 2 nd -generation feedstocks", waste lipids and crude glycerol from animal-processing and biodiesel industry, and surplus whey from dairy and cheese making industry were used in these processes. Cost estimations made by our project partners determine PHA production prices below 3 € (WHEYPOL) and even less than 2 € (ANIMPOL), respectively, per kg; these values already reach the benchmark of economic feasibility.The presented studies clearly show that the use of selected high-carbon waste streams of (agro)industrial origin contributes significantly to the cost-effectiveness and sustainability of PHA biopolyester production on an industrial scale.

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Poly[(R)-3-hydroxybutyrate] production under different salinity conditions by a novel Bacillus megaterium strain

Cited by 44 publications

References 22 publications

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Challenges of scaling-up PHA production from waste streams. A review

Advanced approaches to produce polyhydroxyalkanoate (PHA) biopolyesters in a sustainable and economic fashion

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