Polyacrylamide‐based monolithic capillary column with coating of cellulose tris(3,5‐dimethylphenyl‐carbamate) for enantiomer separation in capillary electrochromatography

Dong, Xianlin; Wu, Ren’an; Dong, Jing; Wu, Minghuo; Zhu, Yan; Zou, Hanfa

doi:10.1002/elps.200700644

Cited by 41 publications

(43 citation statements)

References 44 publications

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“…The result of baseline separation of benzoin demonstrated that the chiral selector of CDMPC was well coated onto the hybrid monolith matrices. Compared to the reported data on the CDMPC coated onto pure silica monolith [25] or organic monolith [30], the resolution and efficiency on this hybrid monolith was remarkably lower. The same result was also observed for enantioseparation of benzoin with CLC, as shown in Fig.…”

Section: Effects Of Coating Conditions On Enantioseparationmentioning

confidence: 60%

“…The poor efficiency in CEC may be due to the following reasons. In this case, a blank section of capillary with 8.5 cm long was required to fabricate a detection window for on-column CEC analysis and detection, which was different from the on-column detection mode in either pure silica monolith [25] or organic monoliths [30] coated with CDMPC. As a result, the CP-silica monolithic matrices at the interface were not homogeneous, perhaps resulting from the solvent evaporation in the sol-gel process.…”

Section: Effects Of Coating Conditions On Enantioseparationmentioning

confidence: 99%

“…However, the drawbacks of silica-based stationary phases are also well known, including a limited pH range of 2-8 in mobile phases due to unstable nature of Si O Si bond in acidic and basic solutions. Zou et al [30] and Park et al [31,32] coated CDMPC onto the hydrophilic poly(acrylamide-co-N,Nmethylene-bisacrylamide) monolith and porous zirconia monolith with confines of fused-silica capillaries for CEC enantioseparation, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Hybrid monolithic columns coated with cellulose tris(3,5-dimethylphenyl-carbamate) for enantioseparations in capillary electrochromatography and capillary liquid chromatography

Lin

Tang

et al. 2012

Journal of Chromatography A

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Section: Effects Of Coating Conditions On Enantioseparationmentioning

confidence: 60%

Section: Effects Of Coating Conditions On Enantioseparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hybrid monolithic columns coated with cellulose tris(3,5-dimethylphenyl-carbamate) for enantioseparations in capillary electrochromatography and capillary liquid chromatography

Lin

Tang

et al. 2012

Journal of Chromatography A

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“…A number of HPLC separations for a large number of chiral compounds in different classes on polysaccharide-immobilized silica [17][18][19][20] and polymer columns. 21 The polysaccharide-based columns can be used in normal phase, polar organic and reversed-phase mode.…”

Section: Introductionmentioning

confidence: 99%

Chiral Separation on Sulfonated Cellulose Tris(3,5-dimethylphenylcarbamate)-coated Zirconia Monolith by Capillary Electrochromatography

Lee¹,

Jang²,

Park³

2012

Bulletin of the Korean Chemical Society

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Sulfonated cellulose tris(3,5-dimethylphenylcarbamate) (SCDMPC)-coated zirconia monolith (ZM) was used as the chiral stationary phase in capillary electrochromatography for separation of enantiomers of ten chiral compounds in acetonitrile (ACN)-phosphate buffer mixtures as the eluent. Influences of the ACN content, buffer concentration and pH on chiral separation have been investigated. Separation data on SCDMPC-ZM have been compared with those on CDMPC-ZM. Resolution factors were better on SCDMPC-ZM than CDMPC-ZM while retention factors were in general shorter on the former than the latter. Best chiral resolutions on SCDMPC-ZM were obtained with the eluent of 50% ACN containing 50 mM phosphate at pH around 4.Key Words : Chiral separation, Zirconia monolith, Sulfonated cellulose 3,5-dimethylphenylcarbamate, Capillary electrochromatography IntroductionCapillary electrochromatography (CEC) is a hybrid technique of HPLC and CE, and has been increasingly utilized in studies on the development and evaluation of separation methods including chiral separations.1-3 It provides high efficiency because of the flat profile of electroosmotic flow (EOF) to pump the mobile phase and ability to separate charged as well as uncharged compounds through electrophoresis and chromatographic separation.3 Several reviews have been reported on enantioseparations using CEC as a separation technique.1-4 Three types of columns including, open-tubular, particulate-packed and monolithic capillaries are used for CEC.5 Monolithic columns are becoming attractive alternative to particle-packed columns in HPLC and electrochromatography.6-8 Monolithic columns are devoid of problems and difficulties associated with packed capillary columns, including burdensome packing of stationary phase particles in a capillary and frits that cause formation of air bubbles during the analysis which results in reduction of separation efficiency, and tend to break easily.9-12 The monolithic columns allow fast mass transfer at lower pressure drops, enabling much faster separations. The continuous monolithic bed in the capillary column also allows high linear velocities that enable high throughput screening and fast separations of enantiomers. 6-8,13Among various classes of chiral selectors 14,15 polysaccharides have been widely used as the chiral stationary phase (CSP). A great number of polysaccharide derivatives including cellulose, amylose, chitin, chitosan, galactosamine, curdlan, dextran, xylan, and inulin have been in use for chiral separations. 16 The derivatives of cellulose and amylose usually exhibit higher chiral recognition ability than the other type polysaccharides. A number of HPLC separations for a large number of chiral compounds in different classes on polysaccharide-immobilized silica 17-20 and polymer columns.21 The polysaccharide-based columns can be used in normal phase, polar organic and reversed-phase mode. 22While silica-based stationary phases have received wide acceptance due to their well-studied surface chemistry, drawbacks of silica-ba...

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“…HILIC [46] [47] Fused-Silica-Polyacrylamide-based Enantiomer separation CEC [48] Fused-vinylbenzyl trimethylammonium-cyclodextin-based Acidic compound screening CEC [49] β-Cyclodextrin-silica based Enantioseparation LC [50] Zwitterionic Organic-Silica-based Polar compounds HILIC [51] IEC: Ion-Exchange Chromatographic; CEC: Capillary Electrochromatography; AEC: Anion Exchange Chromatography; HPAC: High-Performance Affinity Chromatography; LC: Liquid chromatography; HIC: Hydrophilic interaction chromatography; HILIC: Hydrophilic-Interaction Liquid Chromatography.…”

Section: Introductionmentioning

confidence: 99%

Nano-Doped Monolithic Materials for Molecular Separation

et al. 2017

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Monoliths are continuous adsorbents that can easily be synthesised to possess tuneable meso-/macropores, convective fluid transport, and a plethora of chemistries for ligand immobilisation. They are grouped into three main classes: organic, inorganic, and hybrid, based on their chemical composition. These classes may also be differentiated by their unique morphological and physicochemical properties which are significantly relevant to their specific separation applications. The potential applications of monoliths for molecular separation have created the need to enhance their characteristic properties including mechanical strength, electrical conductivity, and chemical and thermal stability. An effective approach towards monolith enhancement has been the doping and/or hybridization with miniaturized molecular species of desirable functionalities and characteristics. Nanoparticles are usually preferred as dopants due to their high solid phase dispersion features which are associated with improved intermolecular adsorptive interactions. Examples of such nanomaterials include, but are not limited to, carbon-based, silica-based, gold-based, and alumina nanoparticles. The incorporation of these nanoparticles into monoliths via in situ polymerisation and/or post-modification enhances surface adsorption for activation and ligand immobilisation. Herein, insights into the performance enhancement of monoliths as chromatographic supports by nanoparticles doping are presented. In addition, the potential and characteristics of less common nanoparticle materials such as hydroxyapatite, ceria, hafnia, and germania are discussed. The advantages and challenges of nanoparticle doping of monoliths are also discussed.

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Polyacrylamide‐based monolithic capillary column with coating of cellulose tris(3,5‐dimethylphenyl‐carbamate) for enantiomer separation in capillary electrochromatography

Cited by 41 publications

References 44 publications

Hybrid monolithic columns coated with cellulose tris(3,5-dimethylphenyl-carbamate) for enantioseparations in capillary electrochromatography and capillary liquid chromatography

Hybrid monolithic columns coated with cellulose tris(3,5-dimethylphenyl-carbamate) for enantioseparations in capillary electrochromatography and capillary liquid chromatography

Chiral Separation on Sulfonated Cellulose Tris(3,5-dimethylphenylcarbamate)-coated Zirconia Monolith by Capillary Electrochromatography

Nano-Doped Monolithic Materials for Molecular Separation

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