Polyacrylamide–gelatine carrier system used for invertase immobilization

Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: Characterization and application for enzymatic inhibition assays

“…Immobilized enzymes showed a slight optimum pH shift toward alkaline value. A similar shift in pH optimum of lipase immobilized on other solid supports has been reported in the literature [26]. As can be seen in Fig.…”

A simple method for biocatalyst immobilization using PVA‐based hydrogel particles

“…12,16,18,25,26 The roughly 1.8-fold increase in K M following immobilization compares favorably with the K M increases previously reported (1.9 to 3.60) except for the data reported by Bagal and Karve. 25 Curiously, the greatest increase in K M was observed for invertase entrapped in lens-shaped particles, despite their thinness, in the range 200-400 µm, which is suggestive of reduced diffusion limitations.…”

“…14 -16 The invert sugar mixture obtained through enzymatic sucrose hydrolysis has the advantage of being colorless, as opposed to the colored products obtained through acid hydrolysis, mostly due to the presence of hydroxyl methyl furfural. 17,18 The use of an immobilized biocatalyst is favored in this particular application, to prevent product contamination with protein matter, reducing operational costs, increasing the stability of the enzyme, and enabling repeated use or a continuous mode of operation. 17 -19 Although most research work aimed at the improvement of this bioconversion process has focused on the use of immobilized invertases, a different approach, based on the use of inulinase with activity for sucrose hydrolysis, has also been suggested.…”

Optimization of the immobilization process of β-galatosidade by combined entrapment-cross-linking and the kinetics of lactose hydrolysis

“…The actual concentrations of the variables at the maximization point were as follows: X 1 = 6.60% alginate (w/v); X 2 = 4.05% gelatin (w/v) and X 3 = 3.64% glutaraldehyde (v/v). Under these conditions, the immobilization yield, defined as the ratio of the enzyme activity of the immobilized enzyme to the total activity of the soluble enzyme used (Emregul et al, 2006, Nogales et al, 2006, was 30%. A further analysis of the results shown in Figures 1, 2 and 3, as well as in Table 2, revealed that the maximum enzyme activity was obtained in experiment 10, in which the alginate concentration was 6.75% and the glutaraldehyde and gelatin concentrations were 4.03%.…”