Poly-<scp>l</scp>-lysine-coated PLGA/poly(amino acid)-modified hydroxyapatite porous scaffolds as efficient tissue engineering scaffolds for cell adhesion, proliferation, and differentiation

Zheng, Shuang; Guan, Yonghong; Yu, Haichi; Huang, Ge; Zheng, Changjun

doi:10.1039/c9nj01675a

Cited by 50 publications

(30 citation statements)

References 27 publications

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“…Therefore, the higher the density of CYS in the polymer matrix, the higher is the viability of the cells [25]. In the literature, several articles can be found about the effect of poly-ʟ-lysine on cell adhesion and proliferation [30,58,59]. It was elucidated that this advantageous feature of poly-ʟ-lysine is concentration-dependent, and the amount of poly-ʟ-lysine should be optimized to reach the highest cell viability [30].…”

Section: Application Of the Pasp-xcys(e)-lys Gels For Cell Cultivationmentioning

confidence: 99%

“…According to the literature, various forms of lysine-containing scaffolds are able to enhance both the adhesion and the proliferation of stem cells. The most commonly used scaffolds are based on poly-ʟ-lysine [30,59,60] and poly-ᴅ-lysine [61]. However, LYS was applied as a cross-linker only in a few cases.…”

Section: Application Of the Pasp-xcys(e)-lys Gels For Cell Cultivationmentioning

confidence: 99%

See 1 more Smart Citation

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Juriga¹,

Sipos²,

Hegedűs³

et al. 2019

Beilstein J. Nanotechnol.

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Polymer hydrogels are ideal scaffolds for both tissue engineering and drug delivery. A great advantage of poly(amino acid)-based hydrogels is their high similarity to natural proteins. However, their expensive and complicated synthesis often limits their application. The use of poly(aspartic acid) (PASP) seems an appropriate solution for this problem due to the relatively cheap and simple synthesis of PASP. Using amino acids not only as building blocks in the polymer backbone but also as cross-linkers can improve the biocompatibility and the biodegradability of the hydrogel. In this paper, PASP cross-linked with cystamine (CYS) and lysine-methylester (LYS) was introduced as fully amino acid-based polymer hydrogel. Gels were synthesized employing six different ratios of CYS and LYS. The pH dependent swelling degree and the concentration of the elastically active chain were determined. After reduction of the disulfide bonds of CYS, the presence of thiol side groups was also detected. To determine the concentration of the reactive cross-linkers in the hydrogels, a new method based on the examination of the swelling behavior was established. Using metoprolol as a model drug, cell proliferation and drug release kinetics were studied at different LYS contents and in the presence of thiol groups. The optimal ratio of cross-linkers for the proliferation of periodontal ligament cells was found to be 60−80% LYS and 20−40% CYS. The reductive conditions resulted in an increased drug release due to the cleavage of disulfide bridges in the hydrogels. Consequently, these hydrogels provide new possibilities in the fields of both tissue engineering and controlled drug delivery.

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Section: Application Of the Pasp-xcys(e)-lys Gels For Cell Cultivationmentioning

confidence: 99%

Section: Application Of the Pasp-xcys(e)-lys Gels For Cell Cultivationmentioning

confidence: 99%

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Juriga¹,

Sipos²,

Hegedűs³

et al. 2019

Beilstein J. Nanotechnol.

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“…Current studies to optimize bone implants mainly focus on surface modification or changing topological features to promote stem cell differentiation and induce bone regeneration [7][8][9][10][11] . However, the antibacterial ability of the implants should also be taken into consideration.…”

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confidence: 99%

Graphdiyne-modified TiO2 nanofibers with osteoinductive and enhanced photocatalytic antibacterial activities to prevent implant infection

et al. 2020

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Titanium implants have been widely used in bone tissue engineering for decades. However, orthopedic implant-associated infections increase the risk of implant failure and even lead to amputation in severe cases. Although TiO2 has photocatalytic activity to produce reactive oxygen species (ROS), the recombination of generated electrons and holes limits its antibacterial ability. Here, we describe a graphdiyne (GDY) composite TiO2 nanofiber that combats implant infections through enhanced photocatalysis and prolonged antibacterial ability. In addition, GDY-modified TiO2 nanofibers exert superior biocompatibility and osteoinductive abilities for cell adhesion and differentiation, thus contributing to the bone tissue regeneration process in drug-resistant bacteria-induced implant infection.

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“…Thus, we will focus on discussing those three examples below. Of course, other biopolymer‐based coatings such as PLL, [ 120 ] pectin, [ 121 ] and fibronectin [ 122 ] are used in tissue engineering approaches as well. Moreover, some biopolymer‐based hybrid coatings, e.g., HA/chitosan, [ 123 ] HA/cationized gelatin, [ 124 ] and alginate/chitosan, [ 125 ] have been explored regarding tissue engineering applications.…”

Section: Coatings For Biomedical Applicationsmentioning

confidence: 99%

Biopolymer‐Based Coatings: Promising Strategies to Improve the Biocompatibility and Functionality of Materials Used in Biomedical Engineering

Song

Winkeljann

Lieleg

2020

Adv Materials Inter

103

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It was a physicist, Wolfgang Pauli, who recognized a century ago that “God made the bulk; the surface was invented by the devil.” And indeed, adjusting the surface properties of materials has kept engineers and chemists busy since—and it still does. In the context of biomedical engineering, the key challenge is ensuring the functionality of an artificial object, which is inserted into the human body—an environment that passively and actively rejects foreign materials. Here, recent advances in this area while focusing on those approaches that employ surface coating strategies with biopolymers are summarized.

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Poly-l-lysine-coated PLGA/poly(amino acid)-modified hydroxyapatite porous scaffolds as efficient tissue engineering scaffolds for cell adhesion, proliferation, and differentiation

Abstract: Ideal bone tissue engineering scaffolds should be biocompatible, biodegradable, and mechanically robust and have the ability to regulate cell function.

Cited by 50 publications

References 27 publications

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Graphdiyne-modified TiO2 nanofibers with osteoinductive and enhanced photocatalytic antibacterial activities to prevent implant infection

Biopolymer‐Based Coatings: Promising Strategies to Improve the Biocompatibility and Functionality of Materials Used in Biomedical Engineering

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