Poly(<scp>l</scp>-alanine-<i>co</i>-<scp>l</scp>-threonine succinate) as a Biomimetic Cryoprotectant

Park, Jin Kyung; Park, So-Jung; Jeong, Byeongmoon

doi:10.1021/acsami.3c11260

Cited by 5 publications

(2 citation statements)

References 76 publications

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“…Dimethyl sulfoxide (DMSO), glycerol, and ethylene glycol are commonly used permeable CPAs, but they have shortcomings such as toxicity to cells at high concentrations or difficulty of removal from cells, which limit their widespread application in cell cryopreservation (especially for some precious cells, such as oocytes, red blood cells, spermatid cells, etc. ). − Similarly, the rarity and cytotoxicity of AFPs limit their large-scale application . Consequently, there is an urgent need to develop novel freezing methods and nontoxic nanomaterials that can decrease the size and suppress the growth of ice crystals during cell freezing and rewarming processes to improve the freezing efficiency.…”

Section: Introductionmentioning

confidence: 99%

Probing the Size Effect of Graphene Oxide Nanosheets on Ice Crystal Regulation and Laser-Assisted Rapid Rewarming

Li,

Zhang,

et al. 2024

ACS Appl. Mater. Interfaces

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nanomaterials have attracted many researchers to explore the effect of ice control and rapid deicing due to their functional groups, large specific surface area, and excellent photothermal properties. However, the impact of size effects on ice crystal formation, growth, and photothermal performance has been rarely explored. Here, graphene oxide nanosheets (GO NSs) with controllable sizes were used as a representative of 2D nanomaterials to probe the effect of size on ice crystal regulation and rapid rewarming in cell cryopreservation. All sizes of GO NSs exhibited notable inhibitory effects on ice crystal size during the recrystallization process. Significantly, when the size of GO NSs was smaller than a certain size (<150 nm), they showed a more significant ice recrystallization suppression effects, which could reduce the ice crystal size to about 17% of that of pure water. Meanwhile, the photothermal experiments also indicated that smaller-sized GO NSs exhibited better photothermal behavior, with 90 nm GO NSs (GO-90) heating to 70 °C in just 1 min induced by an 808 nm laser (2 W/cm 2 ). Furthermore, applying to cell cryopreservation, cell viability could reach 95.2% and 93% with a low amount of traditional cryoprotectant (2% v/v DMSO) for A549 cells and HeLa cells after recovery, respectively. With the assistance of a 808 nm laser, the rewarming time was also shortened to 20 s, greatly improving the rewarming rate. Our work associated specific sizes of 2D nanomaterials with their ice growth inhibition behaviors during recrystallization and photothermal properties to synergistically improve cell cryopreservation efficiency, providing guidance for effectively designing novel 2D nanomaterials for collaborative control of ice crystals in cell cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Probing the Size Effect of Graphene Oxide Nanosheets on Ice Crystal Regulation and Laser-Assisted Rapid Rewarming

Li,

Zhang,

et al. 2024

ACS Appl. Mater. Interfaces

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“…The scaled-up production of AFPs with a reasonable cost is still difficult. Synthetic polymers have been widely developed as AFP biomimetics, such as poly(vinyl alcohol) (PVA), − poly(ethylene glycol) (PEG), and their block copolymers. , Besides, pronounced IRI activities have also been seen on graphene oxide (GO), nanocelluloses with amphiphilic structures, and facially amphipathic glycopolymers synthesized by ring-opening metathesis polymerization, analogous to natural antifreezing glycoprotein such as poly([( l -Ala) x - co -( l -Thr succinate) y , nanogels, high molecular weight polyproline, oligonucleotides, and surface-functionalized MOF particles . Since synthetic materials are less active than natural AFPs, researchers are turning their focus to the higher-ordered nanostructures .…”

mentioning

confidence: 99%

The Valence-Dependent Activity of Colloidal Molecules as Ice Recrystallization Inhibitors

Tian,

Xu,

Qiu

et al. 2024

ACS Macro Lett.

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Inspired by advances in cryopreservation techniques, which are essential for modern biomedical applications, there is a special interest in the ice recrystallization inhibition (IRI) of the antifreeze protein (AFPs) mimics. There are in-depth studies on synthetic materials mimicking AFPs, from simple molecular structure levels to complex self-assemblies. Herein, we report the valence-dependent IRI activity of colloidal organic molecules (CMs). The CMs were prepared through polymerization-induced particle-assembly (PIPA) of the ABC-type triblock terpolymer of poly(acryloxyethyl trimethylammonium chloride)-b-poly(benzyl acrylate)-b-poly(diacetone acrylamide) (PATAC-b-PBzA-b-PDAAM) at high monomer conversions. Stabilized by the cationic block of PATAC, the strong intermolecular H-bonding and incompatibility of the PDAAM block with PBzA contributed to the in situ formation of Janus particles (AX1) beyond the initial spherical seed particles (AX0), as well as the high valency clusters of linear AX2 and trigonal AX3. Their distribution was controlled mainly by the polymerization degrees (DPs) of PATAC and PDAAM blocks. IRI activity results of the CMs suggest that the higher fraction of AX1 results in the better IRI activity. Increasing the fraction of AX1 from 27% to 65% led to a decrease of the mean grain size from 39.8% to 10.9% and a depressed growth rate of ice crystals by 58%. Moreover, by replacing the PDAAM block with the temperature-responsive one of poly(N-isopropylacrylamide) (PNIPAM), temperature-adjustable IRI activity was observed, which is well related to the reversible transition of AX0 to AX1, providing a new idea for the molecular design of amphiphilic polymer nanoparticle-based IRI activity materials.

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Development of Macromolecular Cryoprotectants for Cryopreservation of Cells

Yuan,

Chen,

Zhu

et al. 2024

Macromol. Rapid Commun.

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Cryopreservation is a common way for long‐term storage of therapeutical proteins, erythrocytes, and mammalian cells. For cryoprotection of these biosamples to keep their structural integrity and biological activities, it is essential to incorporate highly efficient cryoprotectants. Currently, permeable small molecular cryoprotectants such as glycerol and dimethyl sulfoxide dominate in cryostorage applications, but they are harmful to cells and human health. As acting in the extracellular space, membrane‐impermeable macromolecular cryoprotectants, which exert remarkable membrane stabilization against cryo‐injury and are easily removed post‐thaw, are promising candidates with biocompatibility and feasibility. Water‐soluble hydroxyl‐containing polymers such as poly(vinyl alcohol) and polyol‐based polymers are potent ice recrystallization inhibitors, while polyampholytes, polyzwitterions, and bio‐inspired (glyco)polypeptides can significantly increase post‐thaw recovery with reduced membrane damages. In this review, the synthetic macromolecular cryoprotectants are systematically summarized based on their synthesis routes, practical utilities, and cryoprotective mechanisms. It provides a valuable insight in development of highly efficient macromolecular cryoprotectants with valid ice recrystallization inhibition activity for highly efficient and safe cryopreservation of cells.

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Poly(l-alanine-co-l-threonine succinate) as a Biomimetic Cryoprotectant

Cited by 5 publications

References 76 publications

Probing the Size Effect of Graphene Oxide Nanosheets on Ice Crystal Regulation and Laser-Assisted Rapid Rewarming

Probing the Size Effect of Graphene Oxide Nanosheets on Ice Crystal Regulation and Laser-Assisted Rapid Rewarming

The Valence-Dependent Activity of Colloidal Molecules as Ice Recrystallization Inhibitors

Development of Macromolecular Cryoprotectants for Cryopreservation of Cells

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